小地老虎不同组织表达差异揭示其翅发育机制

doi:10.13560/j.cnki.biotech.bull.1985.2019-0336

生物技术通报 ›› 2019, Vol. 35 ›› Issue (10): 102-110.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0336

小地老虎不同组织表达差异揭示其翅发育机制

郑伟刚^{1, 2}, 靳明辉², SwapanChakrabarty², 肖彬², 萧玉涛², 袁海滨¹

1. 吉林农业大学农学院,长春 130118;
2. 中国农业科学院农业基因组研究所,深圳 518120

收稿日期:2019-04-23 出版日期:2019-10-26 发布日期:2019-09-30
作者简介:郑伟刚,硕士,研究方向：有害生物综合治理;E-mail：1126193811@qq.com
基金资助:
国家自然科学基金项目（31601646）,大鹏新区产业发展专项资金项目（KY20180216）

Difference Expression Analysis in Different Tissues of Agrotis ipsilon Reveals the Possible Mechanism of Wing Development

ZHENG Wei-gang^{1, 2}, JIN Ming-hui², SWAPAN Chakrabarty², XIAO Bin², XIAO Yu-tao², YUAN Hai-bin¹

1. College of Agriculture,Jilin Agricultural University,Changchun 130118;
2. Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120

Received:2019-04-23 Published:2019-10-26 Online:2019-09-30

摘要/Abstract

摘要： 小地老虎是典型的迁飞性农业害虫,翅发育在昆虫迁飞行为形成过程中具有重要作用,其具体的控制基因鉴定及功能分析有待深入研究。以小地老虎为材料,对其幼虫整虫,成虫头部、胸部和腹部进行转录组测序并组装。最终组装得到转录组243.4 Mb,N50长度668 bp;共组装出转录本429 288个,独立基因37 744个;KEGG数据库注释通路373个。Wnt信号通路是昆虫翅发育的关键信号通路,小地老虎Wnt信号相关通路中CaN、Frizzled、Siah-1等53个元件被注释出,小地老虎胸部组织与头部组织和小地老虎幼虫组织相比,CaN、Frizzled、Siah-1相关基因都上调表达,与腹部组织相比,CaN、Notum、Siah-1相关基因都上调表达。以上这些基因调控可能与小地老虎肌肉发育及翅的形态建成有关。

关键词: 小地老虎, Wnt信号通路, 转录组

Abstract: Black cutworm（Agrotis ipsilon）is a typical migratory agricultural pest,its wing development plays an important role in the insect migration process,and the identification and functional analysis of its specific control genes need to be further studied. The transcriptome from black cutworm whole larvae,adult head,chest,and abdomen transcriptome were sequenced and assembled. The assembled transcriptome size was 243.4 Mb and N50 contig size was 668 bp. A total of 429 288 transcripts,37 744 independent genes and 373 KEGG database annotation pathways were assembled. Wnt signaling pathways are the key ones for insect wing development. In Wnt signaling pathways in the black cutworm transcriptome,53 elements for example CaN,Frizzled and Siah-1 were annotated. CaN,Frizzled and Siah-1 in the thoracic tissues were up-regulated compared to the head and larval tissues;while CaN,Notum and Siah-1 in the thoracic tissues were up-regulated compared to the abdominal tissues. In sum,these genes regulation may be related to the muscle development and wings morphogenesis of the black cutworm.

Key words: Agrotis ipsilon, Wnt signaling pathway, transcriptome

郑伟刚, 靳明辉, SwapanChakrabarty, 肖彬, 萧玉涛, 袁海滨. 小地老虎不同组织表达差异揭示其翅发育机制[J]. 生物技术通报, 2019, 35(10): 102-110.

ZHENG Wei-gang, JIN Ming-hui, SWAPAN Chakrabarty, XIAO Bin, XIAO Yu-tao, YUAN Hai-bin. Difference Expression Analysis in Different Tissues of Agrotis ipsilon Reveals the Possible Mechanism of Wing Development[J]. Biotechnology Bulletin, 2019, 35(10): 102-110.

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小地老虎不同组织表达差异揭示其翅发育机制

Difference Expression Analysis in Different Tissues of Agrotis ipsilon Reveals the Possible Mechanism of Wing Development

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