生物技术通报 ›› 2013, Vol. 0 ›› Issue (8): 43-50.

• 研究报告 • 上一篇    下一篇

新疆短命植物小拟南芥与模式植物拟南芥的抗逆表型比较

张婷婷, 周亚平, 曾幼玲   

  1. (新疆大学生命科学与技术学院 新疆生物资源基因工程重点实验室,乌鲁木齐 830046)
  • 收稿日期:2013-03-13 修回日期:2013-08-11 出版日期:2013-08-11 发布日期:2013-09-02
  • 作者简介:张婷婷,女,研究方向:植物抗逆生理和分子机制;E-mail:ztt12721960@126.com
  • 基金资助:
    国家大学生创新性实验计划项目(101075513),新疆维吾尔自治区高校科研计划项目(XJEDU2011102)

Comparison on Stress-resistent Phenotypes Between Arabidopsis pumila and Arabidopsis thaliana and Expression Analysis

Zhang Tingting, Zhou Yaping, Zeng Youling   

  1. (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046)
  • Received:2013-03-13 Revised:2013-08-11 Published:2013-08-11 Online:2013-09-02

摘要: 比较小拟南芥和拟南芥的抗逆表型试验数据和通过实时荧光定量PCR技术对小拟南芥抗逆基因的表达检测分析,获得小拟南芥优于拟南芥更强的抗逆表型试验数据,以及小拟南芥在胁迫条件下逆境相关基因的表达模式。结果表明(1)在相对含水量相同的情况下,小拟南芥比拟南芥具有更强的保水性。(2)通过幼苗生长的表型观察和相对根长的统计学分析,小拟南芥在盐、模拟干旱、热和ABA激素等胁迫处理下表现出比拟南芥更强的抗逆性;小拟南芥在冷胁迫处理后,恢复正常生长的条件没有表现出较好的耐冷特性。(3)在盐、模拟干旱、冷和ABA多种胁迫处理条件下,一些逆境相关基因RD22RD29AADH在小拟南芥幼苗中的表达绝大多数呈现显著上调趋势。

关键词: 小拟南芥, 拟南芥, 抗逆表型比较, 胁迫相关基因, 表达分析

Abstract: In this study, the data of stress-resistent phenotypes were compared between Arabidopsis pumila and Arabidopsis thaliana and the expression of some stress-responsed genes was examined by Real-time PCR in Arabidopsis pumila subjected to the salt, mimic drought, ABA and cold stress. Some results were as followed:(1)The water-retention capacity of Arabidopsis pumila was stronger than Arabidopsis thaliana under the same condition with the determination of relative water content between two species.(2)Arabidopsis pumila showed more stressed resistence than Arabidopsis thaliana as model plant under salt, mimic drought, heat and ABA stress by the observation on the growth phenotypes and the statistical analysis of relative root length between two species. Arabidopsis pumila didn't present a better growth phenotype than Arabidopsis thaliana after a cold stress treatment, then a normal growth condition.(3)The expressions of some stress-responsed genes in Arabidopsis pumila, such as RD22, RD29A and ADH, had significant increases in most case under the treatments of salt, mimic drought, cold and ABA.

Key words: Arabidopsis pumila, Arabidopsis thaliana, Comparison on stress-resistent phenotypes, Stress-responsed genes, Expres-sion analysis