拟南芥PKS2与CAM4蛋白互作研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.013

生物技术通报 ›› 2015, Vol. 31 ›› Issue (2): 91-96.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.013

拟南芥PKS2与CAM4蛋白互作研究

乔新荣¹, 段鸿斌¹, 刘柱明¹, 赵付安²

1.信阳农林学院，信阳 464000;
2.河南省农业科学院经济作物研究所，郑州 450002

收稿日期:2014-07-28 出版日期:2015-02-05 发布日期:2015-02-06
作者简介:乔新荣，女，博士，讲师，研究方向：植物分子育种及植物生理；E-mail：xirong806@163.com

Research of PKS2 Interaction with CAM4 in Arabidopsis thailana

Qiao Xinrong^1,*, Duan Hongbin^1,*, Liu Zhuming^1,*, Zhao Fuan²

1. Xinyang College of Agriculture and Forestry，Xinyang 464000;
2. CashCropResearchInstitute，HenanAcademyofAgricultural Sciences，Zhengzhou 450002

Received:2014-07-28 Published:2015-02-05 Online:2015-02-06

摘要/Abstract

摘要： 植物蓝光受体向光素（phototropin，PHOT）介导许多生理反应，现已从拟南芥中分离了其下游的一些信号转导组分。前期研究表明，拟南芥光敏色素底物 PKS家族成员PKS1与部分Ca²⁺结合蛋白钙调素（calmodulin，CAM）成员互作，参与PHOT2介导的强蓝光诱导下胚轴向光反应。旨在探讨PKS2和CAM4之间的互作关系，首先用RT-PCR技术得到PKS2和CAM4 的cDNA全长序列。通过酵母双杂交和双分子荧光互补技术，从体外与体内证实PKS2和CAM4能相互作用。此结果进一步丰富了PKS家族与CAM之间的联系，为深入解析PHOT功能研究奠定基础。

关键词: 拟南芥, 向光素, 光敏色素底物, 钙调素

Abstract: Blue-light receptors phototropin （PHOT）mediate a wide set of physiological and developmental responses. As some PHOT downstream signal transduction components have been identified in Arabidopsis by genetic analyses, we have found that PKS1 of Phytochrome kinase substrate PKS family interacts with members of calmodulin （CAM）, which are Ca²⁺ binding protein. In order to address the issue of how PKS2 interacts with CAM4, firstly cDNA full length sequences of PKS2 and CAM4 were obtained by RT-PCR technique. Then, it was confirmed that PKS2 could be interact with CAM4 by tests of yeast two-hybrid system and bimolecular fluorescence complementation. These date could contribute to enrich relation between PKS and CAM, which would provide base for further uncovering PHOT functions.

Key words: Arabidopsis thaliana, phototropin, phytochrome kinase substrate, calmodulin

乔新荣, 段鸿斌, 刘柱明, 赵付安. 拟南芥PKS2与CAM4蛋白互作研究[J]. 生物技术通报, 2015, 31(2): 91-96.

Qiao Xinrong, Duan Hongbin, Liu Zhuming, Zhao Fuan. Research of PKS2 Interaction with CAM4 in Arabidopsis thailana[J]. Biotechnology Bulletin, 2015, 31(2): 91-96.

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拟南芥PKS2与CAM4蛋白互作研究

Research of PKS2 Interaction with CAM4 in Arabidopsis thailana

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