生物技术通报 ›› 2016, Vol. 32 ›› Issue (4): 24-33.doi: 10.13560/j.cnki.biotech.bull.1985.2016.04.003

• 综述与专论 • 上一篇    下一篇

真菌漆酶的性质、生产及应用研究进展

刘家扬12,焦国宝2,有小娟1,廖祥儒3,孙利鹏2   

  1. 1. 黄淮学院生物工程系,驻马店 463000;
    2. 河南仰韶生化工程有限公司,三门峡 472400;
    3. 江南大学生物工程学院,无锡 214122
  • 收稿日期:2015-06-14 出版日期:2016-04-25 发布日期:2016-04-26
  • 作者简介:刘家扬,男,博士,副教授,研究方向:酶的生产及应用;Email:liujiayang5@sohu.com
  • 基金资助:
    黄淮学院引进人才科研项目(1000.12.10.1342)

A Review on Properties,Production,and Application of Fungal Laccases

LIU Jia-yang12, JIAO Guo-bao2 ,YOU Xiao-juan1 ,LIAO Xiang-ru3, SUN Li-peng2   

  1. 1. Department of Bioengineering,Huanghuai University,Zhumadian 463000;
    2. Henan Yangshao Biochemical Engineering Co. Ltd.,Sanmenxia 472400;
    3. School of Biotechnology,Jiangnan University,Wuxi 214122
  • Received:2015-06-14 Published:2016-04-25 Online:2016-04-26

摘要: 作为一种含铜的多酚氧化酶,真菌漆酶比细菌漆酶、植物漆酶等具有更好的热稳定性、金属离子耐受性及更高的底物催化氧化性,在工农业及环境领域的应用中得到了较高的关注。目前普遍认为,限制漆酶广泛应用的因素在于漆酶的生产规模、成本与性质。真菌漆酶的生产模式包括固态发酵和液体发酵,工业生产基本以液体发酵为主。除了在染料脱色、染织废水处理、纸浆漂白等过程中的应用,最新的研究不断拓展了漆酶新的用途,对近年来真菌漆酶的发酵生产、酶学性质及应用研究中的最新结果进行了概述。

关键词: 白腐真菌, 漆酶, 发酵, 酶学性质, 应用

Abstract: As one of copper-containing oxidoreductases,fungal laccase shows better thermal stability,higher metal tolerance and catalytic oxidative to substrates than bacterial or plant ones,and thereby draws great attentions on applications in industry,agriculture and environmental protection. It is widely considered that the factors limiting the extensive application of laccase are production scale,process cost as well as its properties. Laccase can be produced via solid or liquid state fermentation,and the latter is usually adopted for industrial purposes. Recent progress has expanded the novel applications of laccase in addition to its conventional uses such as dye decolorization,treating wastewater of dyeing and paper pulp bleaching. This paper reviews the published literatures in recent years while focusing on the production,properties,and application of fungal laccase.

Key words: white-rot fungi, laccase, fermentation, enzymatic properties, application