樟树MYC转录因子的全基因组鉴定与表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2025-0889

摘要/Abstract

摘要：

目的 MYC转录因子是茉莉酸（JA）信号通路的核心调节因子，在调控植物生长发育和次生代谢过程中发挥重要作用。鉴定和分析樟树中的MYC转录因子，为研究萜类等次生代谢产物合成以及生长发育提供依据。方法采用生物信息学方法，对CcMYC家族进行全基因组鉴定，分析其染色体定位、理化性质、亚细胞定位、家族进化关系、基因结构、启动子和PPI网络，并结合实时荧光定量PCR分析CcMYC基因家族在樟树6种化学型中的表达模式。结果 CcMYC1-CcMYC12不均匀地分布在10条染色体上。CcMYC家族编码蛋白含629个氨基酸，平均分子质量69.64 kD，理论等电点5.15-6.69，二级结构以α-螺旋和无规则卷曲为主。蛋白的亚细胞定位结果显示，CcMYC12主要定位于细胞核。系统进化分析结果表明，CcMYC基因分属于不同的3个组；种间共线性分析表明，CcMYC基因存在片段重复事件，CcMYC1、CcMYC4和CcMYC5在拟南芥、水稻和毛果杨中均存在共线基因；启动子顺式作用元件分析表明，CcMYC基因可能受到光照、多种植物激素以及防御与胁迫的诱导。蛋白互作分析揭示，CcMYC3、CcMYC7、CcMYC8、CcMYC11可能在调控樟树类黄酮、萜类等次生代谢物质合成积累中发挥重要作用。结论从樟树基因组中共鉴定出12个MYC家族成员，CcMYC1、CcMYC8在右旋龙脑型和柠檬醛型中高表达，CcMYC11、CcMYC12在芳樟醇型中高表达，它们可能在不同化学型樟树精油主要成分合成与萜类等次生代谢积累中发挥重要作用。

关键词: 樟树, MYC转录因子, 次生代谢, 生物信息学分析, 全基因组鉴定

Abstract:

Objective MYC transcription factors (TFs) are core regulators of the jasmonic acid (JA) signalling pathway and play crucial roles in modulating plant growth, development, and secondary metabolism. Identification and analysis of MYC TFs in Cinnamomum camphora will provide a theoretical basis for investigating the biosynthesis of terpenoids and other secondary metabolites, as well as plant growth and development. Method Bioinformatics approaches were employed to perform genome-wide identification of the CcMYC family, including chromosomal localization, physicochemical properties, subcellular localization, phylogenetic relationships, gene structure, promoter regions, and protein-protein interaction (PPI) networks. Combined with quantitative real-time PCR (RT-qPCR), the expression patterns of the CcMYC gene family in six chemotypes of C. camphora was analyzed. Result CcMYC1-CcMYC12 were unevenly distributed across the 10 chromosomes. Proteins encoded by the CcMYC family contained 629 amino acids, an average molecular mass of 69.64 kD, and theoretical isoelectric points ranging from 5.15 to 6.69; their secondary structures were mainly composed of α-helices and random coils. Subcellular localization prediction showed that CcMYC12 was predominantly localized in the nucleus. Phylogenetic analysis revealed that the CcMYC genes were clustered into three distinct groups. Interspecific collinearity analysis indicated that segmental duplication events occurred in the CcMYC gene family, and collinear orthologs of CcMYC1, CcMYC4 and CcMYC5 were identified in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa, respectively. Analysis of cis-acting elements in the promoter regions suggested that the expressions of CcMYC genes may be induced by light, diverse plant hormones, as well as defense and stress stimuli. Protein-protein interaction (PPI) analysis further revealed that CcMYC3, CcMYC7, CcMYC8 and CcMYC11 may play vital roles in regulating the biosynthesis and accumulation of secondary metabolites including flavonoids and terpenoids in C. camphora. Conclusion A total of 12 MYC family members have been identified from the genome of C. camphora. Among them, CcMYC1 and CcMYC8 are highly expressed in the D-borneol chemotype and citral chemotype, while CcMYC11 and CcMYC12 exhibit high expressions in the linalool chemotype. These genes may contribute significantly to the biosynthesis and accumulation of essential oil main components, terpenoids and other secondary metabolites in different chemotypes of C. camphora.

Key words: Cinnamomum camphora, MYC transcription factor, secondary metabolism, bioinformatics analysis, genome-wide identification

辛晴, 郑永杰, 李雨华, 涂白连, 刘新亮, 张月婷, 伍艳芳. 樟树MYC转录因子的全基因组鉴定与表达分析[J]. 生物技术通报, 2026, 42(7): 214-225.

XIN Qing, ZHENG Yong-jie, LI Yu-hua, TU Bai-lian, LIU Xin-liang, ZHANG Yue-ting, WU Yan-fang. Genome-wide Identification and Expression Analysis of MYC Transcription Factors in Cinnamomum camphora[J]. Biotechnology Bulletin, 2026, 42(7): 214-225.

图/表 11

表1 樟树MYC基因家族二级蛋白结构

Table 1 Secondary structure of MYC gene family proteins in licorice

基因名称 Gene name	α-螺旋 Alpha helix （%）	β-转角 Beta turn （%）	扩展链 Extended strand （%）	无规则卷曲 Random coil （%）
CcMYC1	197（20.10）	2（0.30）	41（6.06）	437（64.55）
CcMYC2	186（26.42）	9（1.28）	42（5.97）	467（66.34）
CcMYC3	169（32.63）	1（1.93）	52（10.04）	287（55.41）
CcMYC4	191（28.81）	1（1.96）	43（6.49）	416（62.75）
CcMYC5	182（28.53）	6（0.94）	44（6.90）	406（63.64）
CcMYC6	192（30.48）	2（0.32）	46（7.30）	390（61.90）
CcMYC7	151（26.73）	2（0.35）	37（6.55）	375（66.37）
CcMYC8	146（30.61）	8（1.68）	46（9.64）	277（58.07）
CcMYC9	180（29.70）	4（0.66）	49（8.09）	373（61.55）
CcMYC10	206（30.61）	7（1.04）	57（8.47）	403（59.88）
CcMYC11	233（32.41）	9（1.25）	51（7.09）	426（59.25）
CcMYC12	217（33.28）	7（1.07）	41（6.29）	387（59.36）

图1 樟树与拟南芥、毛果杨MYC转录因子进化分析

Fig. 1 Phylogenetic analysis of the MYCtranscription factor in C. camphora, A. thaliana, and P. trichocarpa

图2 樟树MYC蛋白三级结构预测

Fig. 2 Tertiary structure prediction of licorice MYC protein

图3 樟树MYC成员染色体定位分析

Fig. 3 Chromosomal localization analysis of MYC family members in C. camphora

图4 樟树MYC基因家族共线性分析

Fig. 4 Collinearity analysis of MYC gene family in C. camphora

图5 樟树与拟南芥、水稻MYC基因家族的共线性分析

Fig. 5 Collinearity analysis of MYC gene family among C. camphora, A. thaliana and O. sativa

图6 樟树MYC基因家族的保守基序、保守结构域与基因结构分析

Fig. 6 Conserved motifs, conserved domains, and gene structure analysis of MYC genes family in C. camphora

图7 樟树MYC基因家族启动子顺式原件预测

Fig.7 Prediction of cis-elements of the MYC gene family promoter in C. camphora

图8 拟南芥与樟树MYC蛋白相互作用网络分析

Fig. 8 Protein interaction network of orthologous MYC proteins in A. thaliana and C. camphora

图9 樟树MYC基因在6种化学型中的表达量不同小写字母代表差异显著（P<0.05）

Fig. 9 Expressions of the MYC gene in six chemotypes of C. camphoraDifferent lowercase letters denote statistically significant differences (P<0.05)

图10 CcMYC12亚细胞定位

Fig. 10 Subcellular localization of CcMYC12

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