杆状病毒-昆虫细胞表达系统在复合体重组表达应用中的研究进展

摘要/Abstract

摘要： 真核细胞大部分生命活动是通过复合体催化的。因此，系统了解这些复合体的生物学功能，将在健康和疾病方面具有重要意义。由于内源性复合体存在低丰度和异质性特点，因而直接提取复合体存在一定的困难。杆状病毒-昆虫细胞表达系统可成功表达复合体，为研究复合体的结构和功能提供新的手段。综述近年来利用杆状病毒-昆虫细胞表达系统进行蛋白质复合体结构和功能研究的进展。

关键词: 杆状病毒-昆虫细胞表达系统, 复合体, 晶体结构, 功能

Abstract: Most essential functions of eukaryotic cells are catalyzed by complex built of many subunits. To well understand their biological function in the process of health and disease, it is imperative to study these complexes. The low abundance and heterogeneity of many essential complexes have limited their extraction from native source material. The baculovirus expression vector system（BEVS）, specifically tailored for multiprotein complex production, has been proven itself to be uniquely suited for overcoming this impeding bottleneck. Here we highlight recent major achievements in multiprotein complex structure research which were catalyzed by this versatile recombinant complex expression tool.

万婧, 相兴伟, 江玲丽, 周向阳. 杆状病毒-昆虫细胞表达系统在复合体重组表达应用中的研究进展[J]. 生物技术通报, 2014, 0(2): 7-14.

Wan Jing, Xiang Xingwei, Jiang Lingli, Zhou Xiangyang,. Advances on the Baculovirus Expression Vector System for Protein Complex Production[J]. Biotechnology Bulletin, 2014, 0(2): 7-14.

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