黑曲霉（AS0006）产纤维素酶的纯化研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (6): 187-191.

黑曲霉（AS0006）产纤维素酶的纯化研究

张欢¹, 曹焱鑫¹, 蒋林², 王晓明¹, 刘齐¹, 董晓莹¹, 寇巍¹

（1.辽宁省能源研究所，营口 115003；2.营口市疾病预防控制中心，营口 115003）

收稿日期:2013-11-03 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:张欢，女，助理研究员，研究方向：木质纤维素燃料乙醇技术；E-mail：zhanghuan032987@163.com
基金资助:
国家“863”课题（2013AA050701）

Study on Purification of Cellulase from Aspergillus niger AS0006

Zhang Huan¹,Cao Yanxin¹,Jiang Lin²,Wang Xiaoming¹,Liu Qi¹,Dong Xiaoying¹,Kou Wei¹

（1. Liaoning Institute of Energy Resources，Yingkou 115003；2. Yingkou Center for Disease Control and Prevention，Yingkou 115003）

Received:2013-11-03 Published:2014-06-25 Online:2014-06-25

摘要/Abstract

摘要： 利用实验室中黑曲霉菌株AS0006进行粗酶发酵，对产出的纤维素酶液进行不同饱和度（NH₄）₂SO₄的分级沉淀，经过Sephadex G-25、Sephadex G-100分离纯化后，得到两种内切酶（EG）组分、一种外切酶（CBH）组分。通过酶活性及电泳检测发现，两种EG酶组分的相对分子质量分别为29.63 kD、37.49 kD，CBH酶组分相对分子质量为56.51 kD。相较原始粗酶液，EG回收率可达18.07%、纯化倍数为3.46，CBH回收率可达12.96%、纯化倍数为4.14，β-葡萄糖苷酶（CB）回收率可达22.37%、纯化倍数为3.58。

关键词: 黑曲霉, 纤维素酶, 分离纯化

Abstract: Two endoglucanases（EG）components and a cellobiohydrolase（CBH）from Aspergillus niger AS0006 were separated and purified by（NH₄）₂SO₄ fractional precipitation, Sephadex G-25 and Sephadex G-100 chromatography. According to cellulase activity and electrophoresis detection found that the molecular weight of two EG components were 29.63 kD and 37.49 kD, respectively, the molecular weight of CBH component was 56.51 kD. Compared to the crude enzyme, the recovery rate of EG components could reach 18.07%, the purification fold was 3.46, the recovery rate of CBH component could reach 12.96%, the purification fold was 4.14, the recovery rate of β-glucosidase（CB）could reach 22.37% and the purification fold was 3.58.

Key words: Aspergillus niger, Cellulase, Purification

张欢, 曹焱鑫, 蒋林, 王晓明, 刘齐, 董晓莹, 寇巍. 黑曲霉（AS0006）产纤维素酶的纯化研究[J]. 生物技术通报, 2014, 0(6): 187-191.

Zhang Huan,Cao Yanxin,Jiang Lin,Wang Xiaoming,Liu Qi,Dong Xiaoying,Kou Wei. Study on Purification of Cellulase from Aspergillus niger AS0006[J]. Biotechnology Bulletin, 2014, 0(6): 187-191.

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