Leptin介导AMPK信号通路对猪皮下前脂肪细胞脂类代谢调控的研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.029

生物技术通报 ›› 2016, Vol. 32 ›› Issue (2): 211-218.doi: 10.13560/j.cnki.biotech.bull.1985.2016.02.029

Leptin介导AMPK信号通路对猪皮下前脂肪细胞脂类代谢调控的研究

黄英¹, 杨明华¹, 孔令富², 郝美林¹, 高士争¹, 李永能³, 赵素梅¹

1.云南农业大学云南省动物营养与饲料重点实验室，昆明 650201；2.云南农业大学动物科学技术学院，昆明 650201；3.云南农业大学招生就业处，昆明 650201

收稿日期:2015-05-05 出版日期:2016-02-24 发布日期:2016-02-25
作者简介:黄英，女，硕士，副教授，研究方向：动物分子营养与代谢调控；E-mail：hying5@sina.com
基金资助:
云南省教育厅科学研究基金项目(A3007017)，云南农业大学动物科学技术学院科学研究基金项目(DKY3P201105)

The Regulation of Leptin-mediated AMPK Signal Pathway on Lipid Metabolism in Porcine Subcutaneous Preadipocyte

HUANG Ying¹, YANG Ming-hua¹, KONG Ling-fu², HAO Mei-lin¹, GAO Shi-zheng¹, LI Yong-neng³, ZHAO Su-mei¹

(1.Yunnan Key Laboratory of Animal Nutrition and Feed，Yunnan Agricultural University，Kunming 650201；2.Faculty of Animal Science and Technology，Yunnan Agricultural University，Kunming 650201；3.Admission and Employment Office，Yunnan Agricultural University，Kunming 650201)

Received:2015-05-05 Published:2016-02-24 Online:2016-02-25

摘要/Abstract

摘要： 以体外培养的猪原代皮下脂肪细胞为研究材料，检测Leptin介导AMPK信号通路中基因表达水平，旨在阐明Leptin介导AMPK信号通路对脂肪代谢调控的分子机制。用0和100 ng/mL Leptin分别处理脂肪细胞48 h，油红O染色鉴定脂肪细胞，试剂盒测定细胞中甘油三酯和游离脂肪酸含量，Real-time PCR方法检测皮下前脂肪细胞中AMPK信号通路中基因表达水平。研究结果表明，皮下前脂肪细胞中，Leptin组甘油三酯含量均显著低于对照组(P<0.05)，表明Leptin可以降低细胞中甘油三酯和游离脂肪酸含量。Leptin组中AMPK信号通路中的基因lepR、AMPK、CPT-1基因的表达量均显著高于对照组(P<0.05)，ACC基因表达量显著低于对照组(P<0.05)，表明Leptin通过调控AMPK信号通路中基因表达，启动AMPK信号通路的信号传递。皮下前脂肪细胞中，lepR、AMPK和CPT-1基因表达量均与甘油三酯和游离脂肪酸含量呈显著负相关(P<0.05)；ACC基因表达量与甘油三酯和游离脂肪酸含量呈显著正相关(P<0.05)。结果表明，Leptin通过激活AMPK信号通路，降低ACC基因的表达量，提高CPT-1基因表达水平，促进甘油三酯分解及脂肪酸的氧化，降低细胞中甘油三酯和游离脂肪酸的含量。

关键词: 前皮下脂肪细胞, 瘦素, AMPK, 信号通路

Abstract: Using in vitro cultured porcine subcutaneous preadipocytes as the study material, the expression levels of related genes were measured for investigating the molecular regulation mechanism of leptin-mediated AMPK signal pathway on lipid metabolism.The subcutaneous adipocytes were treated with 0 and 100 ng/mL leptin for 48 h, and the adipocytes were identified by oil red O dyeing, and the contents of triglyceride and free fatty acids in the cells were measured by kit, and the mRNA expression levels of related genes in the AMPK signal pathway of subcutaneous preadipocytes were detected using Real-time PCR technology.The results showed that in leptin group, the content of the triglyceride of the subcutaneous preadipocytes was significantly lower(P<0.05)compared with the control group, indicating that the leptin reduced the contents of triglyceride and free fatty acids in the cells.The expression levels of gene lepR, AMPK, CPT-1 in AMPK signal pathway were significantly higher in leptin group than control group(P<0.05), while the expression level of gene ACC in leptin group was significantly lower than control group(P<0.05), implying that leptin initiated the signal transmission in the AMPK signal pathway by regulating the mRNA expression of related genes.The expression levels of gene lepR, AMPK, and CPT-1 were significantly negatively correlated with the content of triglyceride and free fatty acid(P<0.05)；however, the expression level of gene ACC was significantly positively correlated with the content of triglyceride and free fatty acid(P<0.05).In conclusion, the results suggested that leptin activated AMPK signal pathway, reduced the expression of ACC, and increased the expression of CPT-1, which therefore promoted the decomposition of triglyceride and oxidation of fatty acid, and finally decreased the contents of the triglycerides and free fatty acid in the cells.

Key words: subcutaneous preadipocyte, leptin, AMPK, signal pathway

黄英, 杨明华, 孔令富, 郝美林, 高士争, 李永能, 赵素梅. Leptin介导AMPK信号通路对猪皮下前脂肪细胞脂类代谢调控的研究[J]. 生物技术通报, 2016, 32(2): 211-218.

HUANG Ying, YANG Ming-hua, KONG Ling-fu, HAO Mei-lin, GAO Shi-zheng, LI Yong-neng, ZHAO Su-mei. The Regulation of Leptin-mediated AMPK Signal Pathway on Lipid Metabolism in Porcine Subcutaneous Preadipocyte[J]. Biotechnology Bulletin, 2016, 32(2): 211-218.

参考文献

[1] Luo Z, Zang M, Guo W.AMPK as a metabolic rumor suppressor：control of metabolism and cell growth[J].Future Oncol, 2010, 6(3)：457-470.
[2]Tauchi K, Ozek S, Houjou T, et al.The surface of lipid droplets is a phospholipid monolayer with a unique fatty acid composition[J]. Biological Chemistry, 2002, 277：44507-44512.
[3] 赵素梅.两种肉鸡胚胎期脂肪代谢及DHEA调控研究[D].南京：南京农业大学, 2007.
[4]Eikelis N, Lambert G, Wiesner G, et al.Extra-adipocyte Leptin release in human obesity and its relation to sympathoadrenal function[J].Am J Physiol, 2004, 286(5)：E744-E752.
[5] Nogalska A, Swierczynski J.Potential role of high serum Leptin con-centration in age-related decrease of fatty acid synthase gene expre-ssion in rat white adipose tissue[J].Experimental Gerontology, 2004, 39：147-150.
[6] Gregory R, Arend B, David J, et al.Fatty acid oxidation and triacylg-lycerol hydrolysis are enhanced after chronic Leptin treatment in Rats[J].American Journal of Physiology Endocrinology and Metablism, 2002, 282：593-600.
[7]Ramsay TG, Richards MP.Leptin and Leptin receptor expression in skeletal muscle and adipose tissue in response to in vivo porcine somatotropin treated[J].Animal Science, 2005, 83：2501-2508.
[8]Borja G, Alfredo S, Teresa F, et al.Leptin receptors in human skeletal muscle[J].Applied Physiology, 2007, 102：1786-1792.
[9]龙璐, 王平芳.LKB1-AMPK信号通路与胰岛素抵抗[J].中国糖尿病杂志, 2007, 15：382-384.
[10]Song P, Xie ZL, Wu Y, et al.Protein kinase Cζ-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells[J].Biological Chemistry, 2008, 283：12446-12455.
[11]Eijiro Y, Jeffrey E, Irwin J, et al.Fyn-dependent regulation of energy expenditure and body weight is mediated by tyrosine phosphorylation of LKB1[J].Cellular Metabolism, 2010, 11：113-124.
[12]Chung T, Blerina K, Marta K.AMPK as a mediator of hormonal signalling[J].Molecular Endocinology, 2010, 44：87-97.
[13]Li J, Grigoryev DN, Ye SQ, et al.Chronic interm ittent hypoxia upregulates genes of lipid biosynthesis in obesemice[J].Applied Physiology, 2005, 99：1643-1648.
[14]Niang F, Benelli C, Ribiere C, et al.Leptin induces nitric oxide-mediated inhibition of lipolysis and glyceroneogenesis in rat white adipose tissue[J].Nutrition, 2011, 141：4-9.
[15]Jeukendrup AE.Regulation of fat metabolism in skeletal muscle[J].Annals of the New York Academy of Sciences, 2002, 967：217-235.
[16]Knafo L, Chessex P, Rouleau T.Association between hydrogen peroxide-dependent byproducts of ascorbic acid and increased hepatic acetyl-CoA carboxylase activity[J].Clinical Chemistry, 2005, 51：1462-1471.
[17]张芳林.肉碱棕榈酰转移酶-Ⅰ的研究进展[J].国外医学内分泌学分册, 2002, 22：166-169.
[18] 符庆瑛, 高钰琪.蛋白激酶AMPK的研究进展[J].生命科学, 2005, 17：147-152.
[19]Muoio D, Seefeld K, Witters L, et al.AMP-activated kinase reciprocally regulates triacylglycerol synthesis and fatty acid oxidation in liver and muscle：evidence that sn-glycerol-3-phosphate acyltransferase is a novel target[J].Biochemistry, 1999, 338：783-791.

Leptin介导AMPK信号通路对猪皮下前脂肪细胞脂类代谢调控的研究

The Regulation of Leptin-mediated AMPK Signal Pathway on Lipid Metabolism in Porcine Subcutaneous Preadipocyte

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	丁丽, 都婷婷, 唐琼英, 高权新, 易少奎, 杨国梁. 罗氏沼虾蜕皮周期中内分泌调控和蜕皮信号通路相关基因的表达分析[J]. 生物技术通报, 2023, 39(9): 300-310.
[2]	孙雨桐, 刘德帅, 齐迅, 冯美, 黄栩筝, 姚文孔. 茉莉酸调控植物生长发育和胁迫的研究进展[J]. 生物技术通报, 2023, 39(11): 99-109.
[3]	邹晨辰, 阮灵伟, 施泓. Wnt信号通路与无脊椎动物天然免疫[J]. 生物技术通报, 2021, 37(5): 182-196.
[4]	查星琴, 杨明华, 李永能, 赵素梅, 黄英. Leptin介导JAK/STAT信号通路对猪皮下前脂肪细胞脂类代谢调控的研究[J]. 生物技术通报, 2021, 37(2): 88-95.
[5]	胡启超, 罗仍卓么, 魏大为, 杨箭, 贾立, 王兴平, 马云. 固有免疫相关编码基因在奶牛乳腺炎调节中的研究进展[J]. 生物技术通报, 2020, 36(12): 239-246.
[6]	崔荣秀, 张议文, 陈晓倩, 谷彩红, 张荃. 植物bZIP参与胁迫应答调控的最新研究进展[J]. 生物技术通报, 2019, 35(2): 143-155.
[7]	明鹏飞, 黄莹莹, 董妍丽, 聂星灿, 冯士彬, 王希春, 程建波, 李锦春, 吴金节, 李玉. LKB1-AMPKα-SIRT1信号通路在奶牛脂肪组织脂代谢中的调控作用[J]. 生物技术通报, 2019, 35(2): 176-181.
[8]	郑伟刚, 靳明辉, SwapanChakrabarty, 肖彬, 萧玉涛, 袁海滨. 小地老虎不同组织表达差异揭示其翅发育机制[J]. 生物技术通报, 2019, 35(10): 102-110.
[9]	李妙, 周立军. TRAF6与肿瘤关系的研究进展[J]. 生物技术通报, 2017, 33(6): 24-31.
[10]	李安毅, 杨洋, 刘莹, 郭晓汐, 郝倩, 徐天瑞, 安输. A-Raf-S214C突变体的构建、表达及其对MAPK信号通路的影响[J]. 生物技术通报, 2017, 33(12): 185-190.
[11]	马石楠, 李忠耀, 郭兴荣. ANGPTL8调控糖代谢作用机制研究[J]. 生物技术通报, 2017, 33(11): 200-206.
[12]	王琪, 齐仁立, 王敬, 黄金秀. 氨基酸缺乏诱导细胞自噬及miRNA调控机制[J]. 生物技术通报, 2016, 32(9): 38-43.
[13]	杨梦思,周娜,王志钢,郝慧芳. 转录因子HIF-1α及其信号通路在疾病发生中的作用研究进展[J]. 生物技术通报, 2016, 32(8): 8-13.
[14]	贾晓阳, 付玉, 王潇楠, 王志钢, 郝慧芳. 黏着斑激酶信号通路在细菌侵入非吞噬细胞中的作用[J]. 生物技术通报, 2016, 32(7): 28-33.
[15]	何丽番,高海. Mecp2在斑马鱼胚胎神经发育中对NOTCH信号通路的调控[J]. 生物技术通报, 2016, 32(4): 228-233.