盐度对一株淡水栅藻Scenedesmus sp.生长及生化组成的影响

doi:10.13560/j.cnki.biotech.bull.1985.2016-1159

生物技术通报 ›› 2017, Vol. 33 ›› Issue (7): 155-161.doi: 10.13560/j.cnki.biotech.bull.1985.2016-1159

盐度对一株淡水栅藻Scenedesmus sp.生长及生化组成的影响

李嘉颖¹,²,李涛¹,谭丽¹,²,吴嘉仪¹,向文洲¹,刘德海³

1. 中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室，广州 510301；
2. 中国科学院大学，北京 100049；
3. 广州市白云联佳精细化工厂，广州 510412

收稿日期:2016-12-22 出版日期:2017-07-11 发布日期:2017-07-11
作者简介:李嘉颖，女，硕士研究生，研究方向：微藻生物技术；E-mail：kar_in@163.com
基金资助:
广东省海洋渔业科技攻关与研发项目（A201601A13），中国科学院可再生能源重点实验室基金（Y607k91001、Y507jb1001），广东丹姿集团科技研发项目（DZ201501）

Effects of Salinity on the Growth and Biochemical Properties of a Freshwater Algae Scenedesmus sp.

LI Jia-ying¹,² ,LI Tao¹, TAN Li¹,² ,WU Jia-yi¹ ,XIANG Wen-zhou¹, LIU De-hai³

1. Key Laboratory of Tropical Marine Bio-resources and Ecology，South China Sea Institute of Oceanology，Chinese Academy of Sciences，Guangzhou 510301；
2. University of Chinese Academy of Sciences，Beijing 100049；
3. Guangzhou Baiyun Lianjia Fine Chemical Factory，Guangzhou 510412

Received:2016-12-22 Published:2017-07-11 Online:2017-07-11

摘要/Abstract

摘要： 通过管式光生物反应器培养，分析比较不同盐度（0-40‰）对一株淡水栅藻（Scenedesmus sp.）生长和生化特性影响，评价海水培养该藻株的产业化潜力，探讨其高盐适应机制，为进一步海水驯化研究提供理论参考。采用显微观察、索氏提取法、凯氏定氮法、苯酚硫酸法、气相色谱法及高效液相色谱法分别测定微藻生长及蛋白质、油脂、多糖含量以及色素、脂肪酸组成情况。结果表明，高盐条件下藻细胞明显增大，并出现自然沉降现象，可实现低成本采收。随盐度增加，栅藻生长逐步被抑制，40‰盐度条件下生长完全停止，但在3‰盐度下仍良好生长，培养末期生物量可达2.84 g/L。30‰盐度组蛋白质含量相对淡水组提高95.40%、产率与淡水组相近，加之高盐培养可较大幅度降低蛋白质的生产成本，因此，采用海水培养栅藻来开发蛋白资源具有一定的潜力。研究还表明，30‰盐度下藻细胞内β-胡萝卜素、虾青素、可溶性多糖等成分大量积累，可能是其适应高盐胁迫的重要生理基础。

关键词: 淡水栅藻, Scenedesmus sp., 高盐胁迫, 生长, 生化组成

Abstract: The aims of this work are to analyze and compare the effects of different salt concentration（0-40‰）on the growth and biochemical characteristics of a freshwater algae Scenedesmus sp. incubated in small tubular bioreactors，and evaluate the industrialization potential of culturing this algae in seawater as well explore the adaptive mechanism at high salinity，which provides theoretical references for further study on seawater domestication. The growth of the microalgae，the content of protein，lipid and polysaccharide，and the composition of pigments and fatty acids were analyzed correspondingly by microscopic observation，Soxhlet extraction，Kjeldahl method，phenol sulfuric acid determination，gas chromatography technology，and HPLC. The results showed that cells grew bigger and naturally precipitated at high salinity，meaning that it can be harvested at low cost. The study also found that growth of Scenedesmus sp. was gradually inhibited with the increase of salinity，and completely inhibited at the salinity of 40‰；however，it still grew well at the salinity of 30‰ with biomass of 2.84 g/L，and higher protein content（95.40% higher than 0‰ group）with similar protein productivity as that by freshwater. Combined with the great cost reduction of producing protein at high salinity，culturing Scenedesmus sp. in seawater for protein resource is of high potential. Moreover，with the increase of salinity，Scenedesmus sp. tended to accumulate more β-carotene，astaxanthin，and even soluble sugar，which might be the key physiological basis of adapting salt-stress.

Key words: freshwater microalgae, Scenedesmus sp., high-salinity stress, growth, biochemical composition

李嘉颖,李涛,谭丽,吴嘉仪,向文洲,刘德海. 盐度对一株淡水栅藻Scenedesmus sp.生长及生化组成的影响[J]. 生物技术通报, 2017, 33(7): 155-161.

LI Jia-ying,LI Tao, TAN Li,WU Jia-yi ,XIANG Wen-zhou, LIU De-hai. Effects of Salinity on the Growth and Biochemical Properties of a Freshwater Algae Scenedesmus sp.[J]. Biotechnology Bulletin, 2017, 33(7): 155-161.

参考文献

[1] Tang YT, Rosenberg JN, Bohutskyi P, et al. Microalgae as a feedstock for biofuel precursors and value-added products：green fuels and golden opportunities[J] . BioResources, 2016, 11：36.
[2] Spolaore P, Joannis-Cassan C, Duran E, et al. Commercial applications of microalgae[J] . Journal of Bioscience and Bioengineering, 2006, 101：87-96.
[3] Sibi G, Shetty V, Mokashi K. Enhanced lipid productivity approaches in microalgae as an alternate for fossil fuels-A review[J] . J Energy Inst, 2016, 89：330-334.
[4] Minhas AK, Hodgson P, Barrow CJ, et al. A Review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids[J] . Front Microbiol, 2016, 7：19.
[5] Rao AR, Dayananda C, Sarada R, et al. Effect of salinity on growth of green alga Botryococcus braunii and its constituents[J] . Bioresource Technology, 2007, 98：560-564.
[6] Fazeli MR, Tofighi H, Samadi N, et al. Effects of salinity on beta-carotene production by Dunaliella tertiolecta DCCBC26 isolated from the Urmia salt lake, north of Iran[J] . Bioresource Technology, 2006, 97：2453-2456.
[7] Kim BH, Ramanan R, Kang Z, et al. Chlorella sorokiniana HS1, a novel freshwater green algal strain, grows and hyperaccumulates lipid droplets in seawater salinity[J] . Biomass Bioenerg, 2016, 85：300-305.
[8] Paliwal C, Pancha I, Ghosh T, et al. Selective carotenoid accumula-tion by varying nutrient media and salinity in Synechocystis sp. CCNM 2501[J] . Bioresource Technology, 2015, 197：363-368.
[9] 刘建国, 龙元薷, 黄园, 等. 微藻生物柴油研究现状与发展策略[J] . 海洋科学, 2013, 37（10）：132-141.
[10] 孙丽英, 何皓, 田宜水, 等. 微藻规模化生产的关键问题[J] . 可再生能源, 2012, 30（9）：70-74.
[11] 吴伯堂, 何汝洪, 彭云辉. 钝顶螺旋藻海水驯化的初步研究[J] . 海洋与湖沼, 1988, 19（2）：197-200.
[12] 向文洲, 李涛, 吴华莲, 等. 海水螺旋藻产业发展战略研究[J] . 广西科学, 2014（6）：573-579.
[13] Chen H, Jiang JG. Osmotic responses of Dunaliella to the changes of salinity[J] . Journal of Cellular Physiology, 2009, 219：251-258.
[14] Li T, Wan LL, Li AF, et al. Responses in growth, lipid accumula-tion, and fatty acid composition of four oleaginous microalgae to different nitrogen sources and concentrations[J] . Chin J Oceanol Limnol, 2013, 31：1306-1314.
[15] Khozin-Goldberg I, Shrestha P, Cohen Z. Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa[J] . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 2005, 1738：63-71.
[16] Christie WW. Lipid analysis：isolation, separation, identification, and structural analysis of lipids[M] ：Pergamon Press, 1982.
[17] Li T, Xu J, Gao B, et al. Morphology, growth, biochemical composition and photosynthetic performance of Chlorella vulgaris（Trebouxiophyceae）under low and high nitrogen supplies[J] . Algal Res, 2016, 16：481-491.
[18] Lichtenthaler HK. Chlorophylls and carotenoids：Pigments of photosynthetic biomembranes[M] . Method Enzymol：Academic Press, 1987：350-382.
[19] Grima EM, Belarbi EH, Fernandez FGA, et al. Recovery of microalgal biomass and metabolites：process options and economics[J] . Biotechnology Advances, 2003, 20：491-515.
[20] von Alvensleben N, Magnusson M, Heimann K. Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles[J] . Journal of Applied Phycology, 2016, 28：861-876.
[21] Oren A. Bioenergetic aspects of halophilism[J] . Microbiology and Molecular Biology Reviews, 1999, 63：334-348.
[22] 麦康森. 水产饲料的蛋白源问题[J] . 科学养鱼, 2014, 6：4.
[23] 苏小凤, 邵庆均. 多不饱和脂肪酸在鱼类营养与饲料中的作用及其氧化稳定性[J] . 饲料研究, 2002, 3：11-14.
[24] Dufosse L, Galaup P, Yaron A, et al. Microorganisms and microal-gae as sources of pigments for food use：a scientific oddity or an industrial reality?[J] . Trends in Food Science and Technology, 2005, 16（9）：389-406.
[25] Lyon BR, Bennett-Mintz JM, Lee PA, et al. Role of dimethylsulfoni-opropionate as an osmoprotectant following gradual salinity shifts in the sea-ice diatom Fragilariopsis cylindrus[J] . Environ Chem, 2016, 13：181-194.
[26] Scholz B, Liebezeit G. Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities[J] . European Journal of Phycology, 2012, 47：393-407.
[27] Talebi AF, Tabatabaei M, Mohtashami SK, et al. Comparative salt stress study on intracellular ion concentration in marine and salt-adapted freshwater strains of microalgae[J] . Notulae Scientia Biologicae, 2013, 5：309.
[28] 毕永红, 邓中洋, 胡征宇, 等. 发状念珠藻对盐胁迫的响应[J] . 水生生物学报, 2005, 29（2）：125-129
[29] 毛桂莲, 许兴, 徐兆桢. 植物耐盐生理生化研究进展[J] . 中国生态农业学报, 2004, 12（1）：43-46.
[30] Sarada R, Tripathi U, Ravishankar GA. Influence of stress on astaxanthin production in Haematococcus pluvialis grown under different culture conditions[J] . Process Biochem, 2002, 37：623-627.
[31] Lemoine Y, Schoefs B. Secondary ketocarotenoid astaxanthin biosynthesis in algae：a multifunctional response to stress[J] . Photosynthesis Research, 2010, 106：155-177.

盐度对一株淡水栅藻Scenedesmus sp.生长及生化组成的影响

Effects of Salinity on the Growth and Biochemical Properties of a Freshwater Algae Scenedesmus sp.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	周嫒婷, 彭睿琦, 王芳, 伍建榕, 马焕成. 生防菌株DZY6715在不同生长期的代谢差异分析[J]. 生物技术通报, 2023, 39(9): 225-235.
[2]	杨玉梅, 张坤晓. 应用CRISPR/Cas9技术建立ERK激酶相分离荧光探针定点整合的稳定细胞株[J]. 生物技术通报, 2023, 39(8): 159-164.
[3]	付钰, 贾瑞瑞, 何荷, 王良桂, 杨秀莲. 两种砧木楸树嫁接苗生长差异及转录组比较分析[J]. 生物技术通报, 2023, 39(8): 251-261.
[4]	胡海琳, 徐黎, 李晓旭, 王晨璨, 梅曼, 丁文静, 赵媛媛. 小肽激素调控植物生长发育及逆境生理研究进展[J]. 生物技术通报, 2023, 39(7): 13-25.
[5]	冯珊珊, 王璐, 周益, 王幼平, 方玉洁. WOX家族基因调控植物生长发育和非生物胁迫响应的研究进展[J]. 生物技术通报, 2023, 39(5): 1-13.
[6]	刘辉, 卢扬, 叶夕苗, 周帅, 李俊, 唐健波, 陈恩发. 外源硫诱导苦荞镉胁迫响应的比较转录组学分析[J]. 生物技术通报, 2023, 39(5): 177-191.
[7]	罗义, 张丽娟, 黄伟, 王宁, 吾尔丽卡·买提哈斯木, 施宠, 王玮. 一株耐铀菌株的鉴定及其促生特性研究[J]. 生物技术通报, 2023, 39(5): 286-296.
[8]	姚姿婷, 曹雪颖, 肖雪, 李瑞芳, 韦小妹, 邹承武, 朱桂宁. 火龙果溃疡病菌实时荧光定量PCR内参基因的筛选[J]. 生物技术通报, 2023, 39(5): 92-102.
[9]	薛皦, 朱庆锋, 冯彦钊, 陈沛, 刘文华, 张爱霞, 刘勤坚, 张琪, 于洋. 植物基因上游开放阅读框的研究进展[J]. 生物技术通报, 2023, 39(4): 157-165.
[10]	魏明, 王欣玉, 伍国强, 赵萌. NAD依赖型去乙酰化酶SRT在植物表观遗传调控中的作用[J]. 生物技术通报, 2023, 39(4): 59-70.
[11]	桑田, 王鹏程. 植物SUMO化修饰研究进展[J]. 生物技术通报, 2023, 39(3): 1-12.
[12]	王海龙, 李雨倩, 王勃, 邢国芳, 张杰伟. 谷子SiMAPK3基因的克隆和表达特性分析[J]. 生物技术通报, 2023, 39(3): 123-132.
[13]	杨春洪, 董璐, 陈林, 宋丽. 大豆VAS1基因家族的鉴定及参与侧根发育的研究[J]. 生物技术通报, 2023, 39(3): 133-142.
[14]	孙雨桐, 刘德帅, 齐迅, 冯美, 黄栩筝, 姚文孔. 茉莉酸调控植物生长发育和胁迫的研究进展[J]. 生物技术通报, 2023, 39(11): 99-109.
[15]	安昌, 陆琳, 沈梦千, 陈盛圳, 叶康卓, 秦源, 郑平. 植物bHLH基因家族研究进展及在药用植物中的应用前景[J]. 生物技术通报, 2023, 39(10): 1-16.