生物技术通报 ›› 2025, Vol. 41 ›› Issue (3): 282-293.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0969

• 研究报告 • 上一篇    

基于全基因组SNP位点和性状特征解析我国野生肺形侧耳的遗传多样性

岳园园1,2(), 胡哲源2, 何琪2, 黄晨阳2, 郑素月1(), 赵梦然2()   

  1. 1.河北工程大学园林与生态工程学院,邯郸 056038
    2.中国农业科学院农业资源与农业区划研究所 农业农村部农业微生物资源收集与保藏重点实验室 北方干旱半干旱耕地高效利用全国重点实验室,北京 100081
  • 收稿日期:2024-10-02 出版日期:2025-03-26 发布日期:2025-03-20
  • 通讯作者: 赵梦然,博士,副研究员,研究方向 :食用菌遗传育种与栽培;E-mail: zhaomengran@caas.cn
    郑素月,博士,教授,研究方向 :食药用真菌;E-mail: zhengsuyue@sina.com
  • 作者简介:岳园园,硕士研究生,研究方向 :食用菌种质资源评价与创新利用;E-mail: 461705723@qq.com
  • 基金资助:
    国家重点研发计划(2022YFD1200600);现代农业产业技术体系建设专项(CARS20)

Analysis of Genetic Diversity for Wild Germplasm of Pleurotus pulmonarius in China Based on Whole-genome SNP Loci and Trait Characteristics

YUE Yuan-yuan1,2(), HU Zhe-yuan2, HE Qi2, HUANG Chen-yang2, ZHENG Su-yue1(), ZHAO Meng-ran2()   

  1. 1.College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038
    2.National Key Laboratory for Efficient Utilization of Arid and Semi-arid Cultivated Land in North China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2024-10-02 Published:2025-03-26 Online:2025-03-20

摘要:

目的 探究我国野生肺形侧耳(Pleurotus pulmonarius)的遗传变异水平,以期为新品种选育提供优异亲本材料和数据支撑。 方法 利用全基因组重测序技术获得的SNP标记对37个野生肺形侧耳菌株进行遗传多样性分析,同时观测其菌丝培养特征和结实性等表型性状。 结果 在供试野生菌株中检测到的SNP数量范围在71 521-114 390个之间,菌株间遗传距离的平均值为0.180;聚类分析发现,大部分来自同一采集地点的菌株亲缘关系更为接近,与云南相比,采自陕西、四川、新疆地区的菌株亲缘关系较为接近。野生菌株在菌丝生长适宜温度、菌丝温度敏感性、菌丝恢复生长等培养特征上以及原基形成和分化、子实体成熟、菌盖颜色等结实特性方面呈现出丰富的表型多样性,基于表型特征的聚类分析结果表明,所有菌株在SM相似系数为0.52水平上能够按照原基能否正常分化分成两大组,这与主成分分析的类群划分结果高度一致。 结论 我国肺形侧耳野生种质资源遗传多样性丰富,具有良好的驯化利用潜力,野生菌株基因组水平上的遗传分化程度可能与其地理分布有关,与表型特征无明显相关性。

关键词: 秀珍菇, 遗传变异, 单核苷酸多态性, 表型特征

Abstract:

Objective The genetic variation of the wild resources in Pleurotus pulmonarius was analyzed, aiming to provide the excellent parent strains and data support for the breeding of new varieties. Method A total of 37 wild strains were used to analyze the genetic diversity based on SNP loci obtained by whole genome resequencing, and to observe the phenotypic traits such as cultural characteristics and fruiting characteristics. Result The number of SNPs detected in the wild strains ranged from 71 521 to 114 390, and the average genetic distance between strains was 0.180. Cluster analysis showed that the closer genetic relationship among most strains from the same location, and those was closer among the strains from Shaanxi, Sichuan and Xinjiang regions in comparison with the strains from Yunnan. Abundant phenotypic diversity in culture characteristics such as favorable temperature, temperature sensitivity, colony recovery, and fruiting characteristics such as primordium formation and differentiation, fruiting body maturation, and cap color were observed in wild resources of P. pulmonarius. The cluster analysis based on phenotypic characteristics showed that all strains were divided into two groups according to primordium differentiation at the similarity coefficient of 0.52, which was highly consistent with the classification results of the principal component analysis. Conclusion The genetic variation in the wild germplasm of P. pulmonarius is abundant,which have fine potential for domestication and utilization. The degree of genetic differentiation at the genome level among wild strains might be related to their geographical distributions rather than their phenotypic characteristics.

Key words: Xiuzhengu, genetic variation, single nucleotide polymorphism, phenotypic characteristics