波茨坦短芽孢杆菌降解苯酚特性及动力学研究

摘要/Abstract

摘要： 从活性污泥中分离筛选出一株高效苯酚降解菌，经形态特征、生理生化试验及16S rDNA鉴定，该菌株为波茨坦短芽孢杆菌。该菌能以苯酚为唯一碳源和能源，最佳降解条件为:温度30℃，初始pH7.0，摇床转速为160 r/min。苯酚降解试验表明，该菌可在72 h内将初始浓度为1 600 mg/L苯酚完全降解。随着苯酚浓度的增加，底物抑制作用增强。应用Haldane方程对菌株的生长过程进行动力学模拟，拟合曲线与试验测定值相关性良好，各参数分别为μmax(最大比增长率)0.334 h ^-1，Ks(半饱和常数)14.07 mg/L，Ki(抑制常数)196.89 mg/L，且该菌株苯酚降解动力学与其生长动力学表现出相似的趋势。代谢机制研究表明，苯酚可诱导该菌合成邻苯二酚1，2-加氧酶降解苯酚。

关键词: 波茨坦短芽孢杆菌, 苯酚, 生物降解, 动力学

Abstract: An effective phenol-degrading strain was isolated from activated sludge, and was identified as Brevibacillus borstelensis based on morphological, biochemical, physiological experiments and 16S rDNA gene sequence analysis. The strain could utilize phenol as sole carbon and energy source and the optimum degradation condition were temperature of 30℃, initial pH7.0 and shaking speed of 160 r/min. The biodegradation experiment indicated that the strain could completely degrade 1 600 mg/L phenol in 72 h. Also, the results showed that the higher the phenol concentration was, the stronger the substrate inhibition imposed. The process of its growth was also investigated using Haldane kinetic model. The parameters were:μmax(maximum specific growth rate)=0.334 h ^-1, Ks(half-satruration constant)=14.07 mg/L, Ki(inhibition constant)=196.89 mg/L. The phenol degradation kinetics of the strain followed a similar trend to that of its growth. Metabolic pathway research showed that the strain could be induced to synthesize extracellular catechol-1, 2-dioxygenase.

Key words: Brevibacillus borstelensis, Phenol, Biodegradation, Kinetics

葛启隆, 王国英,岳秀萍. 波茨坦短芽孢杆菌降解苯酚特性及动力学研究[J]. 生物技术通报, 2014, 0(3): 117-122.

Ge Qilong, Wang Guoying, Yue Xiuping. Phenol Degradation by Brevibacillus borstelensis and Kinetic Analysis[J]. Biotechnology Bulletin, 2014, 0(3): 117-122.

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