香山黄栌叶片内生细菌的分离与鉴定

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 151-155.

香山黄栌叶片内生细菌的分离与鉴定

左山¹,刘洋²,周肖红³,沈悦¹,李哲¹,彭程¹,陈亮³,程池²

1. 北京市广渠门中学，北京 100062
2. 中国食品发酵工业研究院中国工业微生物菌种保藏管理中心，北京 100027
3. 北京市香山公园管理处，北京 100093

收稿日期:2013-09-04 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:左山，女，硕士，研究方向: 植物微生物生态学，E-mail: zuoshanwork@163.com ；刘洋同为本文第一作者
基金资助:
北京市青少年科学探索专项资金项目，中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

Isolation and Identification of Endophytic Bacteria in Leaf from Xiangshan Smoke Tree(Cotinus coggygria var. cinerea#br#)

Zuo Shan¹, Liu Yang², Zhou Xiaohong3 Shen Yue¹, Li Zhe¹, Peng Cheng¹, Chen Liang³, Cheng Chi²

1. Beijing Guang Qumen Middle School，Beijing 100062;
2. China Center of Industrial Culture Collection，China National Research Institute of Food and Fermentation Industries，Beijing 100027;
3. Beijing Xiangshan Park Management Office，Beijing 100093

Received:2013-09-04 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 通过微生物培养方法对北京香山黄栌叶片内生细菌进行分离及筛选，共得到81 株内生细菌，经形态学观察、16S#br#rDNA 扩增及系统发育分析，将其归为变形菌门(Proteobacteria)中的欧文氏菌属(Erwinia)和志贺氏菌属(Shigella)；厚壁菌门#br#(Firmicutes)中的芽孢杆菌属(Bacillus)、柯恩氏菌属(Cohnella)、类芽孢杆菌属(Paenibacillus)和葡萄球菌属#br#(Staphylococcus)；#br#放线菌门(Actinobacteria)中的短小杆菌属(Curtobacterium)。这是国内外首次利用培养方法针对黄栌叶片内生细菌进行研究的报#br#道。

关键词: 黄栌, 叶, 内生细菌, 培养方法

Abstract: The endophytic bacteria in leaf of Xiangshan smoke tree were isolated, screened and identified by the microbial culturedependent method combing with morphological observation, 16S rDNA amplification and phylogenetic analysis, indicated that the total 81 strains from the leaves were clustered into 7 genus: Erwinia and Shigella, Bacillus, Cohnella, Paenibacillus and Staphylococcus, and Curtobacterium belonging to Proteobacteria, Firmicutes and Actinobacteria, respectively. This is the first report about investigation on the endophytic bacteria in leaf of Xiangshan smoke tree using culture-dependent method.

Key words: Smoke tree, Leaf, Endophytic bacteria, Culture-dependent method

左山,刘洋,周肖红,沈悦,李哲,彭程,陈亮,程池. 香山黄栌叶片内生细菌的分离与鉴定[J]. 生物技术通报, 2014, 0(1): 151-155.

Zuo Shan, Liu Yang, Zhou Xiaohong3 Shen Yue, Li Zhe, Peng Cheng, Chen Liang, Cheng Chi. Isolation and Identification of Endophytic Bacteria in Leaf from Xiangshan Smoke Tree(Cotinus coggygria var. cinerea#br#)[J]. Biotechnology Bulletin, 2014, 0(1): 151-155.

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