五条蚋（Simulium quinquestriatum）不同地理种群遗传多样性的ISSR标记研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (7): 107-113.

五条蚋（Simulium quinquestriatum）不同地理种群遗传多样性的ISSR标记研究

修江帆,张春林,陈汉彬

（贵阳医学院生物学教研室，贵阳 550004）

收稿日期:2013-01-15 修回日期:2013-07-19 出版日期:2013-07-19 发布日期:2013-09-02
作者简介:修江帆, 男, 博士研究生, 讲师, 研究方向: 医学昆虫分子生物学；E-mail：xiujiangfan@163.com
基金资助:
国家自然科学基金资助项目（39460073），贵阳医学院青年教师科研基金项目（K2011-39）

Genetic Diversity of Different Geographical Populations of Simulium quinquestriatum Based on ISSR Analysis

Xiu Jiangfan, Zhang Chunlin, Chen Hanbin

（Department of Biology，Guiyang Medical College，Guiyang 550004）

Received:2013-01-15 Revised:2013-07-19 Published:2013-07-19 Online:2013-09-02

摘要/Abstract

摘要： 为阐明五条蚋（S. quinquestriatum）不同地理种群的遗传多样性，应用ISSR技术对我国五条蚋8个地理种群的遗传多样性及其分化进行了研究分析。构建了中国五条蚋8个种群160个体的ISSR指纹图谱。8条引物扩增出111条ISSR条带，其中96条为多态性条带，占总扩增条带数的86.47%，每个种群显示了各自独特的ISSR图谱。ISSR标记的遗传多样性分析结果表明：五条蚋在物种水平表现出较高的遗传多样性（P=86.4%，A=1.991 0±0.094 9，A_E=1.553 2±0.334 5，H=0.323 1±0.157 9，I=0.484 7±0.203 7）；在种群水平表现出较低的遗传多样性（P=78.37%，A=1.492 1±0.499 7，A_E=1.316 6±0.370 0，H=0.180 1±0.206 6，I=0.265 5±0.295 0）；分子方差（AMOVA）分析结果表明五条蚋种群间的遗传分化为59%，种群内部的遗传分化程度为41%；种群间的遗传分化系数（G_st）为0.59，种群间基因流（N_m）为0.347 5。说明五条蚋不同地理种群间遗传分化程度高，地理屏障（高山和平原等）以及栖息地片段化是导致其遗传分化形成的主要因素。

关键词: 五条蚋, 种群, ISSR, 遗传多样性, 遗传分化

Abstract: In this paper, the genetic diversity of geographical populations of S. quinquestriatum from China was investigated with ISSR method using eight selective primers in order to clarify thegenetic diversity of eight different geographic populations of S. quinquestriatum. Establishment of ISSR fingerprint of S. quinquestriatum from eight populations of 160 individuals in this study. The results showed that total 111 bands were generated, of which 96 bands were polymorphic bands（the percentage of polymorphic band, P= 86.47％）. The characteristic ISSR pattern for each individual was detected. S. quinquestriatum displayed the higher degree of genetic diversity at the species level（P=86.4%, A=1.991 0±0.094 9, A _E=1.553 2±0.334 5, H=0.323 1±0.157 9, I=0.484 7±0.203 7）than the population level（P=78.37%, A=1.492 1±0.499 7, A _E=1.316 6±0.370 0, H=0.180 1±0.206 6, I=0.265 5±0.295 0）. Results of AMOVA analysis demonstrated that the among-population component had a high degree which accounted for 59% of the total genetic differentiation；while the within-population component accounted for 41%. The gene flow that among-population is 0.347 5. It is indicated that the main factors for the high degree of genetic differentiation among different geographic populations of S. quinquestriatum are geographic barrier（mountains and plains）and habitat fragmentation.

Key words: Simulium quinquestriatum, Populations ISSR, Genetic diversity, Genetic differentiation

修江帆,张春林,陈汉彬. 五条蚋（Simulium quinquestriatum）不同地理种群遗传多样性的ISSR标记研究[J]. 生物技术通报, 2013, 0(7): 107-113.

Xiu Jiangfan, Zhang Chunlin, Chen Hanbin. Genetic Diversity of Different Geographical Populations of Simulium quinquestriatum Based on ISSR Analysis[J]. Biotechnology Bulletin, 2013, 0(7): 107-113.

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