腺苷形成酶家族研究进展

摘要/Abstract

摘要： ANL 腺苷形成酶家族（ANL Adenylating Enzymes）包含有酰基- 和芳香基-CoA 合成酶，荧光素酶和非核糖体肽合成酶中的腺苷功能域。这个家族酶催化两步反应：首先是羧酸底物腺苷化，形成酰基-AMP 中间产物；第二步为硫酯键的形成。随着大量的ANL 家族成员不断被鉴别，科学家们提出10 个保守的序列基序，它们被认为与酶催化活性相关。大量的晶体结构分析和动力学试验证明，在酶催化过程中，C 末端亚基的旋转发挥了重要的作用。这种旋转是ANL 家族酶重要的催化特点，其独特之处在于允许酶利用同样的活性位点表现出不同的构象并催化完全不同的两步反应。综述腺苷形成酶超家族的3 种酶系，并分别从研究历程、保守区域及反应过程中的酶构象动力学进行介绍。

关键词: 腺苷形成酶家族, 酰基-CoA, 合成酶, 荧光素酶, 腺苷功能域, 构象变化

Abstract: The ANL adenylating enzymes contains acyl- and aryl-CoA synthetases, firefly luciferase, and the adenylation domains of the modular non-ribosomal peptide synthetases（NRPSs）. Members of this family catalyze a two-step reaction ：the initial adenylation of a carboxylate to form an acyl-AMP intermediate, the second step commonly for formation of a thioester. With more members of the adenylateforming family of enzymes being identified, 10 conserved and important sequence motifs for catalytic activity have been revealed. The comprehensive studies of the structures and kinetics provide insight into the role of the rotation of the C-terminal sub-domain in the catalytic cycle. Such a rotation, a common strategy used by ANL adenylating enzymes, can present different conformation of the active sites to catalyze different partial reactions. In this paper，we describe the three types of ANL adenylating enzymes and summarize the research course of the ANL superfamily enzymes，conserved regions and conformational dynamics of enzymes in the process of the enzymatic reactions.

Key words: Adenylating enyzmes, Acyl-CoA synthetases, Firefly luciferase, Adenylation domain, Conformational dynamics

李丽丽林双君. 腺苷形成酶家族研究进展[J]. 生物技术通报, 2013, 0(4): 14-20.

Li Lili, Lin Shuangjun. Recent Advances in ANL Andenylating Enzymes[J]. Biotechnology Bulletin, 2013, 0(4): 14-20.

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