棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 94-98.

棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响

欧秀玲, 耿雅文, 李凤, 李曹龙, 祝建波, 王爱英

(石河子大学生命科学学院农业生物技术重点试验室, 石河子 832003)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:欧秀玲, 女, 硕士研究生, 研究方向: 植物基因工程;E-mail: 1498739259@qq.com
基金资助:
转基因专项(2011ZX08011-002)

The Effect of Physiological Traits of Transgenic Cotton Induced by Verticillium wilt Pathogen

Ou Xiuling, Geng Yawen, Li Feng, Li Caolong, Zhu Jianbo, Wang Aiying

(Key Laboratory of Agriculture Biotechnology, College of Life Science Shihezi University, Shihezi 832003)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： 通过研究棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响, 旨在为提高转基因抗病棉花抗病性提供理论依据。Byd、28p两品种棉花为供试材料, 应用花粉管通道法将几丁质酶与β-1, 3-葡聚糖双价抗病基因导入棉花株系中, 多年筛选获得不同抗性水平的棉花株系;棉种经棉花黄萎病病菌侵染后, 苗期测定棉株内的生理生化变化。结果显示, 转基因抗病棉株体内的酶活性已达对照水平, 而感病及耐病棉株内的酶活性均有不同程度的变化。所测定的棉株内的酶活性及脯氨酸含量的变化与棉株的抗病性相关。

关键词: 棉花黄萎病, 抗病, 酶活性, 生理指标

Abstract: To provide theoretical basis for improving resistant in transgenic cotton by researching the effect of physiological traits of transgenic cotton induced by Verticillium wilt pathogen. Chitinase and β-1, 3-glucan of bivalent resistance genes were delivered to two cotton lines of Byd, 28p by pollen-tube pathway method. The transgenic cotton lines with different resistant levels were obtained by many years screening. After the Verticillium wilt pathogen infestation to cotton seeds, the physiological and biochemical changes in transgenic cotton were determined at seedling stage. The results showed that the enzyme activity in transgenic disease-resistant cotton plants had reached the control's level, however, the level of enzyme activity in susceptible and disease-resistan cotton plants were different. The enzyme activity and changes of proline content were related to disease resistance of transgenic cotton plants.

Key words: Verticillium wilt, Disease resistance, Enzyme activity, Physiological index

欧秀玲, 耿雅文, 李凤, 李曹龙, 祝建波, 王爱英. 棉花黄萎病病原菌诱导侵染对转基因抗病棉花生理性状的影响[J]. 生物技术通报, 2013, 0(6): 94-98.

Ou Xiuling, Geng Yawen, Li Feng, Li Caolong, Zhu Jianbo, Wang Aiying. The Effect of Physiological Traits of Transgenic Cotton Induced by Verticillium wilt Pathogen[J]. Biotechnology Bulletin, 2013, 0(6): 94-98.

参考文献

[1] 徐显.棉花抗黄萎病育种研究现状及对策[J].河北农业科学, 2006, 4(2):95-97.
[2] 石磊岩.我国棉花黄萎病研究进展[J].棉花学报, 1995, 7(4):243-245.
[3] 陈其, 李典谟, 曹赤阳.棉花病虫害综合防治及其研究进展[M].北京:中国农业科技出版社, 1990:354-364.
[4] 陈旭升, 陈永萱, 黄骏麒.棉花黄萎病菌株VD8外泌毒蛋白的生化特性[J].江苏农业科学, 1998, 14(2):126-128.
[5] 李成伟, 丁锦平, 刘冬梅, 等.棉花黄萎病及抗病育种研究进展[J].棉花学报, 2008, 20(5):385-390.
[6] 赵凤轩, 戴小枫.棉花黄萎病菌的侵染过程[J].基因组学与应用生物学, 2009, 28(4):786-792.
[7] Devay JE, Weir BL, Wakeman RJ, et al. Effects of Verticillium dahliae infection of cotton plant(Gossy pium L.)on potassium levels in leaf petioles[J]. Plant Disease, 1997, 81(9):1089-1092.
[8] Paplomatas EJ, Bassett DM, Broome JC, et al. Incidence of Verticil-lium wilt and yield losses of cotton cultivars based on soil inoculum density of Physiopathology[J]. Ecology and Epidemiology, 2007, 82(12):1417-1420.
[9] Natasha Robinson, Platt HW, Hale LR. Interactions of various Vert-icillium species in combination with V. albo-atrum on Verticillium wilt disease development in potato[J]. American Journery of Po-tato Resreach, 2007, 84(2):133-141.
[10] 李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社, 2000.
[11] 杨兰芳, 庞静, 彭小兰, 闫静静.紫外分光光度法测定植物过氧化氢酶活性[J].现代农业科学, 2009, 20(3):364-366.
[12] 马旭俊, 朱大海.植物超氧化物歧化酶(SOD)的研究进展[J].遗传, 2003, 25(2):225-231.
[13] 窦俊辉, 喻树迅, 范术丽, 等. SOD与植物胁迫抗性[J].分子植物育种, 2010, 8(2):359-364.
[14] 欧阳光察, 薛应龙.植物苯丙烷类代谢的生理意义及其调控[J].植物生理学通讯, 1988, 24(3):9-16.
[15] 张宪政, 陈凤玉, 王荣富.植物生理学试验技术[M].沈阳:辽宁科技出版社, 1994.
[16] 李盾, 王振中, 林孔勋.花生体内几种酶的活性与抗锈病性的关系[J].南京农业大学报, 1991, 12(3):1-6.
[17] 陈璋.水稻抗稻瘟病与苯丙氨酸解氨酶及过氧化物酶活性的相关性[J].植物生理学通讯, 1993, 29(4):275.
[18] 冯洁, 陈其焕.棉株体内几种生化物质与抗枯萎病之间关系的初步研究[J].植物病理学报, 1991, 21(4):291-297.
[19] 杨家书, 吴畏, 吴友三, 等.植物苯丙氨酸类代谢与小麦白粉病抗性的关系[J].植物病理学报, 1986(3):1-6.
[20] 曾永三, 王振中.苯丙氨酸解氨酶及过氧化物酶活性的相关性与豇豆抗锈病性的关系[J].仲恺农业技术学院学报, 2003, 16(1):1-5.
[21] 马存, 简桂良.我国棉花抗黄萎病育种现状、问题及对策[J].中国农业科学, 1997, 30:58-64.
[22] 王雅平, 刘伊强, 施磊, 等. 小麦对赤霉病抗性不同品种的SOD活性[J]. 植物生理学报, 1993, 19(4):353-358.