牦牛PAFR基因生物信息学分析及其在妊娠前后子宫中的表达

生物技术通报 ›› 2014, Vol. 0 ›› Issue (8): 82-88.

牦牛PAFR基因生物信息学分析及其在妊娠前后子宫中的表达

魏博, 潘阳阳, 禹尧, 田枫, 余四九

甘肃农业大学动物医学院, 兰州 730070

修回日期:2014-01-09 出版日期:2014-08-15 发布日期:2014-08-01
作者简介:作者简介: 魏博，男，硕士研究生，研究方向：动物生殖生理；E-mail：jl_wb_cloud@163.com

Cloning and Sequence Analysis of Yak PAFR Gene and Expression in Uterus Before and After Pregnancy

Wei Bo, Pan Yangyang, Yu Yao, Tian Feng, Yu Sijiu

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070

Revised:2014-01-09 Published:2014-08-15 Online:2014-08-01
Supported by:
国家自然科学基金项目(32172616)

摘要/Abstract

摘要：

以牦牛血小板活化因子受体（Platelet-activating factor receptor，PAFR）基因为研究对象，采取西宁家养牦牛子宫为材料，采用RT-PCR技术克隆牦牛PAFR基因，对其进行生物信息学分析，并采用QRT-PCR方法定量检测PAFR在牦牛子宫不同时期的表达。结果表明，牦牛PAFR基因编码区长1 029 bp，编码342个氨基酸；氨基酸序列与黄牛同源性最高为99.7%，与非洲爪蟾蜍同源性最低为59.1%，表明PAFR氨基酸在进化过程中具有保守性；其编码蛋白的氨基酸序列有5个跨膜区域，推测其可能为跨膜蛋白；具有亲水性；未发现信号肽片段；含有G蛋白偶联受体家族结构域；QRT-PCR检测PAFR在牦牛子宫中表达，妊娠期最高，卵泡期最低，黄体期居中，揭示其与胚胎附植，妊娠维持有关。

关键词: 牦牛, PAFR, 基因克隆, 生物信息学分析, 基因表达

Abstract:

Platelet-activating factor receptor（PAFR）gene were cloned from uterus of domestic yak by reverse transcription polymerase chain reaction（RT-PCR）. The results shows that domestic yak PAFR gene was 1 029 bp in length, encoded predicted mature protein of 342 amino acids, which encoded a hydrophilic protein. Compared with the reference sequence of PCR primer designing homology of the domestic yak PAFR coding sequence was 99.7% at the amino acid level, and also showed high homology of with other species. The PAFR has 5 transmembrane domains, features G protein-coupled receptor domains, no signal peptide. The result of the quantitative Real-time Polymerase chain reaction（QRT-PCR）shows that PAFR was highest expression in gestation and lowest in follicular phase in uterus of domestic yak. That suggested PAFR play an important role in implantation and gestation.

Key words: Yak, PAFR, Gene cloning, Bioinformatic analysis, Gene expression

魏博, 潘阳阳, 禹尧, 田枫, 余四九. 牦牛PAFR基因生物信息学分析及其在妊娠前后子宫中的表达[J]. 生物技术通报, 2014, 0(8): 82-88.

Wei Bo, Pan Yangyang, Yu Yao, Tian Feng, Yu Sijiu. Cloning and Sequence Analysis of Yak PAFR Gene and Expression in Uterus Before and After Pregnancy[J]. Biotechnology Bulletin, 2014, 0(8): 82-88.

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