高粱组织培养技术研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0235

摘要/Abstract

摘要： 高粱是全球第五大谷类作物,是食品、饲料和加工业的原料,也是一种潜在的能源作物,具有重要的应用价值。然而,通过基因工程技术对高粱进行遗传改良却十分困难,其主要原因是缺乏高效的组织培养体系。结合近年来的研究,从基因型、外植体、培养基、植物激素等影响高粱组织培养的主要因素进行综述,重点讨论了促进高粱再生的策略,并提出解决高粱组培再生困难的两大研究方向：一是深入研究植物再生的遗传及表观遗传机制;二是根据各基因型的代谢特点,进一步优化培养条件,旨为建立高效的高粱组织培养体系提供参考。

关键词: 植物组织培养, 基因型, 外植体, 培养基, 植物激素

Abstract: Sorghum（Sorghum bicolor L. Moench）is the fifth most widely planted cereal crop in the world. It is the raw material in food,fodder and processing industry. As well,it has been identified as a potential biofuel crop. Despite the important application value,the genetic improvement of sorghum via genetic engineering technology is very difficult,because there is no highly efficient sorghum tissue culture system. According to recent studies,we reviewed the main factors having great influences on sorghum tissue culture,such as genotypes,explants,media,plant hormones,etc. Importantly,we focused on the strategies which may promote the regeneration of sorghum. At the end,two directions for solving the difficulties in sorghum regeneration via tissue culture are given as：first,the genetic and epigenetical mechanisms of plant regeneration should be studied deeply;second,the culture conditions should be further optimized based on the metabolism characteristic of every genotype. These ideas will be references for the establishment of highly efficient sorghum tissue culture system.

Key words: plant tissue culture, genotype, explant, media, plant hormone

彭祥, 张德春. 高粱组织培养技术研究进展[J]. 生物技术通报, 2018, 34(10): 41-48.

PENG Xiang, ZHANG De-chun. Research Advances on Tissue Culture Technology of Sorghum[J]. Biotechnology Bulletin, 2018, 34(10): 41-48.

参考文献

[1] 张丽敏, 刘智全, 陈冰嬬, 等. 我国能源甜高粱育种现状及应用前景[J]. 中国农业大学学报, 2012, 17(6):76-82.
[2] Dahlberg J, Berenji J, Sikora V, et al.Assessing sorghum[Sorghum bicolor(L)Moench]germplasm for new traits:food, fuels & unique uses[J]. Maydica, 2011, 56:85-92.
[3] Vermerris W.Survey of genomics approaches to improve bioenergy traits in maize, sorghum and sugarcane[J]. Journal of Integrative Plant Biology, 2011, 53(2):105-119.
[4] 刘公社, 周庆源, 宋松泉, 等. 能源植物甜高粱种质资源和分子生物学研究进展[J]. 植物学报, 2009, 44(3):253-261.
[5] Chandrashekar A, Satyanarayana KV.Disease and pest resistance in grains of sorghum and millets[J]. Journal of Cereal Science, 2006, 44(3):287-304.
[6] Nwanze KF, Seetharama N, Sharma HC, et al.Biotechnology inpest management:Improving resistance in sorghum to insect pests[J]. African Crop Science, 1995, 3(2):209-215.
[7] Chen M, Shelton A, Ye GY, et al.Insect-resistant genetically modified rice in China:From research to commercialization[J]. Annual Review of Entomology, 2011, 56:81-101.
[8] Zhang BH.Transgenic cotton:From biotransformation methods to agricultural application[J]. Methods in Molecular Biology, 2013, 958:3-15.
[9] Calla B, Blahut-Beatty L, Koziol L, et al.Genomic evaluation of oxalate-degrading transgenic soybean in response to sclerotinia sclerotiorum infection[J]. Molecular Plant Pathology, 2014, 15(6):563-575.
[10] Polumahanthi S, Manin S, Pola S, et al.Tissue culture, molecular and genetic approaches to sorghum crop improvement[J]. Indian Journal of Plant Sciences, 2015, 4(2):97-113.
[11] Liu GQ, Gilding EK, Godwin ID.A robust tissue culture system for sorghum[Sorghum bicolor(L.)Moench][J]. South African Journal of Botany, 2015, 98:157-160.
[12] Liu GQ, Godwin ID.Highly efficient sorghum transformation[J]. Plant Cell Report, 2012, 31(6):999-1007.
[13] Wu E, Lenderts B, Glassman K, et al.Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants[J]. In Vitro Cell Dev Biol Plant, 2014, 50(1):9-18.
[14] Do PT, Lee H, Mookkan M, et al.Rapid and efficient Agrobacterium-mediated transformati on of sorghum(Sorghum bicolor)employing standard binary vectors and bar gene as a selectable marker[J]. Plant Cell Report, 2016, 35(10):2065-2076.
[15] Hiei Y, Ishida Y, Komari. Progress of cereal transformation technology mediated by Agrobacterium tumefaciens[J]. Frontiers in Plant Science, 2014, 5:1-11.
[16] Li JQ, Wang LH, Zhan QW, et al.Development of a simple and efficient method for Agrobacterium-mediated transformation in sorghum[J]. International Journal of Agriculture & Biology, 2015, 18(1):134-138.
[17] Lowe K, Wu E, Wang N, et al.Morphogenic regulators Baby boom and Wuschel improve monocot transformation[J]. Plant Cell, 2016, 28(9):1998-2015.
[18] Cai T, Butler L.Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghums[J]. Plant Cell, Tissue and Organ Culture, 1990, 20(2):101-110 .
[19] Assem SK, Zamzam M, Hussein BA, et al.Evaluation of somatic embryogenesis and plant regeneration in tissue culture of ten sorghum(Sorghum bicolor L.)genotype[J]. African Journal of Biotechnology, 2014, 13(36):3672-3681.
[20] Gamhorg OL, Shyluk JP, Brar DS, et al.Morphogenesis and plant regeneration from callus of immature embryos of sorghum[J]. Plant Science Letters, 1977, 10(1):67-74.
[21] Dustan DI, Short KC, Dhaliwal H, et al.Further studies on plantlet production from cultured tissues of sorghum[J]. Protoplasma, 1979, 101(4):355-361.
[22] Ma HT, Gu MH, Liang GH.Plant regeneration from cultured immature embryos of Sorghum bicolor(L.)Moench[J]. Theorl Appl Genet, 1987, 73(3):389-394.
[23] 马鸿图, Liang GH.高粱幼胚培养及再生植株变异的研究[J]. 遗传学报, 1985, 12(5):350-357.
[24] 韩福光, 张颍. 高粱不同外植体愈伤组织诱导的研究[J]. 辽宁农业科学, 1993, (1):45-48.
[25] Brettell RIS, Wernicke W, Thomas E.Embryogenesis from cultured immature inflorescences of Sorghum bicolor[J]. Protoplasma, 1980, 104(1-2):141-148.
[26] Jogeswar G, Ranadheer D, Anjaiah V, et al.High frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor(L.)Moench from immature inflorescence explants[J]. In Vitro Cell Dev Biol Plant, 2007, 43(2):159-166.
[27] Murashige T, Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J]. Physiologia Plantarum, 1962, 15:473-497.
[28] Gupta S, Khanna VK, Singh R, et al.Strategies for overcoming genotypic limitations of in vitro regeneration and determination of genetic components of variability of plant regeneration traits in sorghum[J]. Plant Cell Tiss Organ Cult, 2006, 86(3):379-388.
[29] 徐丹, 陈立余, 徐子勤. 甜高粱离体再生体系的建立和组织结构变化的观察[J]. 植物生理学通讯, 2009, 45(8):771-774.
[30] 赵利铭. 甜高粱遗传背景对其再生能力的影响及再生体系的建立[D]. 合肥:中国科学技术大学, 2010.
[31] Pola S, Saradamani N, Ramana T.Mature embryos as a source material for efficient regeneration response in sorghum(Sorghum bicolor L. Moench)[J]. Sjemenarstvo, 2009, 26:3-4.
[32] Wernicke W, Potrykus I, Thomas E.Morphogenesis from cultured leaf tissue of Sorghum bicolor — The morphogenetic pathway[J]. ProtopIasma, 1982, 111(1):53-62.
[33] Pola S.Leaf discs as a source material for plant tissue culture studies of Sorghum bicolor(L.)Moench[J]. Not Sci Biol, 2011, 3(1):70-78.
[34] Pola SR, Mani NS.Somatic embryogenesis and plantlet regeneration in Sorghum bicolor(L.)Moench from leaf segments[J]. Journal of Cell and Molecular Biology, 2006, 5:99-107.
[35] Wen FS, Sorensen EL, Barnett F L, et al.Callus induction and plant regeneration from anther and inflorescence culture of Sorghum[J]. Euphytica, 1991, 52(3):177-181.
[36] Nirwan RS, Kothari SL.High frequency shoot organogenesis in Sorghum bicolor(L)Moench[J]. J. Plant Biochemistry & Biotechnology, 2004, 13(2):149-152.
[37] Kishore NS, Visarada K, Lakshmi YA, et al.In vitro culture methods in sorghum with shoot tip as the explant material[J]. Plant Cell Rep, 2006, 25(3):174-182.
[38] 刘宣雨, 刘树君, 宋松泉. 建立甜高粱高频、高效再生体系的研究[J]. 中国农业科学, 2010, 43(23):4963-4969.
[39] Baskaran P, Rajeswari BR, Jayabalan N.Development of an in vitro regeneration system in Sorghum[Sorghum bicolor(L.)Moench]using root transverse thin cell layers(tTCLs)[J]. Turk J Bot, 2006, 30:1-9.
[40] Sticklen MB, Oraby H.Shoot apical meristem:a sustainable explant for genetic transformation of cereal crops[J]. In Vitro Cell Dev Bio Plant, 2005, 41(3):187-200.
[41] Maekinnon C, Gunderson G, Nabors MW.Plant regeneration by somatic embryogenesis from callus cultures of sweet sorghum[J]. Plant Cell Reports, 1986, 5(5):349-351.
[42] Linsmaier EM, Skoog F.Organic growth factor requirements of tobacco tissue cultures[J]. Physiologia Plantarum, 1965, 18:100-127.
[43] Goh E J, Seong E S, Yoo J H, et al.Effect of plant growth regulators and media on regeneration of Sorghum bicolor(L.)Moench[J]. Korean J. Plant Res. , 2011, 24(2):168-173.
[44] Chu CC, Wang CC, Sun CS, et al.Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources[J]. Scientia Sinica, 1975, 18(5):659-668.
[45] Gamborg OL, Miller RA, Ojima K.Nutrient requments of suspension cultures of soybean root cells[J]. Expremental Cell Research, 1968, 50:151-158.
[46] Elkonin LA, Pakhomova NV.Influence of nitrogen and phosphorus on induction embryogenic callus of sorghum[J]. Plant Cell, Tissue and Organ Culture, 2000, 61(2):115-123.
[47] Kumar V, Campbell LM, Rathore KS.Rapid recovery and characterization of transformants following Agrobacterium-mediated T-DNA transfer to sorghum[J]. Plant Cell Tiss Organ Cult, 2011, 104(2):137-146.
[48] Purnhauser L.Stimulation of shoot and root regeneration in wheat(Triticum aestivum)callus cultures by copper[J]. Cereal Research Communication, 1991, 19(4):419-423.
[49] Purnhauser L, Gyulai G.Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures[J]. Plant Cell Tissue and Organ Culture, 1993, 35(2):131-139.
[50] Dahleen L.Improved plant regeneration from barley callus cultures by increased copper levels[J]. Plant Cell Tissue Organ Culture, 1995, 43(3):267-269.
[51] Nirwan RS, Kothar SL.High copper levels improve callus induction and plant regeneration in Sorghum bicolor(L.)Moench In Vitro Cell Dev Biol Plant, 2003, 39(2):161-164.
[52] Liu GQ, Gilding EK, Godwin ID.Additive effects of three auxins and copper on sorghum in vitro root induction[J]. In Vitro Cell Dev Biol Plant, 2013, 49(2):19-197.
[53] Lidon FC, Barreiro MG, Henriquest F.Interactions between biomass production and ethylene biosynthesis in copper treated rice[J]. Journal of Plant Nutrition, 1995, 18(6):1301-1314.
[54] Rao AM, Sree KP, Kishor PBK.Enhanced plant regeneration in grain and sweet sorghum by asparagine, proline and cefotaxime[J]. Plant Cell Reports, 1995, 15(1-2):72-75.
[55] Elkonin LA, Lopushanskaya RF, Pakhomova NV.Initiation and maintenance of friable, embryogenetic callus of sorghum(Sorghum bicolor(L.)Moench)by amino acids[J]. Maydica, 1995, 40(2):153-157.
[56] Hagio T.Adventitious shoot regeneration from immature embryos of sorghum[J]. Plant Cell, Tissue and Organ Culture, 2002, 68(1):65-72.
[57] Nguyen TV, Thu TT, Claeys M, et al.Agrobacterium mediated transformation of sorghum(Sorghum bicolor(L.)Moench)using an improved in vitro regeneration system[J]. Plant Cell Tiss and Organ Cult, 2007, 91(2):155-164.
[58] Lu L, Wu XG, Yin XY, et al.Development of marker-free transgenic sorghum[Sorghum bicolor(L.)Moench]using standard binary vectors with bar as a selectable marker[J]. Plant cell Tiss Organ Cult, 2009, 99(1):97-108.
[59] Che P, Anand A, Wu E, et al.Developing a flexible, high-efficiency Agrobacterium-mediated sorghum transformation system with broad application[J]. Plant Biotechnology Journal, 2018:1-8.
[60] Kumar V, Staden JV.New insights into plant somatic embryogenesis:an epigenetic view[J]. Acta Physiol Plant, 2017, 39:1-17.