逆境对红酵母发酵生产类胡萝卜素的促进作用

生物技术通报 ›› 2013, Vol. 0 ›› Issue (1): 191-197.

逆境对红酵母发酵生产类胡萝卜素的促进作用

史馨怡 ,刘丹 ,陈黎黎 ,陈欣

江苏科技大学生物与化学工程学院，镇江 212018

收稿日期:2012-07-02 修回日期:2013-01-31 出版日期:2013-01-30 发布日期:2013-01-30
作者简介:史馨怡，女，研究方向：发酵工程；E-mail: shixinyi9820@163.com

Enhanced Carotenoids Production by a Rhodotorula sp. Under Stress Conditions

Shi Xinyi, Liu Dan, Chen Lili, Chen Xin

School of Biological and Chemical Engineering，Jiangsu University of Science and Technology，Zhenjiang 212018

Received:2012-07-02 Revised:2013-01-31 Published:2013-01-30 Online:2013-01-30

摘要/Abstract

摘要： 类胡萝卜素广泛存在于细菌、藻类、真菌和植物中，是一类具有重要的生理保健功能的呈黄色、橙红色或红色的多烯类化合物。考察了不同逆境条件，包括低温、低温处理时间、酸处理及高浓度盐处理对促进一株红酵母（Rhodotorula sp.）发酵生产类胡萝卜素的影响，并利用响应面分析方法研究了其交互作用。结果表明，低温，酸处理、高浓度盐的影响以及低温和酸处理、低温和高浓度盐以及低温处理时间和酸处理的交互作用对类胡萝卜素产量的影响均显著，在温度为15℃处理48 h，pH3.5，NaCl 浓度为2 mol/L 的条件下，类胡萝卜素最高产量达到31.04 mg/L，说明逆境对红酵母发酵生产类胡萝卜素具有促进作用。

关键词: 红酵母 , 类胡萝卜素 , 逆境 , 响应面分析

Abstract: Carotenoids, displaying yellow, orange, and red color, are a series of polyene compounds with important physiologicalhealthy function. They are found widely in microorganisms, including algae and plants. The effects of four factors, including low temperature,treatment time at low temperature, acid treatment and high-concentration salt treatment, on the carotenoids production by a Rhodotorula sp. wereinvestigated. Based on this, response surface analysis method was used to elucidate the interaction of the four factors. The results showed thatthe effects of low temperature, acid, high-concentration salt treatment and the interactive effects of low temperature-acid, low temperature- highconcentrationsalt, treatment time at low temperature-acid on carotenoids production are significant. The maximum carotenoids yield of 31.04 mg/Lwas obtained at the low temperature of 15 ℃ for 48 h, with pH 3.5 and 2 mol/L NaCl, which indicates that the carotenoids production can beenhanced under stress conditions.

Key words: Rhodotorula sp. , Carotenoids , Stress conditions , Response surface analysis

史馨怡, 刘丹, 陈黎黎, 陈欣. 逆境对红酵母发酵生产类胡萝卜素的促进作用[J]. 生物技术通报, 2013, 0(1): 191-197.

Shi Xinyi, Liu Dan, Chen Lili, Chen Xin. Enhanced Carotenoids Production by a Rhodotorula sp. Under Stress Conditions[J]. Biotechnology Bulletin, 2013, 0(1): 191-197.

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