转Bt cry1Ah基因抗虫玉米的分子检测及农艺性状分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 62-68.

转Bt cry1Ah基因抗虫玉米的分子检测及农艺性状分析

戴军^1，2 李秀影² 朱莉² 汪海² 张杰³ 何康来³ 郎志宏² 黄大昉²

(1.西南科技大学生命科学与工程学院，绵阳 621010；2.中国农业科学院生物技术研究所，北京 100081；3. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193)

收稿日期:2014-03-29 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:戴军，男，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：augustdj@126.com
基金资助:
国家自然科学基金项目(30970231)，转基因生物新品种培育重大专项(2013ZX08003-001)

Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene

Dai Jun^1，2 Li Xiuying² Zhu Li² Wang Hai² Zhang Jie³ He Kanglai³ Lang Zhihong² Huang Dafang²

(1. School of Life Science and Engineering，Southwest Science and Technology University，Mianyang 621010；2. Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081；3. State Key Laboratory of Plant Insect and Disease Biology，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193)

Received:2014-03-29 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 利用农杆菌介导法将具有自主知识产权的Bt cry1Ah基因转入玉米自交系综31中，在获得的1 764株转基因植株中筛选获得1株对玉米螟有显著抗性的转基因植株。对该抗性植株进行了室内和田间抗虫性分析及外源基因表达分析，并研究了其杂交后代的农艺性状。结果表明，该转基因植株对亚洲玉米螟田间抗虫性达到高抗水平；外源基因能够正常高效表达，在玉米抽穗期的叶片中Cry1Ah蛋白表达量最高，达到1 μg/g鲜重；株高、穗位高、穗长、穗粗等与非转基因对照植株相比差异不大，秃尖长明显短于对照，千粒重及单株产量明显高于对照，这种差异在人工模拟虫害爆发情况下达到极显著水平。

关键词: 转基因玉米, 种群筛选, 分子检测, 抗虫, 农艺性状

Abstract: Bt cry1Ah gene which is a novel gene cloned by Chinese scientist was introduced into inbred line zong31 via agrobacterium-mediated transformation. An insect-resistant transgenic event was acquired by population screening of 1 764 transgenic plants. In this report we evaluated the transgenic event's insect resistance through laboratory and field bioassay and analyzed the foreign gene expression and the agronomic traits of hybrids generations. The results indicated that the transgenic event had high resistance to Asian corn boner(Ostrinia furnacalis), and it had no significant difference in the plant height, ear position, ear length and ear width between the transgenic event and non-transgenic plant. However, there were significantly shorter bald in transgenic event and thousand kernels weight and yield per plant were significantly higher than the control. It was extremely significantly difference in transgenic plant and non-transgenic plant under conditions of infestation by artificial simulation.

Key words: Transgenic, Naize Population, Screening, Nolecular detection Insect, Resistance Agronomic traits

戴军, 李秀影, 朱莉, 汪海, 张杰, 何康来, 郎志宏, 黄大昉. 转Bt cry1Ah基因抗虫玉米的分子检测及农艺性状分析[J]. 生物技术通报, 2014, 0(5): 62-68.

Dai Jun, Li Xiuying, Zhu Li, Wang Hai, Zhang Jie, He Kanglai, Lang Zhihong, Huang Dafang. Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene[J]. Biotechnology Bulletin, 2014, 0(5): 62-68.

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