重楼中一株产纤维素酶内生真菌的分离及鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2021-0266

生物技术通报 ›› 2022, Vol. 38 ›› Issue (2): 95-104.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0266

重楼中一株产纤维素酶内生真菌的分离及鉴定

张功友¹^,²^,³^,⁵(), 王一涵¹^,³, 郭敏¹^,³, 张婷婷¹^,³^,⁴, 王兵¹^,³^,⁴, 刘红美¹^,³^,⁴()

1.贵州医科大学医药生物技术工程研究中心,贵阳 550025
2.贵州医科大学药用植物功效与利用国家重点实验室,贵阳 550025
3.贵州医科大学生物与工程学院,贵阳 550025
4.贵州省高校免疫细胞与抗体工程研究中心,贵阳 550025
5.贵州医科大学基础医学院,贵阳 550025

收稿日期:2021-03-08 出版日期:2022-02-26 发布日期:2022-03-09
作者简介:张功友,男,硕士,研究方向：植物真菌学;E-mail： zgy1943541699@163.com
基金资助:
贵阳市联合基金([2017]5-33);贵州省科技厅科技支撑计划([2017]2833);贵州医科大学2019年研究生创新项目(YJSCXJH[2019]006号);学术新苗培育与创新探索项目(贵州科技平台人才[2018]5779-53号);省部共建药用植物功效与利用国家重点实验室自主课题资助项目(FAMP2021Z1-1)

Isolation and Identification of a Cellulase-producing Endophytic Fungus in Paris polyphylla var. yunnanensis

ZHANG Gong-you¹^,²^,³^,⁵(), WANG Yi-han¹^,³, GUO Min¹^,³, ZHANG Ting-ting¹^,³^,⁴, WANG Bing¹^,³^,⁴, LIU Hong-mei¹^,³^,⁴()

1. Engineering Research Center of Medical Biotechnology,Guizhou Medical University,Guiyang 550025
2. State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medcial University,Guiyang 550025
3. School of Biology and Engnieering,Guizhou Medical University,Guiyang 550025
4. Immune Cell and Antibody Engineering Research Center of Guizhou Province,Guiyang 550025
5. School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025

Received:2021-03-08 Published:2022-02-26 Online:2022-03-09

摘要/Abstract

摘要：

从重楼根茎中分离、鉴定具有产纤维素酶活性的内生真菌。采用表面消毒法从重楼块茎中分离内生真菌;用纤维素酶活性CMC平板检测分离菌株的产纤维素酶活性;对高产菌株进行形态学观察和分子生物学测序鉴定;探究影响纤维素酶活力的因素;利用平板法检测该株菌产其他胞外水解酶的活性。从3个来源的重楼中分离出41株内生真菌,通过平板检测发现AS-5、AS-7、AS-9和AS-18菌株能产生纤维素酶,其中AS-9菌株活性最强;通过形态学观察和ITS、LSU序列分析将AS-9菌株鉴定为Setophoma terrestris;该菌在pH值为7.0和温度为28℃时表现出最大纤维素酶活性,紫外线照射对产纤维素酶活性无明显作用;检测发现AS-9菌株同时具有产酪蛋白酶、脂肪酶、天冬酰胺酶、谷氨酰胺酶和脲酶活性。首次在重楼中发现内生真菌Setophoma terrestris,且具有较好的产纤维素酶能力,值得深入研究。

关键词: 纤维素酶, 重楼, 分离鉴定, Setophoma terrestris, 胞外水解酶

Abstract:

This paper aims to isolate and identify an endophytic fungus with cellulase activity in Paris polyphylla var. yunnanensis. Surface disinfection method was applied to isolate the endophytic fungi from P. polyphylla var. yunnanensis rhizome. CMC plate assay was used to detect the cellulase activity of the isolated strains. Morphological features and sequence characters based techniques were applied to identify the strains producing high-yield cellulase. The factors affecting the cellulase activity were also explored. Finally,plate activity screen method was used to test the activity of other extracellular hydrolases production. A total of 41 fungal endophytes were isolated from 3 origins of P. polyphylla var. yunnanensis. Among the 41 isolates,strains named as AS-5,AS-7,AS-9 and AS-18 were found to be able to produce cellulase,and strain AS-9 showed the strongest cellulase production activity. AS-9 was identified as Setophoma terrestris based on the morphological observations and ITS,LSU sequences analysis. AS-9 presented maximal cellulase activity at pH7.0 and 28℃. UV exposure did not have a significant effect on cellulase production of AS9. Meanwhile,S. terrestris AS-9 also demonstrated the activities of producing caseinase,lipase,asparaginase,glutaminase and urease production. To the best of our knowledge,S. terrestris AS-9 is first detected as an endophytic fungus in P. polyphylla var. yunnanensis and shows a better cellulase production capacity. It is worth for further study.

Key words: cellulase, Paris polyphylla var. yunnanensis, isolation and identification, Setophoma terrestris, extracellular hydrolytic enzyme

张功友, 王一涵, 郭敏, 张婷婷, 王兵, 刘红美. 重楼中一株产纤维素酶内生真菌的分离及鉴定[J]. 生物技术通报, 2022, 38(2): 95-104.

ZHANG Gong-you, WANG Yi-han, GUO Min, ZHANG Ting-ting, WANG Bing, LIU Hong-mei. Isolation and Identification of a Cellulase-producing Endophytic Fungus in Paris polyphylla var. yunnanensis[J]. Biotechnology Bulletin, 2022, 38(2): 95-104.

图/表 10

表1 重楼内生真菌的分离

Table 1 Isolation of fungal endophytes from P. polyphylla var. yunnanensis

位置Location	菌株编号Strain No.	来源Source	数量Number
105°58'13.22″E 26°02'22.85″N	AS-1,AS-2,AS-3,AS-4,AS-5,AS-7,AS-8,AS-9,AS-12,AS-13,AS-14,AS-15,AS-16,AS-17,AS-18,AS-19,AS-20	块茎Tuber	17
107°01'10.49″E 28°14'28.36″N	ZY-1,ZY-2,ZY-5,ZY-7,ZY-16	块茎Tuber	5
105°05'37.79″E 24°29'27.58″N	GX-E,GX-F,GX-G,GX-H,GX-I,GX-J,GX-K,GX-L,GX-M,GX-N,GX-O,GX-P,GX-Q,GX-R,GX-S,GX-T,GX-U,GX-V,GX-W	块茎Tuber	19

图1 产纤维素酶菌株的筛选

Fig. 1 Screening of cellulase-producing strains

表2 产纤维素酶内生真菌的透明圈大小

Table 2 Size of transparent circle produced by cellulase-producing endophytic fungus

菌株编号 Strain No.	透明圈直径Transparent circle diameter D/cm	菌落直径 Colony diameter d/cm	D/d
Penicillium	1.8	1.2	1.64
AS-5	1.5	0.7	2.14
AS-7	0.8	0.55	1.45
AS-9	1.7	0.75	2.27
AS-18	1.5	1.0	1.5

图2 AS-9菌株形态 A：菌落正面;B：菌落背面;C-F：光学显微镜观察菌丝形态;G：分生孢子;H,I：扫描电镜观察菌丝形态

Fig. 2 Morphology of AS-9 strain A：The colony surface. B：The colony dorsal surface. C-F：Fungal hyphae were observed using the optical microscope. G：Conidia. H,I：Fungal hyphae were observed using scanning electron microscopy

图3 ITS、LSU PCR扩增结果 M：DL2000 DNA marker. 1,2：ITS. 3,4：LSU

Fig. 3 Results of ITS and LSU PCR amplification

图4 基于ITS和LSU基因序列的系统进化树 A：基于ITS序列的系统进化树;B：基于LSU序列的系统进化树

Fig. 4 Phylogenetic trees based on the ITS and LSU gene sequences A：Phylogenetic tree based on the ITS sequence. B：Phylogenetic tree based on the LSU sequence

图5 不同pH、温度和紫外线对纤维素酶活性的影响 A：pH;B：温度;C：紫外照射

Fig. 5 Effects of different pH,temperature and UV on cellulase activity A：pH. B：Temperature. C：UV irradiation

表3 AS-9菌株不同培养条件产纤维素酶的透明圈大小

Table 3 Size of transparent circle produced by AS-9 strain under different culture conditions

培养条件Culture condition	透明圈直径 Transparent circle diameter D/cm	菌落直径 Colony diameter d/cm	D/d
pH4.0	1.5	0.95	1.58
pH5.0	1.5	1.05	1.43
pH6.0	1.4	0.85	1.65
pH7.0	1.9	0.75	2.53
pH8.0	1.3	1.05	1.24
25℃	1.7	0.75	2.27
28℃	2.0	0.8	2.5
32℃	1.3	0.55	2.36
28℃（无紫外）	1.7	0.65	2.61
28℃（紫外）	1.25	0.45	2.78

图6 纤维素酶活性的标准曲线

Fig. 6 Standard curve of cellulase activity

图7 胞外水解酶活性检测 A：酪蛋白酶;B：脂肪酶;C：L-天冬酰胺酶;D：谷氨酰胺酶;E：脲酶;F：明胶酶;G：植酸酶;H：果胶酶;I：淀粉酶;J：木质素酶

Fig. 7 Detection of extracellular hydrolytic enzyme activity A：Caseinase. B：Lipase. C：L-asparaginase. D：Glutaminase. E：Urease. F：Gelatinase. G：Phytase. H：Pectinase. I：Amylase. J：Ligninase

参考文献 48

[1]	郭晓华, 王仕宝. 中药材重楼研究进展[J]. 陕西农业科学, 2019, 65(5):94-98.
	Guo XH, Wang SB. Research progress of Chinese herbal medicine Polyphylla[J]. Shaanxi Journal of Agricultural Sciences, 65(5):94-98.
[2]	Ling LZ, Zhang SD, Zhao F, et al. Transcriptome-wide identification and prediction of miRNAs and their targets in paris Polyphylla var. Yunnanensis by high-throughput sequencing analysis[J]. Int J Mol Sci, 2017, 18(1):219. doi: 10.3390/ijms18010219 URL
[3]	代娇, 宋秋玲, 田甜. 关于滇重楼内生真菌抗菌活性的筛选和菌株鉴定研究[J]. 神州, 2019(1):227.
	Dai J, Song QL, Tian T. Study on the screening and strain identification of endophytic fungi from Paris polyphylla Smith var. yunnanensis[J]. Sheng Zhou, 2019(1):227.
[4]	张晨阳, 梁宗锁, 李先恩, 等. 七叶一枝花不同器官的成分含量变化规律[J]. 浙江理工大学学报:自然科学版, 2020, 43(6):831-839.
	Zhang CY, Liang ZS, Li XE, et al. Change law of component content of different organs in Paris Polyphylla[J]. J Zhejiang Sci Tech Univ:Nat Sci Ed, 2020, 43(6):831-839.
[5]	杨松岩. 纤维素酶在中药提取中的应用[J]. 世界中医药, 2015, 10(S1):474-475.
	Yang SY. Application of cellulase in extraction of traditional chinese medicine[J]. World Journal of Traditional Chinese Medicine, 2015, 10(S1):474-475.
[6]	Costa OYA, de Hollander M, Pijl A, et al. Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer[J]. Microbiome, 2020, 8(1):76. doi: 10.1186/s40168-020-00836-7 URL
[7]	Li X, Han C, Li W, et al. Insights into the cellulose degradation mechanism of the thermophilic fungus Chaetomium thermophilum based on integrated functional omics[J]. Biotechnol Biofuels, 2020, 13:143. doi: 10.1186/s13068-020-01783-z URL
[8]	杨吉霞, 蔡俊鹏, 祝玲. 纤维素酶在中药成分提取中的应用[J]. 中药材, 2005, 28(1):64-67.
	Yang JX, Cai JP, Zhu L. Application of ccellulase in extraction of traditional Chinese medicine[J]. J Chin Med Mater, 2005, 28(1):64-67.
[9]	Li F, Dong JY, Lv X, et al. Recombinant expression and characterization of two glycoside hydrolases from extreme alklinphilic bacterium Cellulomonas bogoriensis 69B4^T[J]. AMB Express, 2020, 10(1):1-10. doi: 10.1186/s13568-019-0926-y URL
[10]	王欣宇. 特异性产纤维素酶东北红豆杉内生真菌的筛选及初步应用[D]. 哈尔滨:东北林业大学, 2020.
	Wang XY. Screening of endophytic fungi of Taxus cuspidata s. et z. for specific production of cellulase and its preliminary application[D]. Harbin:Northeast Forestry University, 2020.
[11]	Ma LL, Zhao YC, Meng LM, et al. Isolation of thermostable lignocellulosic bacteria from chicken manure compost and a M42 family endocellulase cloning from Geobacillus thermodenitrificans Y7[J]. Front Microbiol, 2020, 11:281. doi: 10.3389/fmicb.2020.00281 URL
[12]	Mentges M, Bormann J. Real-time imaging of hydrogen peroxide dynamics in vegetative and pathogenic hyphae of Fusarium graminearum[J]. Sci Rep, 2015, 5:14980. doi: 10.1038/srep14980 URL
[13]	林英, 秦萍, 杜志强, 等. 产纤维素酶绿色木霉F-UV₂₆₄产酶条件优化[J]. 安徽农业科学, 2006, 34(11):2312-2314.
	Lin Y, Qin P, Du ZQ, et al. Study on optimization producing cellulase conditions of Trichoderma[J]. J Anhui Agric Sci, 2006, 34(11):2312-2314.
[14]	Li SJ, Zhang X, Wang XH, et al. Novel natural compounds from endophytic fungi with anticancer activity[J]. Eur J Med Chem, 2018, 156:316-343. doi: 10.1016/j.ejmech.2018.07.015 URL
[15]	Teimoori-Boghsani Y, Ganjeali A, Cernava T, et al. Endophytic fungi of native Salvia abrotanoides plants reveal high taxonomic diversity and unique profiles of secondary metabolites[J]. Front Microbiol, 2019, 10:3013. doi: 10.3389/fmicb.2019.03013 pmid: 32010087
[16]	Zhao J, Shan T, Mou Y, et al. Plant-derived bioactive compounds produced by endophytic fungi[J]. Mini Rev Med Chem, 2011, 11(2):159-168. pmid: 21222580
[17]	Liu JJ, Liu G. Analysis of secondary metabolites from plant endophytic fungi[J]. Methods Mol Biol Clifton N J, 2018, 1848:25-38.
[18]	Tyagi B, Tewari S, Dubey A. Biochemical characterization of fungus isolated during In vitro propagation of Bambusa balcooa[J]. Pharmacogn Mag, 2018, 13(suppl 4):S775-S779.
[19]	韩慧玲. 植物内生真菌来源纤溶酶的筛选及其酶学特性的探究[D]. 长春:吉林大学, 2019.
	Han HL. Screening of fibrinolytic enzyme from plant endophytic fungi and study on its enzymatic characteristics[D]. Changchun:Jilin University, 2019.
[20]	Wang Q, Shen SK, Zhang AL, et al. Isolation and diversity analyses of endophytic fungi from Paris Polyphylla var. Yunnanensis[J]. China J Chin Mater Med, 2013, 38(22):3838-3844.
[21]	Chung PC, Wu HY, Wang YW, et al. Diversity and pathogenicity of Colletotrichum species causing strawberry anthracnose in Taiwan and description of a new species, Colletotrichum miaoliense sp. nov[J]. Sci Rep, 2020, 10:14664. doi: 10.1038/s41598-020-70878-2 URL
[22]	Lenz AR, Galán-Vásquez E, Balbinot E, et al. Gene regulatory networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 inferred by a computational biology approach[J]. Front Microbiol, 2020, 11:588263. doi: 10.3389/fmicb.2020.588263 URL
[23]	Gupta A, Jana AK. Effects of wheat straw solid contents in fermentation media on utilization of soluble/insoluble nutrient, fungal growth and laccase production[J]. 3 Biotech, 2018, 8(1):35. doi: 10.1007/s13205-017-1054-5 URL
[24]	杨瑞先, 张拦, 彭彪彪, 等. 芍药内生真菌的鉴定及产生活性次生代谢产物的评估[J]. 微生物学报, 2017, 57(10):1567-1582.
	Yang RX, Zhang L, Peng BB, et al. Fungal endophytes in Paeonia lactiflora and their secondary metabolites[J]. Acta Microbiol Sin, 2017, 57(10):1567-1582.
[25]	Trakunyingcharoen T, Lombard L, Groenewald JZ, et al. Mycoparasitic species of Sphaerellopsis, and allied lichenicolous and other genera[J]. IMA Fungus, 2014, 5(2):391-414. doi: 10.5598/imafungus.2014.05.02.05 pmid: 25734030
[26]	Farha AK, Hatha AM. Bioprospecting potential and secondary metabolite profile of a novel sediment-derived fungus Penicillium sp. ArCSPf from continental slope of Eastern Arabian Sea[J]. Mycology, 2019, 10(2):109-117. doi: 10.1080/21501203.2019.1572034 URL
[27]	Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193(1):265-275. doi: 10.1016/S0021-9258(19)52451-6 URL
[28]	Guo QQ, Du GC, Qi HT, et al. A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode(Bursaphelenchus xylophilus)[J]. Pestic Biochem Physiol, 2017, 135:64-68. doi: 10.1016/j.pestbp.2016.06.003 URL
[29]	Farha AK, Tr T, Purushothaman A, et al. Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea[J]. J Genet Eng Biotechnol, 2018, 16(2):253-258. doi: 10.1016/j.jgeb.2018.06.002 URL
[30]	El-Naggar NE, El-Shweihy NM. Bioprocess development for L-asparaginase production by Streptomyces rochei, purification and in-vitro efficacy against various human carcinoma cell lines[J]. Sci Rep, 2020, 10(1):7942. doi: 10.1038/s41598-020-64052-x pmid: 32409719
[31]	满意, 魏铭, 王慧凯. 中药重楼活性成分抗肿瘤的作用机制[J]. 药学研究, 2016, 35(6):355-356.
	Man Y, Wei M, Wang HK. The anti-tumor effects of Paris Polyphylla’s Action Mechanism[J]. J Pharm Res, 2016, 35(6):355-356. doi: 10.1006/phrs.1997.0132 URL
[32]	Duan Z, Shen R, Liu B, et al. Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete[J]. AMB Express, 2018, 8(1):119. doi: 10.1186/s13568-018-0648-6 URL
[33]	杨喆茗, 张益铭, 许伟民, 等. 纤维素酶辅助法提取柚子皮中多糖的工艺研究[J]. 人参研究, 2013, 25(2):31-34.
	Yang ZM, Zhang YM, Xu WM, et al. Study on extraction process of polysaccharide from grapefruit peel by cellulase-assistant extraction[J]. Ginseng Res, 2013, 25(2):31-34.
[34]	许云峰. 银杏内生菌纤维素酶的分离、纯化及其在银杏总黄酮提取中的应用研究[D]. 苏州:苏州大学, 2009.
	Xu YF. The isolation and purification of endophytic fungal cellulase and its application in total flavonoid extraction from Ginkgo biloba L[D]. Suzhou:Soochow University, 2009.
[35]	邢来君, 李明春. 普通真菌学[M]. 北京: 高等教育出版社, 1999.
	Xing LJ, Li MC. General Mycology[M]. Beijing: Higher Education Press, 1999.
[36]	López-López M, Léon-Félix J, Allende-Molar R, et al. First report of Setophoma terrestris causing corky and pink root of tomato in Sinaloa, Mexico[J]. Plant Dis, 2020, 104(5):1553.
[37]	Albarracín Orio AG, Petras D, Tobares RA, et al. Fungal-bacterial interaction selects for quorum sensing mutants with increased production of natural antifungal compounds[J]. Commun Biol, 2020, 3(1):670. doi: 10.1038/s42003-020-01342-0 pmid: 33184402
[38]	李云龙. 微生物农药[J]. 中学生物教学, 2002(Z2):23.
	Li YL. Microbial pesticides[J]. Biol Teaghing Middle Sch, 2002(Z2):23.
[39]	de Gruyter J, Woudenberg JH, Aveskamp MM, et al. Systematic reappraisal of species in Phoma section Paraphoma, Pyrenochaeta and Pleurophoma[J]. Mycologia, 2010, 102(5):1066-1081. doi: 10.3852/09-240 URL
[40]	Arora D, Chashoo G, Singamaneni V, et al. Bacillus amyloliquefaciens induces production of a novel blennolide K in coculture of Setophoma terrestris[J]. J Appl Microbiol, 2018, 124(3):730-739. doi: 10.1111/jam.13683 pmid: 29288594
[41]	Islam F, Roy N. Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses[J]. BMC Res Notes, 2018, 11(1):1-6. doi: 10.1186/s13104-017-3088-5 URL
[42]	Prasanna HN, Ramanjaneyulu G, Rajasekhar Reddy B. Optimization of cellulase production by Penicillium sp[J]. 3 Biotech, 2016, 6(2):162. doi: 10.1007/s13205-016-0483-x pmid: 28330234
[43]	王禄山, 曲音波. 纤维素酶高产菌种选育及酶活测定[J]. 生物产业技术, 2008(2):56-61.
	Wang LS, Qu YB. Breeding of high-producing cellulase strains and determination of enzyme activity[J]. Biotechnol Bus, 2008(2):56-61.
[44]	安宝聚. 高产纤维素酶黑曲霉ANSTJ01菌株的分离鉴定与生物学性状探究[D]. 泰安:山东农业大学, 2017.
	An BJ. Identification and exploration of biological characteristics of Aspergillus niger strain ANSTJ01 with high cellulase production[D]. Tai’an:Shandong Agricultural University, 2017.
[45]	杨建宇, 刘冠军, 刘白云, 等. 中华中医药道地药材系列汇讲(8)道地药材滇重楼的研究近况[J]. 现代医学与健康研究电子杂志, 2020, 4(8):117-120.
	Yang JY, Liu GJ, Liu BY, et al. A series of lectures on authentic medicinal materials of Chinese Traditional Chinese Medicine(8)Recent research on authentic medicinal material Paris polyphylla Smith var. yunnanensis[J]. Mod Med Heal Res Electron J, 2020, 4(8):117-120.
[46]	张晓云, 任可, 李维蛟. 通过ITS序列分析鉴定云南重楼内生真菌[J]. 江西农业学报, 2020, 32(3):42-47.
	Zhang XY, Ren K, Li WJ. Identification of endophytic fungi by its sequence analysis in Paris Polyphylla var. Yunnanensis[J]. Acta Agric Jiangxi, 2020, 32(3):42-47.
[47]	Plyasova AA, Pokrovskaya MV, Lisitsyna OM, et al. Penetration into cancer cells via clathrin-dependent mechanism allows L-asparaginase from Rhodospirillum rubrum to inhibit telomerase[J]. Pharmaceuticals, 2020, 13(10):286. doi: 10.3390/ph13100286 URL
[48]	Awad MF, El-Shenawy FS, El-Gendy MMAA, et al. Purification, characterization, and anticancer and antioxidant activities of L-glutaminase from Aspergillus versicolor Faesay4[J]. Int Microbiol, 2021, 24(2):169-181. doi: 10.1007/s10123-020-00156-8 URL

重楼中一株产纤维素酶内生真菌的分离及鉴定

Isolation and Identification of a Cellulase-producing Endophytic Fungus in Paris polyphylla var. yunnanensis

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