植物蛋白磷酸酶2C在非生物胁迫信号通路中的调控作用

摘要/Abstract

摘要：

植物在非生物胁迫下会产生一系列的形态、生理生化和分子水平上的适应性变化，尤其是非生物胁迫会引起植物体内的蛋白磷酸酶2C（PP2C）基因表达的改变，从而诱导植物合成相关的蛋白以适应胁迫。植物中有不同类型的PP2C亚群，各种PP2C亚群能够通过不同的信号途径参与胁迫应答，因此在植物响应非生物胁迫的过程中发挥重要作用。综述了植物PP2C在非生物胁迫信号通路中的作用机制。

关键词: 植物蛋白磷酸酶2C, 非生物胁迫, 信号通路, 胁迫应答, 调控

Abstract:

Abiotic stress could cause a series of changes to plants in morphological, physiological, biochemical and molecular level. Especially the abiotic stresses would lead protein phosphorylase PP2C related gene expression change. At the same time, the proteins related biosynthesis induced by the abiotic stresses would improve plants resistance. However, the different PP2C through the different signaling pathways involved in abiotic stress. This article introduced the regulative mechanisms of PP2C mediated the abiotic stress signal pathways.

Key words: Plant protein phosphatasesⅡC, Abiotic stress, Signaling pathway, Stress response, Regulation

杜驰, 张富春. 植物蛋白磷酸酶2C在非生物胁迫信号通路中的调控作用[J]. 生物技术通报, 2014, 0(8): 16-22.

Du Chi, Zhang Fuchun. Protein Phosphatases Ⅱ C in Plants are Involved in Abiotic Stress Tolerance of Several Signaling Pathways[J]. Biotechnology Bulletin, 2014, 0(8): 16-22.

参考文献

［1］张春宝, 赵丽梅, 赵洪锟, 等. 植物蛋白激酶研究进展［J］. 生物技术通报, 2011（10）：17-23.
［2］Banks JA. Selaginella and 400 million years of separation［J］. Annual Review of Plant Biology, 2009, 60：223-238.
［3］Goff SA, Ricke D, Lan TH, et al. A draft sequence of the rice genome（Oryza sativa L. ssp. japonica）［J］. Science, 2002, 296（5565）：92-100.
［4］Komatsu K, Suzuki N, Kuwamura M, et al. Group A PP2Cs evolved in land plants as key regulators of intrinsic desiccation tolerance［J］. Nature Communications, 2013, 4：2219.
［5］Merchant SS, Prochnik SE, Vallon O, et al. The Chlamydomonas genome reveals the evolution of key animal and plant functions［J］. Science, 2007, 318（5848）：245-250.
［6］Rensing SA, Lang D, Zimmer AD, et al. The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants［J］. Science, 2008, 319（5859）：64-69.
［7］Galletti R, Ferrari S, De Lorenzo G. Arabidopsis MPK3 and MPK6 play different roles in basal and oligogalacturonide-or flagellin-induced resistance against Botrytis cinerea［J］. Plant Physiol, 2011, 157（2）：804-814.
［8］胡帅, 王芳展, 刘振宁, 等. PYR/PYL/RCAR 蛋白介导植物ABA 的信号转导［J］. 遗传, 2012, 34（5）：560-572 .
［9］Raghavendra AS, Gonugunta VK, Christmann A, et al. ABA perception and signalling［J］. Trends Plant Sci, 2010, 15（7）：395-401.
［10］Umezawa T, Nakashima K, Miyakawa T, et al. Molecular basis of the core regulatory network in ABA responses：sensing, signaling and transport［J］. Plant and Cell Physiology, 2010, 51（11）：1821-1839.
［11］Daszkowska-Golec A, Wojnar W, Rosikiewicz M, et al. Arabidopsis suppressor mutant of abh1 shows a new face of the already known players：ABH1（CBP80）and ABI4-in response to ABA and abiotic stresses during seed germination［J］. Plant Molecular Biology, 2013, 81（1-2）：189-209.
［12］Leung J, Merlot S, Giraudat J. The Arabidopsis ABSCISIC ACID-INSENSITIVE2（ABI2）and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduc-tion［J］. The Plant Cell Online, 1997, 9（5）：759-771.
［13］Rodriguez PL, Benning G, Grill E. ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis［J］. FEBS Letters, 1998, 421（3）：185-190.
［14］Merlot S, Gosti F, Guerrier D, et al. The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway［J］. The Plant Journal, 2001, 25（3）：295-303.
［15］Fujita Y, Fujita M, Shinozaki K, et al. ABA-mediated transcriptional regulation in response to osmotic stress in plants［J］. Journal of Plant Research, 2011, 124（4）：509-525.
［16］Umezawa T, Sugiyama N, Mizoguchi M, et al. Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis［J］. Proc Natl Acad Sci USA, 2009, 106（41）：17588-17593.
［17］Yoshida T, Nishimura N, Kitahata N, et al. ABA-hypersensitive germination3 encodes a protein phosphatase 2C（AtPP2CA）that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs［J］. Plant Physiology, 2006, 140（1）：115-126.
［18］Koornneef M, Reuling G, Karssen CM. The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana［J］. Physiologia Plantarum, 1984, 61（3）：377-383.
［19］Rodriguez PL, Leube MP, Grill E. Molecular cloning in Arabidopsis thaliana of a new protein phosphatase 2C（PP2C）with homology to ABI1 and ABI2［J］. Plant Molecular Biology, 1998, 38（5）：879-883.
［20］Fujita Y, Nakashima K, Yoshida T, et al. Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis［J］. Plant and Cell Physiology, 2009, 50（12）：2123-2132.
［21］Nishimura N, Yoshida T, Kitahata N, et al. ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed［J］. The Plant Journal, 2007, 50（6）：935-949.
［22］Szostkiewicz I, Richter K, Kepka M, et al. Closely related receptor complexes differ in their ABA selectivity and sensitivity［J］. The Plant Journal, 2010, 61（1）：25-35.
［23］Brandt B, Brodsky DE, Xue S, et al. Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action［J］. Proc Natil Acad Sci USA, 2012, 109（26）：10593-10598.
［24］Saez A, Rodrigues A, Santiago J, et al. HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis［J］. Plant Cell, 2008, 20（11）：2972-2988.
［25］Ohta M, Guo Y, Halfter U, et al. A novel domain in the protein kin-ase SOS2 mediates interaction with the protein phosphatase 2C AB-I2［J］. Proc Natl Acad Sci USA, 2003, 100（20）：11771-11776.
［26］Ma Y, Szostkiewicz I, Korte A, et al. Regulators of PP2C phosphatase activity function as abscisic acid sensors［J］. Science, 2009, 324（5930）：1064-1068.
［27］陈金焕, 夏新莉, 尹伟伦. 植物 2C 类蛋白磷酸酶及其在逆境信号转导中的作用［J］. 北京林业大学学报, 2010（5）：168-171.
［28］Melcher K, Ng LM, Zhou XE, et al. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors［J］. Nature, 2009, 462（7273）：602-608.
［29］Bhaskara GB, Nguyen TT, Verslues PE. Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs［J］. Plant Physiology, 2012, 160（1）：379-395.
［30］Sun HL, Wang XJ, Ding WH, et al. Identification of an important site for function of the type 2C protein phosphatase ABI2 in abscisic acid signalling in Arabidopsis［J］. Journal of Experimental Botany, 2011, 62（15）：5713-5725.
［31］Antoni R, Gonzalez-Guzman M, Rodriguez L, et al. PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root［J］. Plant Physiol, 2013, 161（2）：931-941.
［32］Kiegerl S, Cardinale F, Siligan C, et al. SIMKK, a mitogen-activated protein kinase（MAPK）kinase, is a specific activator of the salt stress-induced MAPK, SIMK［J］. The Plant Cell Online, 2000, 12（11）：2247-2258.
［33］Schweighofer A, Kazanaviciute V, Scheikl E, et al. The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis［J］. The Plant Cell Online, 2007, 19（7）：2213-2224.
［34］吴涛, 宗晓娟, 谷令坤, 等. 植物中的 MAPK 及其在信号传导中的作用［J］. 生物技术通报, 2006（5）：1-7.
［35］Brock AK, Willmann R, Kolb D, et al. The Arabidopsis mitogen-activated protein kinase phosphatase PP2C5 affects seed germination, stomatal aperture, and abscisic acid-inducible gene expression［J］. Plant Physiology, 2010, 153（3）：1098-1111.
［36］Umbrasaite J, Schweighofer A, Kazanaviciute V, et al. MAPK phosphatase AP2C3 induces ectopic proliferation of epidermal cells leading to stomata development in Arabidopsis［J］. PloS One, 2010, 5（12）：e15357.
［37］Xing Y, Jia W, Zhang J. AtMKK1 and AtMPK6 are involved in abscisic acid and sugar signaling in Arabidopsis seed germination［J］. Plant Molecular Biology, 2009, 70（6）：725-736.
［38］Tang D, Christiansen KM, Innes RW. Regulation of plant disease resistance, stress responses, cell death, and ethylene signaling in Arabidopsis by the EDR1 protein kinase［J］. Plant Physiology, 2005, 138（2）：1018-1026.
［39］Schwarz US, Bischofs IB. Physical determinants of cell organization in soft media［J］. Medical Engineering & Physics, 2005, 27（9）：763-772.
［40］Wang P, Song CP. Guard-cell signalling for hydrogen peroxide and abscisic acid［J］. New Phytologist, 2008, 178（4）：703-718.
［41］Andreasson E, Ellis B. Convergence and specificity in the Arabidopsis MAPK nexus［J］. Trends in Plant Science, 2010, 15（2）：106-113.
［42］El-Maarouf-Bouteau H, Bailly C. Oxidative signaling in seed germination and dormancy［J］. Plant Signaling & Behavior, 2008, 3（3）：175.
［43］Jammes F, Song C, Shin D, et al. MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling［J］. Proc Natl Acad Sci USA, 2009, 106（48）：20520-20525.
［44］Wang XJ, Zhu SY, Lu YF, et al. Two coupled components of the mitogen-activated protein kinase cascade MdMPK1 and MdMKK1 from apple function in ABA signal transduction［J］. Plant and Cell Physiology, 2010, 51（5）：754-766.
［45］Benhamed M, Bertrand C, Servet C, et al. Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression［J］. The Plant Cell Online, 2006, 18（11）：2893-2903.
［46］Earley KW, Shook MS, Brower-Toland B, et al. In vitro specificities of Arabidopsis co-activator histone acetyltransferases：implications for histone hyperacetylation in gene activation［J］. The Plant Journal, 2007, 52（4）：615-626.
［47］Widjaja I, Lassowskat I, Bethke G, et al. A protein phosphatase 2C, responsive to the bacterial effector AvrRpm1 but not to the AvrB effector, regulates defense responses in Arabidopsis［J］. The Plant Journal, 2010, 61（2）：249-258.
［48］Bertrand C, Bergounioux C, Domenichini S, et al. Arabidopsis histone acetyltransferase AtGCN5 regulates the floral meristem activity through the WUSCHEL/AGAMOUS pathway［J］. Journal of Biological Chemistry, 2003, 278（30）：28246-28251.
［49］Long JA, Ohno C, Smith ZR, et al. TOPLESS regulates apical embryonic fate in Arabidopsis［J］. Science, 2006, 312（5779）：1520-1523.
［50］Puthiyaveetil S, Ibrahim IM, Allen JF. Oxidation-reduction signalling components in regulatory pathways of state transitions and photosystem stoichiometry adjustment in chloroplasts［J］. Plant Cell Environ, 2012, 35（2）：347-359.

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