一株聚丙烯酰胺降解菌的筛选及降解特性研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0292

生物技术通报 ›› 2019, Vol. 35 ›› Issue (9): 178-183.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0292

一株聚丙烯酰胺降解菌的筛选及降解特性研究

刘宇程¹, 邱恋¹, 梁晶晶¹, 李玲丽¹, 马丽丽^1,2

1. 西南石油大学化学化工学院,成都 610500;
2. 南开大学环境污染过程与基准教育部重点实验室,天津 300350

收稿日期:2019-04-09 出版日期:2019-09-26 发布日期:2019-09-16
作者简介:刘宇程,男,教授,研究方向：油气田污染治理;E-mail：lycswpi@163.com
基金资助:
国家自然科学基金项目（41601341）,环境污染过程与基准教育部重点实验室开放课题（2018-05）,四川省科技计划重点研发项目（2018GZ0421）

Screening and Degradation Characteristics of a Strain Degrading Polyacrylamide

LIU Yu-cheng¹, QIU Lian¹, LIANG Jing-jing¹, LI Ling-li¹, MA Li-li^1,2

1. School of Chemistry and Chemical engineering,Southwest Petroleum University,Chengdu 610500;
2. Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria,Nankai University,Tianjin 300350

Received:2019-04-09 Published:2019-09-26 Online:2019-09-16

摘要/Abstract

摘要： 从含聚丙烯酰胺（Polyacrylamide,PAM）污泥中筛分出一株聚丙烯酰胺降解菌,该菌能同时以PAM为唯一碳源和唯一氮源生长。在PAM起始浓度为1.2 g/L、温度为35℃、pH为7.0时,PCX菌株（接菌量5%）在48 h内对PAM的降解率可达43%。经生理生化和16S rDNA序列分析,确定该菌株为克雷伯氏菌属（Klebsiella sp.）,命名为PCX。聚丙烯酰胺（分子量：3×10³ kD）降解后其产物分子量为0.619 kD。结合红外光谱结果,推断其分子链上的酰胺基水解成羧基,侧链降解。生物急性毒性分析结果表明,其EC₅₀为106 mg/L,说明降解产物对水生生物无毒害作用。

关键词: 菌株鉴定, 克雷伯氏菌, 聚丙烯酰胺, 降解特性

Abstract: A bacterial strain degrading polyacrylamide was screened from the sludge containing polyacrylamide（PAM）. The bacterium can grow with polyacrylamide as the sole carbon source and nitrogen source. The degradation rate of PAM by the strain（inoculation 5%）reached 43% within 48 h when the initial concentration of PAM was 1.2 g/L,the temperature was 35℃,and the pH was 7.0. The strain was identified as Klebsiella sp.,named PCX,after physiological,biochemical and 16S rDNA sequence analysis. The molecular weight of the degraded product of PAM（molecular weight：3×10³ kD）was 0.619 kD. Combined with the results from infrared spectroscopy,the amide group on the molecular chain was hydrolyzed to carboxyl group and the side chain was degraded. The results of acute biological toxicity analysis showed that the EC₅₀ was 106 mg/L,indicating that the degraded products had no toxic effect on aquatic organisms.

Key words: strain identification, Klebsiella, polyacrylamide, degradation characteristics

刘宇程, 邱恋, 梁晶晶, 李玲丽, 马丽丽. 一株聚丙烯酰胺降解菌的筛选及降解特性研究[J]. 生物技术通报, 2019, 35(9): 178-183.

LIU Yu-cheng, QIU Lian, LIANG Jing-jing, LI Ling-li, MA Li-li. Screening and Degradation Characteristics of a Strain Degrading Polyacrylamide[J]. Biotechnology Bulletin, 2019, 35(9): 178-183.

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一株聚丙烯酰胺降解菌的筛选及降解特性研究

Screening and Degradation Characteristics of a Strain Degrading Polyacrylamide

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