葡萄ERECTA基因过表达提高拟南芥生长量和耐热性

doi:10.13560/j.cnki.biotech.bull.1985.2019-1170

生物技术通报 ›› 2020, Vol. 36 ›› Issue (6): 46-53.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1170

葡萄ERECTA基因过表达提高拟南芥生长量和耐热性

刘敏¹, 房玉林^2,3

1. 山西农业大学食品科学与工程学院,太谷 030801;
2. 西北农林科技大学葡萄酒学院,杨凌 712100;
3. 陕西省葡萄与葡萄酒工程技术研究中心,杨凌 712100

收稿日期:2019-12-03 出版日期:2020-06-26 发布日期:2020-06-28
作者简介:刘敏,女,博士,讲师,研究方向：葡萄逆境生理;E-mail：liumin272@163.com
基金资助:
国家现代农业（葡萄）产业技术体系建设专项资助项目（CARS-29-zp-06）,山西农业大学科技创新基金项目（2018YJ53）,山西省优秀博士来晋工作奖励资金科研项目（SXYBKY2019002）

Overexpression of Grape ERECTA Enhances Growth and Thermo-tolerance of Arabidopsis

LIU Min¹, FANG Yu-lin^2,3

1. College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801;
2. College of Enology,Northwest A & F University,Yangling 712100;
3. Shaanxi Engineering Research Center for Viti-Viniculture,Yangling 712100

Received:2019-12-03 Published:2020-06-26 Online:2020-06-28

摘要/Abstract

摘要： 葡萄ERECTA基因受高温诱导表达,且在葡萄的幼叶和花中表达量较高。为了研究葡萄ERECTA基因在植物生长发育和高温响应中的作用,将该基因在拟南芥中过表达,观察其表型变化和对高温的耐受性。结果表明,葡萄ERECTA基因过表达可以显著增加拟南芥叶片的长度、宽度和数量,花梗变细长,角果长度和株高显著增加。将拟南芥种子在高温处理后常温培养2 d,大部分Landsberg erecta（Ler）幼苗发育不正常,而过表达型ER-OE幼苗均未受到高温胁迫的影响。拟南芥植株在40℃高温处理4 d,ER-OE植株受胁迫程度较轻,叶片电导率的顺序为Ler>Col>ER-OE;在22℃恢复5 d后,Col、Ler和ER-OE的存活率分别为33%、12%和83%。ERECTA基因既可以提高植株的耐热性,又可以增加生长量,为培育葡萄抗性新品种提供了新的基因资源,在农业生产上有很好的应用前景。

关键词: 葡萄, ERECTA, 生长发育, 高温, 耐热性

Abstract: ERECTA gene expression in grape is induced by high temperature,and its expression level is high in young leaves and flowers. In order to study the role of grape ERECTA gene in plant growth/development and the response to high temperature,this gene was overexpressed in Arabidopsis,and its phenotype and thermo-tolerance were observed. The results showed that in the ERECTA-overexpressed Arabidopsis,the length,width and number of leaves increased,the pedicel became elongated,and pod length and plant height also increased significantly. Arabidopsis seeds were cultured at room temperature for 2 d after heat treatment. It was found that most Landsberg erecta（Ler）seedlings did not develop normally,while overexpressed seedlings ER-OE were not affected by heat stress. After Arabidopsis plants were treated at 40℃ for 4 d,ER-OE plants were subjected to little heat damage. The electrolyte leakage of leaves was Ler>Col>ER-OE. After recovery at 22℃for 5 d,the survival rate of Col,Ler and ER-OE was 33%,12% and 83%,respectively. ERECTA can not only improve the heat resistance of plants,but also increase the growth amount,which provides a new genetic resource for breeding new resistant varieties of grape,and has a promising application prospect in agricultural production.

Key words: grape, ERECTA, growth and development, high temperature, thermo-tolerance

刘敏, 房玉林. 葡萄ERECTA基因过表达提高拟南芥生长量和耐热性[J]. 生物技术通报, 2020, 36(6): 46-53.

LIU Min, FANG Yu-lin. Overexpression of Grape ERECTA Enhances Growth and Thermo-tolerance of Arabidopsis[J]. Biotechnology Bulletin, 2020, 36(6): 46-53.

参考文献

[1] Shpak ED.Diverse roles of ERECTA family genes in plant develop-ment[J]. Journal of Integrative Plant Biology, 2013, 55(12):1238-1250.
[2] Torii KU, Mitsukawa N, Oosumi T, et al.The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats[J]. Plant Cell, 1996, 8(4):735-746.
[3] Shpak ED, Mcabee JM, Pillitteri LJ, et al.Stomatal patterning and differentiation by synergistic interactions of receptor kinases[J]. Science, 2005, 309(5732):290-293.
[4] Lee JS, Kuroha T, Hnilova M, et al.Direct interaction of ligand-receptor pairs specifying stomatal patterning[J]. Genes & Development, 2012, 26(2):126-136.
[5] Meng X, Chen X, Mang H, et al.Differential function of Arabidopsis SERK family receptor-like kinases in stomatal patterning[J]. Current Biology, 2015, 25(18):2361-2372.
[6] Masle J, Gilmore SR, Farquhar GD.The ERECTA gene regulates plant transpiration efficiency in Arabidopsis[J]. Nature, 2005, 436:866-870.
[7] 朱锦程, 沈海涛, 祝建波. 拟南芥ERECTA基因的克隆及其对番茄转化[J]. 生物技术通报, 2010(8):102-105.
[8] 郭鹏, 夏新莉, 尹伟伦. 3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达[J]. 生态学报, 2011, 31(11):3239-3245.
[9] Xing HT, Guo P, Xia XL, et al.PdERECTA, a leucine-rich repeat receptor-like kinase of poplar, confers enhanced water use efficiency in Arabidopsis[J]. Planta, 2011, 234(2):229-241.
[10] Liu J, Zhang F, Zhou J, et al.Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice[J]. Plant Molecular Biology, 2012, 78(3):289-300.
[11] Blair MW, Cortés AJ, Dominique T.Identification of an ERECTA gene and its drought adaptation associations with wild and cultivated common bean[J]. Plant Science, 2016, 242:250-259.
[12] 郑甲成, 刘婷, 杜莹莹, 等. 高温胁迫下TaERECTA基因对小麦幼苗抗氧化物酶活性的影响[J]. 甘肃农业大学学报, 2018(3):70-75.
[13] 霍建飞, 刘春艳, 郝永娟, 等. 植物中重要的信号分子—CaM[J]. 天津农业科学, 2010, 16(3):30-35.
[14] 段江燕, 张俊红. 植物细胞中钙调素研究进展[J]. 农业与技术, 2009, 29(6):35-39.
[15] 高宁. 钙调素与ERECTA相互作用的研究[D]. 石家庄:河北师范大学, 2008.
[16] Liu M, Li W, Min Z, et al.Identification and expression analysis of ERECTA family genes in grape(Vitis vinifera L.)[J]. Genes & Genomics, 2019, 41(6):723-735.
[17] Jiang J, Liu X, Liu C, et al.Integrating omics and alternative splicing reveals insights into grape response to high temperature[J]. Plant Physiology, 2017, 173(2):1502-1518.
[18] Livak KJ, Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2 ^-ΔΔCT method[J]. Methods, 2001, 25:402-408.
[19] 杨敏, 韩玉珍, 阿依江·哈拜克, 等. LRR-RLKs亚家族基因RLK6在拟南芥开花过程中的作用[J]. 核农学报, 2017(4):654-662.
[20] Shpak ED, Berthiaume CT, Hill EJ, et al.Synergistic interaction of three ERECTA-family receptor-like kinases controls Arabidopsis organ growth and flower development by promoting cell proliferation[J]. Development, 2004, 131(7):1491-1501.
[21] Villagarcia H, Morin A, Shpak ED, et al.Modification of tomato growth by expression of truncated ERECTA protein from Arabidopsis thaliana[J]. Journal of Experimental Botany, 2012, 63(18):6493-6504.
[22] Shen H, Zhong X, Zhao F, et al.Overexpression of receptor-like kinase ERECTA improves thermotolerance in rice and tomato[J]. Nature Biotechnology, 2015, 33(9):996-1003.
[23] Uchida N, Lee JS, Horst RJ, et al.Regulation of inflorescence architecture by intertissue layer ligand-receptor communication between endodermis and phloem[J]. PNAS, 2012, 109:6337-6342.
[24] Meng X, Wang H, He Y, et al.A MAPK cascade downstream of ERECTA receptor-like protein kinase regulates Arabidopsis inflorescence architecture by promoting localized cell proliferation[J]. Plant Cell, 2012, 24(12):4948-4960.
[25] Nemhauser JL, Mockler TC, Chory J.Interdependency of brassinosteroid and auxin signaling in Arabidopsis[J]. PLoS Biol, 2004, 2(9):e258.
[26] Olszewski N, Sun TP, Gubler F.Gibberellin signaling:biosynthesis, catabolism, and response pathways[J]. The Plant Cell, 2002, 14(Supplement):S61-S80.
[27] Du J, Jiang H, Sun X, et al.Auxin and gibberellins are required for the receptor-like kinase ERECTA regulated hypocotyl elongation in shade avoidance in Arabidopsis[J]. Frontiers in Plant Science, 2018, 9:124.
[28] Braybrook SA, Kuhlemeier C.How a plant builds leaves[J]. The Plant Cell, 2010, 22(4):1006-1018.
[29] Kimura Y, Tasaka M, Torii KU, et al. ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem[J]. Development, 2017, 145(1):dev156380.
[30] Chen MK, Wilson RL, Palme K, et al.ERECTA family genes regulate auxin transport in the shoot apical meristem and forming leaf primordia[J]. Plant Physiology, 2013, 162(4):1978-1991.
[31] Ruelland E, Zachowski A.How plants sense temperature[J]. Environmental & Experimental Botany, 2010, 69(3):225-232.

葡萄ERECTA基因过表达提高拟南芥生长量和耐热性

Overexpression of Grape ERECTA Enhances Growth and Thermo-tolerance of Arabidopsis

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