玉米JAZ家族基因ZmJAZ4的克隆及功能分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.013

生物技术通报 ›› 2015, Vol. 31 ›› Issue (3): 96-101.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.013

玉米JAZ家族基因ZmJAZ4的克隆及功能分析

晏胜伟, 孙程, 周晓今, 陈茹梅

(中国农业科学院生物技术研究所，北京 100081)

收稿日期:2014-10-13 出版日期:2015-03-16 发布日期:2015-03-16
作者简介:晏胜伟，男，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：zhongdayan109@163.com
基金资助:
国家高技术研究发展计划“863”项目(2012AA10A306)，国家重点基础研究发展计划“973”项目(2014CB138200)，转基因专项(2008ZX08003-002)

Cloning and Characterization Analysis of ZmJAZ4，a JAZ Family Gene in Maize(Zea mays L.)

Yan Shengwei Sun Cheng Zhou Xiaojin Chen Rumei

(Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081)

Received:2014-10-13 Published:2015-03-16 Online:2015-03-16

摘要/Abstract

摘要： JAZ(Jasmonate ZIM-domain)蛋白是植物特有的一类转录因子，通过抑制茉莉素调控基因的表达，在植物的生长发育及非生物胁迫等方面发挥重要的功能。从玉米B73自交系中克隆到一个新的JAZ家族基因ZmJAZ4，该基因cDNA全长为651 bp，编码蛋白含有216个氨基酸，分子量约为23.1 kD，pI为10.78，属于碱性蛋白。Real-time RT-PCR结果表明，ZmJAZ4主要在茎端分生组织、雄穗、发育早期的种子以及胚乳中表达。系统进化分析显示，ZmJAZ4与AtJAZ10转录因子相似性较高。亚细胞定位试验表明，ZmJAZ4定位于细胞核内。ZmJAZ4在酵母细胞中不具有转录激活活性。激素及胁迫处理表明，ZmJAZ4在地上部的表达受PEG、NaCl、SA、GA和ABA诱导，而在地下部的表达受到ABA和GA诱导。结果分析表明，ZmJAZ4可能是一个重要的转录调控因子，参与调控多种激素信号通路及非生物胁迫响应。

关键词: 茉莉素, ZmJAZ基因, 逆境胁迫

Abstract: Jasmonate ZIM-domain(JAZ)proteins are a group of plant-specific transcription factors, which have important function in plant growing development and abiotic stress by inhibiting the expression of genes associated with JA regulation. In this study, a novel JAZ family gene, named ZmJAZ4, was isolated from maize(Zea mays L.)inbred lines B73. The ZmJAZ4 cDNA has a total length of 651 bp and encodes a protein of 216 amino acids with a molecular weight of about 23.1 kD and a isoelectric points of 10.78, belonging to the basic protein. Real-time RT-PCR showed that ZmJAZ4 was mainly expressed in shoot apex meristem, tassel, developing seeds and endosperm. Phylogenetic analysis revealed that ZmJAZ4 was related to AtJAZ10 from Arabidopsis. Subcellular localization experiment showed that ZmJAZ4 was localized to the nucleus. ZmJAZ4 possessed no transcriptional activating activity in yeast cells. Various treatments were performed to detect the expression level of ZmJAZ4 in response to phytohormones or abiotic stresses. The transcripts of ZmJAZ4 was induced by PEG, NaCl, SA, GA and ABA treatment in shoots and that was induced by ABA and GA in roots. The above results showed that ZmJAZ4 may be an important transcriptional regulator, which participates in many hormones signaling pathways and abiotic stress.

Key words: Jasmonate, Zea mays Jasmonate ZIM-domain gene, Abiotic stress

晏胜伟, 孙程, 周晓今, 陈茹梅. 玉米JAZ家族基因ZmJAZ4的克隆及功能分析[J]. 生物技术通报, 2015, 31(3): 96-101.

Yan Shengwei, Sun Cheng, Zhou Xiaojin, Chen Rumei. Cloning and Characterization Analysis of ZmJAZ4，a JAZ Family Gene in Maize(Zea mays L.)[J]. Biotechnology Bulletin, 2015, 31(3): 96-101.

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玉米JAZ家族基因ZmJAZ4的克隆及功能分析

Cloning and Characterization Analysis of ZmJAZ4，a JAZ Family Gene in Maize(Zea mays L.)

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