植物AP2/ERF转录因子家族的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-1142

摘要/Abstract

摘要： 作为植物最大转录因子家族之一,AP2/ERF转录因子广泛存在于植物中,并因其在基因育种等方面具有重要作用而倍受关注。AP2/ERF转录因子家族成员至少含有一段60个左右氨基酸构成的AP2保守结构域,根据结构域的数量和识别序列的不同可以将其分为AP2、ERF、DREB、RAV和Soloist 5个亚家族,且在拟南芥、水稻、玉米和番茄等植物中,AP2/ERF转录因子及其各个亚家族成员的数量各不相同。研究发现,AP2/ERF转录因子可通过响应乙烯、细胞分裂素和生长素的调节从而直接或间接参与种子发育过程、花和果实等器官的形态建成等植物发育的多个进程;除了初生代谢,AP2/ERF转录因子还在植物次生代谢尤其是在调控药用植物主要药用活性成分（如青蒿素、紫杉醇和木质素）合成方面效果显著。同时,有报道称拟南芥AP2/ERF基因具有正向调节抗灰霉病的功能,一些AP2/ERF基因也被报道在植物应对高盐、干旱、缺氧、低温等非生物胁迫方面具重要功能;另外,AP2/ERF类转录因子还参与了乙烯等介导的非生物信号传导。介绍AP2/ERF转录因子的结构分类特征、在不同植物中的数量分布,并阐述其在植物发育、次生代谢、生物与非生物胁迫和信号传导等方面的研究进展,以期为培育出兼具高产、抗病的实用型转基因作物提供理论依据。

关键词: 转录因子, 植物发育, 次生代谢, 胁迫应答, 信号传导

Abstract: As one of the largest transcription factor family,AP2/ERF transcription factors are common in plants,and have attracted much attention because of their important role in gene breeding. The AP2/ERF transcription factor family contains at least one AP2 conservative domain with about 60 amino acids. According to the number of domains and the sequence of recognition,AP2/ERF transcription factor family can be classified into 5 subfamilies of AP2,ERF,DREB,RAV,and Soloist;and the number of AP2/ERF transcription factor and their subfamily members are variable in different plants,such as Arabidopsis,rice,maize,tomato,etc. Through responses to the regulation of ethylene,cytokinin and auxin,AP2/ERF transcription factors are reported to participate directly or indirectly in multiple processes of plant development including seed development,flower and fruit organ formation. Except for primary metabolism,AP2/ERF transcription factors affect significantly the regulation of plant secondary metabolism,particularly in improving the accumulation of active ingredients of medicinal plants（such as artemisinin,paclitaxel and lignin）. Meanwhile,the AP2/ERF gene of Arabidopsis are also reported to have a positive regulatory function of resistance to Botrytis cinerea,while some AP2/ERFgenes play a vital role in response to drought,high salt,low temperature,hypoxia and other abiotic stresses. In addition,the AP2/ERF transcription factors also participate in the non-biological signal transduction pathways mediated by ethylene. In this study,the structure and classification characteristics of AP2/ERFtranscription factors,their quantity distribution in different plants will be introduced,and the roles of AP2/ERF transcription factors in plant development,secondary metabolism,biotic or abiotic stress and signal transduction will be expounded. Overall,this review is aimed to provide theory basis for cultivating transgenic crops with high-yielding and stress tolerances in the future.

Key words: transcription factors, plant development, secondary metabolism, stress response, signal transduction

张麒, 陈静, 李俐, 赵明珠, 张美萍, 王义. 植物AP2/ERF转录因子家族的研究进展[J]. 生物技术通报, 2018, 34(8): 1-7.

ZHANG Qi, CHEN Jing, LI Li, ZHAO Ming-zhu, ZHANG Mei-ping, WANG Yi. Research Progress on Plant AP2/ERF Transcription Factor Family[J]. Biotechnology Bulletin, 2018, 34(8): 1-7.

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