植物非组蛋白赖氨酸乙酰化修饰的蛋白质组学研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-1080

摘要/Abstract

摘要：

蛋白质赖氨酸乙酰化是植物中普遍存在的重要蛋白质翻译后修饰过程。过去的研究主要集中在染色体组蛋白的乙酰化修饰及其调控机制。目前,随着定量乙酰化蛋白质组学技术的发展,大量非组蛋白赖氨酸乙酰化修饰被发现,其在植物中存在的普遍性及其生理功能的重要性也随之凸显。非组蛋白赖氨酸乙酰化修饰在植物不同组织、器官和细胞器中大量存在,广泛参与植物生长发育的各种代谢过程的调控,并在植物应答和适应逆境胁迫中发挥作用。综述了近年来植物非组蛋白赖氨酸乙酰化修饰的蛋白质组学研究进展,阐明乙酰化修饰在植物不同组织和亚细胞中的分布特征以及在植物生长发育和逆境胁迫响应中的作用,并阐述乙酰化修饰与其他蛋白质翻译后修饰的交互作用,最后对未来的研究进行展望和讨论。

关键词: 非组蛋白, 赖氨酸乙酰化, 乙酰化蛋白质组, 生长发育, 胁迫响应

Abstract:

Protein lysine acetylation is an important and common protein post-translational modification in plants. The prior researches had focused on acetylation modification of histones in chromosomes and their regulatory mechanisms. Recently,with the development of quantitative acetylproteome,a large number of non-histone proteins with lysine acetylation modification have been discovered,and the universality of its existence in biology and the importance of its function have also been highlighted. Non-histone protein lysine acetylation modification is abundant in different tissues,organs and organelles of plants. It is widely involved in various metabolic processes during the growth and development,and also plays important roles in responses and adaptation to stresses. In this review,we summarize the recent acetylproteome researches in non-histone lysine acetylation modification in plants. Then we describe the distribution characteristics of identified acetylated proteins in different tissues and subcellular organelles. Further we reveal the functions of acetylation modification in growth,development and responses to stresses,crosstalk between lysine acetylation and other protein post-translational modifications. Finally,we prospect and discuss the future research directions.

Key words: non-histone proteins, lysine acetylation, acetylproteome, growth and development, stress responses

郑璐, 沈仁芳, 兰平. 植物非组蛋白赖氨酸乙酰化修饰的蛋白质组学研究进展[J]. 生物技术通报, 2021, 37(1): 77-89.

ZHENG Lu, SHEN Ren-fang, LAN Ping. Research Progress of Plant Lysine Acetylproteome Modified in Non-histone Protein[J]. Biotechnology Bulletin, 2021, 37(1): 77-89.

图/表 4

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植物	组织或器官（处理条件）	实验方法	蛋白质数	位点数	主要生物过程或代谢途径	参考文献
拟南芥	悬浮细胞、地上部、线粒体、叶绿体	抗体亲和富集、LC-MS/MS	74	91	光合作用	[19]
	叶片、长角果、花和种子、根	1/2-DE、LC-MS/MS	57	64	光合作用	[20]
	线粒体	抗体亲和富集、LC-MS/MS	120	243	TCA循环、呼吸链、光呼吸、氨基酸和蛋白质代谢、氧化还原调节	[5]
	叶片（赖氨酸去乙酰化酶抑制剂）	二甲基标记、抗体亲和富集、LC-MS/MS	1 022	2 152	光合作用	[21]
	21 d幼苗的地上部,乙烯处理	梯度离心、二甲基标记、抗体亲和富集、LC-MS/MS	2 638	7 456	组蛋白超家族、核糖体、热休克、胁迫/刺激、能量代谢	[22]
	不同组织（白天结束和晚上结束时）	抗体亲和富集、LC-MS/MS	909	1 365	捕光和光合作用、翻译、代谢、细胞运输	[23]
水稻	悬浮细胞	抗体亲和富集、LC-MS/MS	44	60	胁迫响应、DNA代谢、氮代谢、生物合成	[24]
	幼苗	抗体亲和富集、LC-MS/MS	716	1 337	乙醛酸和二羧酸代谢、碳代谢、光合作用	[25]
	愈伤组织、根、叶片和圆锥花序	抗体亲和富集、LC-MS/MS	890	1 536	蛋白质翻译、叶绿体发育、光合作用、开花和花粉肥力、根分生组织活力	[26]
	种子胚（吸胀24 h后）	抗体亲和富集、LC-MS/MS	389	699	翻译、刺激响应、葡萄糖分解代谢、糖酵解	[27]
	发育中的水稻花药（减数分裂时期）	抗体亲和富集、LC-MS/MS	676	1 354	染色质沉默、蛋白质折叠、脂肪酸生物合成过程、胁迫响应	[28]
	未授粉雌蕊、授粉三/7 d后的种子	抗体亲和富集、LC-MS/MS	972	1 817	糖酵解/糖异生、TCA循环、氨基酸合成、淀粉和蔗糖代谢	[29]
	盛花期15 d的水稻种子	抗体亲和富集、LC-MS/MS	692	1 003	TCA循环、糖酵解/糖异生、戊糖磷酸途径、淀粉合成和代谢、储藏蛋白	[30]
	叶片（氧化处理）	抗体亲和富集、LC-MS/MS	1 024	1 669	蛋白质翻译和折叠、光合作用、糖酵解	[31]
	幼苗叶片（冷胁迫）	抗体亲和富集、LC-MS/MS	866	1 353	光反应、卡尔文循环	[32]
小麦	幼苗叶片	抗体亲和富集、LC-MS/MS	277	416	光反应、卡尔文循环	[33]
小麦	发育中的种子（干旱胁迫）	抗体亲和富集、LC-MS/MS	442	716	碳代谢、淀粉生物合成、蛋白质运输和降解、胁迫响应、转录	[34]
二穗短柄草	幼苗叶片	抗体亲和富集、LC-MS/MS	353	636	碳代谢、光合作用、碳固定	[35]
大豆	发育中的种子	抗体亲和富集、LC-MS/MS	245	400	RNA合成和加工、乙酰、信号传导、蛋白质折叠	[36]
草莓	叶片	抗体亲和富集、LC-MS/MS	684	1 392	糖代谢、碳代谢、光合作用	[37]
云杉	部分干燥处理14 d的体细胞胚	抗体亲和富集、LC-MS/MS	556	1 079	碳代谢、脂肪酸代谢、胁迫响应、核糖体、蛋白酶体、剪接体	[38]
豌豆	线粒体	LC-MS/MS	358	664	初级代谢、次级代谢、胁迫反应、核酸代谢、蛋白质合成、蛋白质折叠	[39]
茶树	叶片（氮饥饿和恢复供氮）	抗体亲和富集、LC-MS/MS	1 286	2 229	光合作用、糖酵解、氨基酸代谢、次级代谢（黄酮合成）	[40]
硅藻	细胞（缺氮、缺磷和缺铁胁迫）	抗体亲和富集、LC-MS/MS	1 220	2 324	脂肪酸合成	[41]

植物	组织或器官（处理条件）	实验方法	蛋白质数	位点数	主要生物过程或代谢途径	参考文献
拟南芥	悬浮细胞、地上部、线粒体、叶绿体	抗体亲和富集、LC-MS/MS	74	91	光合作用	[19]
	叶片、长角果、花和种子、根	1/2-DE、LC-MS/MS	57	64	光合作用	[20]
	线粒体	抗体亲和富集、LC-MS/MS	120	243	TCA循环、呼吸链、光呼吸、氨基酸和蛋白质代谢、氧化还原调节	[5]
	叶片（赖氨酸去乙酰化酶抑制剂）	二甲基标记、抗体亲和富集、LC-MS/MS	1 022	2 152	光合作用	[21]
	21 d幼苗的地上部,乙烯处理	梯度离心、二甲基标记、抗体亲和富集、LC-MS/MS	2 638	7 456	组蛋白超家族、核糖体、热休克、胁迫/刺激、能量代谢	[22]
	不同组织（白天结束和晚上结束时）	抗体亲和富集、LC-MS/MS	909	1 365	捕光和光合作用、翻译、代谢、细胞运输	[23]
水稻	悬浮细胞	抗体亲和富集、LC-MS/MS	44	60	胁迫响应、DNA代谢、氮代谢、生物合成	[24]
	幼苗	抗体亲和富集、LC-MS/MS	716	1 337	乙醛酸和二羧酸代谢、碳代谢、光合作用	[25]
	愈伤组织、根、叶片和圆锥花序	抗体亲和富集、LC-MS/MS	890	1 536	蛋白质翻译、叶绿体发育、光合作用、开花和花粉肥力、根分生组织活力	[26]
	种子胚（吸胀24 h后）	抗体亲和富集、LC-MS/MS	389	699	翻译、刺激响应、葡萄糖分解代谢、糖酵解	[27]
	发育中的水稻花药（减数分裂时期）	抗体亲和富集、LC-MS/MS	676	1 354	染色质沉默、蛋白质折叠、脂肪酸生物合成过程、胁迫响应	[28]
	未授粉雌蕊、授粉三/7 d后的种子	抗体亲和富集、LC-MS/MS	972	1 817	糖酵解/糖异生、TCA循环、氨基酸合成、淀粉和蔗糖代谢	[29]
	盛花期15 d的水稻种子	抗体亲和富集、LC-MS/MS	692	1 003	TCA循环、糖酵解/糖异生、戊糖磷酸途径、淀粉合成和代谢、储藏蛋白	[30]
	叶片（氧化处理）	抗体亲和富集、LC-MS/MS	1 024	1 669	蛋白质翻译和折叠、光合作用、糖酵解	[31]
	幼苗叶片（冷胁迫）	抗体亲和富集、LC-MS/MS	866	1 353	光反应、卡尔文循环	[32]
小麦	幼苗叶片	抗体亲和富集、LC-MS/MS	277	416	光反应、卡尔文循环	[33]
小麦	发育中的种子（干旱胁迫）	抗体亲和富集、LC-MS/MS	442	716	碳代谢、淀粉生物合成、蛋白质运输和降解、胁迫响应、转录	[34]
二穗短柄草	幼苗叶片	抗体亲和富集、LC-MS/MS	353	636	碳代谢、光合作用、碳固定	[35]
大豆	发育中的种子	抗体亲和富集、LC-MS/MS	245	400	RNA合成和加工、乙酰、信号传导、蛋白质折叠	[36]
草莓	叶片	抗体亲和富集、LC-MS/MS	684	1 392	糖代谢、碳代谢、光合作用	[37]
云杉	部分干燥处理14 d的体细胞胚	抗体亲和富集、LC-MS/MS	556	1 079	碳代谢、脂肪酸代谢、胁迫响应、核糖体、蛋白酶体、剪接体	[38]
豌豆	线粒体	LC-MS/MS	358	664	初级代谢、次级代谢、胁迫反应、核酸代谢、蛋白质合成、蛋白质折叠	[39]
茶树	叶片（氮饥饿和恢复供氮）	抗体亲和富集、LC-MS/MS	1 286	2 229	光合作用、糖酵解、氨基酸代谢、次级代谢（黄酮合成）	[40]
硅藻	细胞（缺氮、缺磷和缺铁胁迫）	抗体亲和富集、LC-MS/MS	1 220	2 324	脂肪酸合成	[41]

植物	组织或器官	蛋白质数 /位点数	蛋白质比例/%		保守的乙酰化位点序列	参考文献
植物	组织或器官	蛋白质数 /位点数	1个位点	≥4个位点	保守的乙酰化位点序列	参考文献
拟南芥	地上部	2 638/7 456	56.6	12.6	KacR,KacR,KacK,KacK,K*Kac	[22]
水稻	种子	692/1 003	72	4	KacR,KacK,KacY,KacF,KacH,K*Kac	[30]
水稻	叶片	1 024/1 669		-	KacR,KacK,KacS,KacT,KacH,KacN	[31]
小麦	叶片	277/416	72	4	KacY,KacH,KacF,LKac,FKac	[33]
二穗短柄草	叶片	353/636	63	10	KacY,KacK,KacH,KacF,KacI*K	[35]
草莓	叶片	684/1 392	-	-	LKac,FKac,KacH,KacY,KacF	[37]
茶树	叶片	1 286/2 229	62	8	EKacK,KacK,KacR,KacHK,KacN,KacS,KacT,KacD	[40]
云杉	体细胞胚	556/1 079	57	13	KacY,KacF,FKac,YKac,KacH,KKac,VKac	[38]

植物	组织或器官	蛋白质数 /位点数	蛋白质比例/%		保守的乙酰化位点序列	参考文献
植物	组织或器官	蛋白质数 /位点数	1个位点	≥4个位点	保守的乙酰化位点序列	参考文献
拟南芥	地上部	2 638/7 456	56.6	12.6	KacR,KacR,KacK,KacK,K*Kac	[22]
水稻	种子	692/1 003	72	4	KacR,KacK,KacY,KacF,KacH,K*Kac	[30]
水稻	叶片	1 024/1 669		-	KacR,KacK,KacS,KacT,KacH,KacN	[31]
小麦	叶片	277/416	72	4	KacY,KacH,KacF,LKac,FKac	[33]
二穗短柄草	叶片	353/636	63	10	KacY,KacK,KacH,KacF,KacI*K	[35]
草莓	叶片	684/1 392	-	-	LKac,FKac,KacH,KacY,KacF	[37]
茶树	叶片	1 286/2 229	62	8	EKacK,KacK,KacR,KacHK,KacN,KacS,KacT,KacD	[40]
云杉	体细胞胚	556/1 079	57	13	KacY,KacF,FKac,YKac,KacH,KKac,VKac	[38]