交替氧化酶在麻疯树盐胁迫响应中的作用

摘要/Abstract

摘要： 麻疯树耐贫瘠, 能在降水量少、环境恶劣的地区种植, 但麻疯树的抗逆机理至今尚未揭晓。研究了盐胁迫对麻疯树生长的影响以及交替氧化酶（AOX）在麻疯树盐胁迫响应中的作用。结果表明, 麻疯树具有较强的耐盐性, 200 mmol/L和400 mmol/L NaCl处理对麻疯树幼苗生长影响不大, 叶片叶绿素含量和细胞含水量与对照组材料相比无明显差异。1 mol/L NaCl处理时麻疯树生长减缓, 气孔开度降低, 但未表现出明显的胁迫损伤。盐胁迫条件下, AOX呼吸和AOX基因的转录水平明显上升。与对照组材料相比, AOX基因转录水平在200 mmol/L和400 mmol/L NaCl处理时上升近两倍, 而在1 mol/L NaCl处理时上升近3倍。结果表明, AOX可能在麻疯树盐胁迫应答过程中起关键作用, 与减轻植株氧化损伤有关。

关键词: 麻疯树, 交替氧化酶, 盐胁迫, 胁迫损伤

Abstract: Jatropha curcas L. is a stress-resistant oil species and can growth well in lands with low-rainfall harsh climate conditions. However, the mechanism of Jatropha curcas L. tolerance to adverse environment conditions is still unclear. In this study, the role of alternative oxidase（AOX）in Jatropha curcas L. response to salt stress was investigated. The results showed that Jatropha curcas L. was good tolerance to 200 mmol/L and 400 mmol/L NaCl stresses, and no significant differences in chlorophyll contents and water contents between treated and untreated control plants. 1 mol/L NaCl treatment obviously suppressed plant growth and leaf development, reduced leaf stomatal conductance, but no serious oxidative damages were shown. Moreover, it should be noted that AOX pathway respiration and AOX gene transcription was induced markedly under salt stresses. Compared with control plants, AOX transcription increased two-fold in response to 200 mmol/L and 400 mmol/L salt treatments, and increased three-fold under 1 mol/L NaCl treatment. Therefore, it hypothesis that AOX plays an important role in Jatropha curcas L. salt stress tolerance.

Key words: Jatropha curcas, L. Alternative oxidase, Salt stress, Oxidative damage

余璐璐, 吴阳晨, 王静静, 魏倩, 徐飞. 交替氧化酶在麻疯树盐胁迫响应中的作用[J]. 生物技术通报, 2014, 0(7): 112-118.

Yu Lulu, Wu Yangchen, Wang Jingjing, Wei Qian, Xu Fei. The Possible Role of Alternative Oxidase in Jatropha curcas L. Responses to Salt Stress[J]. Biotechnology Bulletin, 2014, 0(7): 112-118.

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