噬菌体治疗中细菌对噬菌体的抗性

摘要/Abstract

摘要： 抗生素治疗尽管有几十年有效治疗的历史, 但随着越来越多耐/抗药性细菌的出现, 细菌对抗生素的抗药性已成为一个大问题。噬菌体治疗是使用噬菌体作为抗菌剂来感染细菌株系, 它一直是人们倡导的一个很有前途的常规抗生素治疗的替代方案。然而, 由于细菌与噬菌体的协同进化中, 细菌可以通过多种机制获得对噬菌体的抗性。因此, 人们对噬菌体治疗抱有期望的同时, 也关注噬菌体治疗长时间的使用之后, 是否会与抗生素使用之后结果相类似, 导致抗性细菌病原菌感染的治疗困难。综述了细菌-噬菌体协同进化中细菌病原菌对有感染能力的噬菌体是否会产生抗性, 及其在噬菌体治疗中影响的争论, 并展望了噬菌体治疗的潜在前景。

关键词: 细菌, 噬菌体, 噬菌体治疗, 抗性, 协同进化

Abstract: Bacterial pathogens have been treated effectively with antibiotics for several decades, but with more and more resistant bacteria appeared, the resistance to antibiotics has become a difficulty issue in antibiotics therapies. Phages therapy, the application of the phages that infect bacterial pathogens as antimicrobials, has been expected and advocated as a promising alternative to conventional antibiotics. However, under the selection of the co-evolution between phages and bacteria, bacterial pathogens can became resistance to the phages by several mechanisms. So, at the same expecting and advocating, should we worry about the problem that whether the phage-treated pathogens will develop to resistance to the phages caused to treat difficulty at last? Here, the arguments about the bacterial pathogens resistance to the phages under the phage-bacterium co-evolution and its effect on the phages therapy were summarized and reviewed, and the phage therapy for control the bacterial pathogen was prospected.

Key words: Bacterium, Phage, Phage therapy, Resistance, Co-evolution

蔡刘体, 陈兴江, 刘艳霞, 石俊雄. 噬菌体治疗中细菌对噬菌体的抗性[J]. 生物技术通报, 2014, 0(7): 33-36.

Cai Liuti, Chen Xingjiang, Liu Yanxia, Shi Junxiong. Bacterail Pathogens Resistance to Phages During the Phage Therapy[J]. Biotechnology Bulletin, 2014, 0(7): 33-36.

参考文献

[1] Andersson DI, Hughes D. Evolution of antibiotic resistance at non-lethal drug concentrations[J]. Drug Resist Update, 2012（15）：162-172.
[2] Bennett PM. Plasmid encoded antibiotic resistance：acquisition and transfer of antibiotic resistance genes in bacteria[J]. Br J Pharmacol, 2008, 153（S1）：S347-357.
[3] Jalasvuori M, Friman VP, Nieminen A, et al. Bacteriophage selection against a plasmid-encoded sex apparatus leads to the loss of antibiotic-resistance plasmids[J]. Biol Lett, 2011（7）：902-905.
[4] Dahlberg C, Chao L. Amelioration of the cost of conjugative plasmid carriage in Eschericha coli K12[J]. Genetics, 2003（165）：1641-1649.
[5] 赵庆友, 朱瑞良. 噬菌体制剂的研究现状及发展前景[J].中国兽药杂志, 2010, 44（7）：40-43.
[6] 胡重怡, 蔡刘体. 噬菌体治疗作物细菌性病害的研究进展[J]. 贵州农业科学, 2011, 39（3）：101-103.
[7] Guenther S, Huwyler D, Richard S, et al. Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods[J].Appl Environ Microbiol, 2009, 75（1）：93-100.
[8] US. Food and Drug Administration, 2007. Agency response letter GRAS notice No. GRN 000218, viewed 11/09/2007［EB/OL] . http：//www.cfsan.fda.gov/~rdb/opa-g218.html.
[9] 陈灿煌, 赵霞. 细菌对噬菌体的抵抗机制研究进展[J]. 重庆医学, 2013, 42（6）：697-700.
[10] 李铁民, 杜波. CRISPR-Cas 系统与细菌和噬菌体的共进化[J].遗传, 2011, 33（3）：213-218.
[11] Hyman P, Abedon ST. Bacteriophage host range and bacterial resistance[J]. Adv Appl Microbiol, 2010（70）：217-248.
[12] Gu J, Liu X, Li Y, et al. A method for generation phage cocktail with great therapeutic potential[J]. PLoS One, 2012（7）：e31698.
[13] Chan BK, Abedon ST. Phage therapy pharmacology phage cocktails[J]. Adv Appl Microbiol, 2012（78）：1-23.
[14] Bush K, Courvalin P, Dantas G, et al. Tackling antibiotic resistance[J]. Nat Rev Microbiol, 2011（9）：894-906.
[15] Krylov V, Shaburova O, Krylov S, Pleteneva E. A genetic approach to the development of new therapeutic phages to fight pseudomonas aeruginosa in wound infections[J]. Viruses, 2013（5）：15-53.
[16] Clokie MR, Millard AD, Letarov AV, Heaphy S. Phages in nature[J]. Bacteriophage, 2011（1）：31-45.
[17] Sechter I, Mestre F, Hansen DS. Twenty-three years of Klebsiella phage typing：a review of phage typing of 12 clusters of nosocomial infections, and a comparison of phage typing with K serotyping [J]. Clin Microbiol Infect, 2000（6）：233-238.
[18] Dennehy JJ. What can phages tell us about host-pathogen Co-evolution? [J]. Int J Evol Biol, 2012（2012）：1-13.
[19] Marston MF, Pierciey FJ Jr, Shepard A, et al. Rapid diversification of coevolving marine Synechococcus and a virus[J]. Proc Natl Acad Sci USA, 2012（109）：4544-4549.
[20] Capparelli R, Nocerino N, Iannaccone M, et al. Bacteriophage therapy of Salmonella enterica：a fresh appraisal of bacteriophage therapy[J]. J Infect Dis, 2010（201）：52-61.
[21] Orm?l? AM, Jalasvuori M. Phage therapy：should bacterial resistance to phages be a concern, even in the long run? [J].Bacteriophage, 2013, 3（1）：e24219.
[22] Gómez P, Buckling A. Bacteria-phage antagonistic coevolution in soil[J]. Science, 2011（332）：106-109.
[23] Meyer JR, Agrawal AA, Quick RT, et al. Parallel changes in host resistance to viral infection during 45, 000 generations of relaxed selection[J]. Evolution, 2010（64）：3024-3034.
[24] Inal JM. Phage therapy：a reappraisal of bacteriophages as antibiotics[J]. Arch Immunol Ther Exp（Warsz）, 2003（51）：237-244.
[25] Capparelli R, Nocerino N, Lanzetta R, et al. Bacteriophage resistant Staphylococcus aureus mutant confers broad immunity against staphylococcal infection in mice [J]. PLoS One, 2010（5）：e11720.
[26] Filippov AA, Sergueev KV, He Y, et al. Bacteriophage-resistant mutants in Yersinia pestis：identification of phage receptors and attenuation for mice[J]. PLoS One, 2011（6）：e25486.
[27] Wei Y, Ocampo P, Levin BR. An experimental study of the population and evolutionary dynamics of Vibrio cholerae O1 and the bacteriophage JSF4[J]. Proc Biol Sci, 2010（277）：3247-3254.
[28] Flores CO, Meyer JR, Valverde S, et al. Statistical structure of host-phage interactions[J]. Proc Natl Acad Sci USA, 2011（108）：E288-297.
[29] Wolf A, Wiese J, Jost G, Witzel KP. Wide geographic distribution of bacteriophages that lyse the same indigenous freshwater isolate（Sphingomonas sp. strain B18）[J]. Appl Environ Microbiol, 2003（69）：2395-2398.
[30] Sulakvelidze A, Alavidze Z, Morris JG Jr. Bacteriophage therapy[J]. Antimicrob Agents Chemother, 2001（45）：649-659.
[31] Denomy JB, Pawluk A, Maxwell KL, Davidson AR. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system[J]. Nature, 2013（493）：429-432.
[32] Seed KD, Lazinski DW, Calderwood SB, Camilli A. A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity[J]. Nature, 2013（494）：489-491.