新疆野生及栽培荒漠肉苁蓉提取物免疫活性研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.022

生物技术通报 ›› 2016, Vol. 32 ›› Issue (1): 131-137.doi: 10.13560/j.cnki.biotech.bull.1985.2016.01.022

新疆野生及栽培荒漠肉苁蓉提取物免疫活性研究

杨秀梅¹, 杨雨¹, 王丹阳¹, 吴道澄², 张爱莲¹

1.新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室, 乌鲁木齐 830046;
2.西安交通大学生命科学与技术学院, 西安 710000

收稿日期:2015-08-18 出版日期:2016-01-09 发布日期:2016-01-22
作者简介:杨秀梅, 女, 硕士研究生, 研究方向：新型疫苗佐剂的研究;E-mail：444375019@qq.com
基金资助:
新疆维吾尔自治区科技援疆项目(201591150)

The Study of Immune Activities of Extracts from Wild and Cultivated Cistanche deserticola in Xinjiang

YANG Xiu-mei¹, YANG Yu¹, WANG Dan-yang¹, WU Dao-cheng², ZHANG Ai-lian¹

1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urmqi 830046;
2. College of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710000

Received:2015-08-18 Published:2016-01-09 Online:2016-01-22

摘要/Abstract

摘要： 旨在比较新疆野生与栽培荒漠肉苁蓉提取物主要活性成分差异, 并检测其诱导小鼠体内树突状细胞(Dendritic cells, DC)成熟能力, 评价其免疫活性。采用超声法制备提取物, 紫外法测定多糖及苯乙醇苷类化合物含量;脚掌免疫小鼠, 流式细胞术检测其对小鼠体内CD11c⁺DCs表面CD86(Cell differentiation antigens 86, CD86)和MHCII(Major histocompatibility complex II, MHCII)表达情况。结果表明, 野生及栽培荒漠肉苁蓉水提物中多糖含量分别为59.58%和76.82%, 醇提物中苯乙醇苷类化合物含量分别为17.12%和8.47%;小鼠免疫实验结果表明, 野生和栽培荒漠肉苁蓉提取物可显著增强小鼠体内CD11c⁺DCs表面CD86和MHCII上调表达(P<0.01), 且效果与阳性对照组LPS相当, 相同剂量野生与栽培醇提物除对CD86的作用差异显著外(P<0.05), 其它提取物之间对CD86和MHCII的作用均无显著差异(P>0.05)。新疆野生和栽培荒漠肉苁蓉主要成分之间存在一定差异, 且在适宜浓度下均可显著促进小鼠体内DCs成熟, 且差异不大。

关键词: 荒漠肉苁蓉, 多糖, 苯乙醇苷类化合物, 树突状细胞

Abstract: This work is to compare the differences of major active components in the extracts between wild and cultivated Cistanche deserticola in Xinjiang, detect their capacities to induce the maturation of dendritic cells(DCs)in mice, and evaluate their immune activities. The extracts were prepared by ultrasonic extraction, and the contents of polysaccharides and phenylethanoid glycosides were measured by ultraviolet-visible spectrophotometer method. The immune to BALB/c mice with extracts was through foot pad, and the expressions of CD86(cell differentiation antigens 86)and MHCII(major histocompatibility complex II)on the surface of CD11c⁺DCs inside mouse were detected by flow cytometry. The polysaccharides contents in the aqueous extracts from wild and cultivated C. deserticola were 59.58% and 76.82% respectively, and the contents of phenylethanoid glycosides in the alcohol extracts were 17.12% and 8.48% respectively. The immune tests of mice showed that the extracts from both wild and cultivated C. deserticola enhanced the expression level of CD86and MHCII on the surface of CD11c⁺DCs inside mice(P<0.01);and the effect was similar to that of the positive control group(LPS). The effect of the same dosage of wild and cultivated ethanol extracts on the CD86 was significantly different(P<0.05). The effect of the other extracts on MHCII and CD86 was not significantly different(P>0.05). In conclusion, there were differences in the main components between Xinjiang wild and cultivated C. deserticola, and in appropriate concentration they both could significantly promoted DCs maturation in mice, and the difference was not significant.

Key words: Cistanche deserticola, polysaccharides, phenylethanoid glycosides, dendritic cell

杨秀梅, 杨雨, 王丹阳, 吴道澄, 张爱莲. 新疆野生及栽培荒漠肉苁蓉提取物免疫活性研究[J]. 生物技术通报, 2016, 32(1): 131-137.

YANG Xiu-mei, YANG Yu, WANG Dan-yang, WU Dao-cheng, ZHANG Ai-lian. The Study of Immune Activities of Extracts from Wild and Cultivated Cistanche deserticola in Xinjiang[J]. Biotechnology Bulletin, 2016, 32(1): 131-137.

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新疆野生及栽培荒漠肉苁蓉提取物免疫活性研究

The Study of Immune Activities of Extracts from Wild and Cultivated Cistanche deserticola in Xinjiang

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