生物技术通报 ›› 2020, Vol. 36 ›› Issue (11): 245-258.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1273

• 技术与方法 • 上一篇    下一篇

基于核酸适配体传感器检测食品致病菌的研究进展

王琦1(), 颜春蕾1, 高洪伟2, 吴薇1(), 杨庆利1()   

  1. 1.青岛农业大学食品科学与工程学院,青岛 266109
    2.青岛农业大学机电工程学院,青岛 266109
  • 收稿日期:2019-12-29 出版日期:2020-11-26 发布日期:2020-11-20
  • 作者简介:王琦,女,硕士研究生,研究方向:食品危害物的快速检测;E-mail: 17854231141@163.com
  • 基金资助:
    国家自然科学基金项目(81703228);国家重点研发计划项目(2016YFD0400105);中国博士后科学基金(2019M652320);山东省高等学校青创人才引育团队(2019-135)

Research Progress of DNA Aptasensors for Foodborne Pathogen Detection

WANG Qi1(), YAN Chun-lei1, GAO Hong-wei2, WU Wei1(), YANG Qing-li1()   

  1. 1. College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109
    2. Electromechanic Engineering College,Qingdao Agricultural University,Qingdao 266109
  • Received:2019-12-29 Published:2020-11-26 Online:2020-11-20

摘要:

核酸适配体是可以识别并与靶标分子结合的单链DNA或RNA分子。由于适配体具有良好的特异性、稳定性、选择性、易于修饰和亲和力高等特点,在病原检测和其他小分子识别方面具有很大的潜力。综述了适配体的筛选技术以及适配体应用技术,包括比色法、可见光谱法、荧光法、电化学法、表面增强拉曼法和胶体金试纸条法等在食源性致病菌检测中的应用。目前适配体传感器在检测食品致病菌领域取得了显著进展。然而核酸适配体应用方面的挑战和缺陷仍需克服。

关键词: 适配体, 食品安全, 快速检测, SELEX, 大肠杆菌, 沙门氏菌

Abstract:

Aptamers are single-stranded DNA or RNA molecules which possess specific identification and binding ability to their targets. Aptamers present breathtaking potential in the field of foodborne pathogen detection and other bio-molecular recognition,owing to their excellent specificity,stabilization,selectivity,simplicity of modification,and high affinity. This review outlines the technologies of aptamer screening and aptamer application,including colorimetric assays,visible spectrum assay,fluorescence assays,electrochemical assays,surface-enhanced Raman assays,and gold-nanoparticle lateral flow assays in the determination of foodborne pathogens. Until now,the remarkable progress(more rapid,sensitive,and accurate)in the monitoring of foodborne pathogen on aptasensor has been achieved;however,there are still many challenges and flaws in their utilization to be overcome.

Key words: aptamer, food safety, rapid detection, SELEX, Escherichia coli, Salmonella