生物技术通报 ›› 2021, Vol. 37 ›› Issue (10): 216-224.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0244
收稿日期:
2021-03-03
出版日期:
2021-10-26
发布日期:
2021-11-12
作者简介:
邓普荣,女,硕士研究生,研究方向:生物安全与生物多样性;E-mail: 基金资助:
Received:
2021-03-03
Published:
2021-10-26
Online:
2021-11-12
摘要:
转基因抗虫作物降低了昆虫的危害并减少了农药的使用,但是转Bt(Bacillus thuringiensis)基因抗虫作物的大面积连续种植使得靶标害虫对转Bt基因植物产生抗性进化,导致其长期抗虫效果受到挑战。为延缓靶标害虫对Bt蛋白的抗性进化,提高转基因作物的抗虫能力,RNA干扰(RNA interference,RNAi)技术与转Bt基因技术协同应用于农业害虫防治。本文综述了RNAi技术应用于农业害虫防控的进展,以及与转基因抗虫技术联合防治农业害虫的研究进展,分析其面临的挑战和技术难题,以期支撑RNAi与转基因技术发展成一种绿色环保和可持续发展的害虫综合防控技术。
邓普荣, 刘勇波. RNAi与转Bt基因技术协同抗虫研究进展[J]. 生物技术通报, 2021, 37(10): 216-224.
DENG Pu-rong, LIU Yong-bo. Review on the Synergistic Insect-resistant Application of RNAi and Bt-transgenic Technologies[J]. Biotechnology Bulletin, 2021, 37(10): 216-224.
物种 Species | 目标基因 Target gene | Bt蛋白基因 Bt gene | 联合作效率 Synergistic efficiency | 参考文献Reference |
---|---|---|---|---|
马铃薯甲虫 Leptinotarsa decemlineata | prohibitin-1 | Cry3Aa | 死亡 Mortality | [56] |
海灰翅夜蛾 Spodoptera littoralis | Sl102 | Cry1Ca | 免疫力下降 Down-regulation of immunocompetence | [57] |
亚洲玉米螟 Ostrinia furnacalis | chymotrypsin-like genes | Cry1Ab | 死亡率显著升高 Significantly increased mortalities | [60] |
甜菜夜蛾 Spodoptera exigua | CHS-B | Cry1Ac 和Cry1Ca | 死亡 Mortality | [61] |
棉铃虫 Helicoverpa armigera | JHAMT和JHBP | Cry1Ac | 死亡 Mortality | [6] |
棉铃虫 Helicoverpa armigera | v-ATPase-A | Cry1Ac | 体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac | [62] |
表1 RNAi与转Bt基因技术协同抗虫
Table 1 Synergistic application of RNAi and Bt-transgenic technologies in controlling pests
物种 Species | 目标基因 Target gene | Bt蛋白基因 Bt gene | 联合作效率 Synergistic efficiency | 参考文献Reference |
---|---|---|---|---|
马铃薯甲虫 Leptinotarsa decemlineata | prohibitin-1 | Cry3Aa | 死亡 Mortality | [56] |
海灰翅夜蛾 Spodoptera littoralis | Sl102 | Cry1Ca | 免疫力下降 Down-regulation of immunocompetence | [57] |
亚洲玉米螟 Ostrinia furnacalis | chymotrypsin-like genes | Cry1Ab | 死亡率显著升高 Significantly increased mortalities | [60] |
甜菜夜蛾 Spodoptera exigua | CHS-B | Cry1Ac 和Cry1Ca | 死亡 Mortality | [61] |
棉铃虫 Helicoverpa armigera | JHAMT和JHBP | Cry1Ac | 死亡 Mortality | [6] |
棉铃虫 Helicoverpa armigera | v-ATPase-A | Cry1Ac | 体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac | [62] |
干扰途径 RNAi approach | 植物 Plant | 昆虫 Insect | 目标基因 Target gene | 参考文献 Reference |
---|---|---|---|---|
宿主诱导的基因沉默 HIGS | 玉米和大豆 Zea mays and Glycine max | 绿盲蝽 Apolygus lucorum | V-ATPase-E | [39] |
本氏烟 Nicotiana benthamiana | 桃蚜 Myzus persicae | MpC002 and Rack-1 | [36] | |
拟南芥 Arabidopsis thaliana | 桃蚜 Myzus persicae | MySP | [44] | |
烟草 Nicotiana tabacum | 烟粉虱 Bemisia tabaci | acetylcholinesterase(AChE) ecdysone receptor(EcR) | [37] | |
小麦 Triticum aestivum | 麦长管蚜 Sitobion avenae | zinc finger(SaZFP) | [40] | |
马铃薯 Solanum tuberosum | 马铃薯甲虫 Leptinotarsa decemlineata | EcR | [38] | |
拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum | 棉铃虫 Helicoverpa armigera | CYP6AE14 | [41] | |
拟南芥 Arabidopsis thaliana | 棉铃虫 Helicoverpa armigera | mitochondrial complex I(NDUFV2) | [19] | |
饲喂法 Dietary feeding | 豌豆蚜 Acyrthosiphon pisum | ApAQP1 | [76] | |
显微注射法 Microinjection | 黄曲条跳甲 Phyllotreta striolata | odorant receptor(PsOr1) | [77] | |
德国小蠊 Blattella germanica | RXR | [22] | ||
微生物介导 Bacterial expression and oral delivery | 粘虫 Mythimna separata | MseChi | [15] | |
亚洲玉米螟 Ostrinia furnacalis | Glutathione-S-Transferase | [78] |
表2 RNAi技术应用于农业害虫防控
Table 2 RNAi technology applied in controlling agricultural pests
干扰途径 RNAi approach | 植物 Plant | 昆虫 Insect | 目标基因 Target gene | 参考文献 Reference |
---|---|---|---|---|
宿主诱导的基因沉默 HIGS | 玉米和大豆 Zea mays and Glycine max | 绿盲蝽 Apolygus lucorum | V-ATPase-E | [39] |
本氏烟 Nicotiana benthamiana | 桃蚜 Myzus persicae | MpC002 and Rack-1 | [36] | |
拟南芥 Arabidopsis thaliana | 桃蚜 Myzus persicae | MySP | [44] | |
烟草 Nicotiana tabacum | 烟粉虱 Bemisia tabaci | acetylcholinesterase(AChE) ecdysone receptor(EcR) | [37] | |
小麦 Triticum aestivum | 麦长管蚜 Sitobion avenae | zinc finger(SaZFP) | [40] | |
马铃薯 Solanum tuberosum | 马铃薯甲虫 Leptinotarsa decemlineata | EcR | [38] | |
拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum | 棉铃虫 Helicoverpa armigera | CYP6AE14 | [41] | |
拟南芥 Arabidopsis thaliana | 棉铃虫 Helicoverpa armigera | mitochondrial complex I(NDUFV2) | [19] | |
饲喂法 Dietary feeding | 豌豆蚜 Acyrthosiphon pisum | ApAQP1 | [76] | |
显微注射法 Microinjection | 黄曲条跳甲 Phyllotreta striolata | odorant receptor(PsOr1) | [77] | |
德国小蠊 Blattella germanica | RXR | [22] | ||
微生物介导 Bacterial expression and oral delivery | 粘虫 Mythimna separata | MseChi | [15] | |
亚洲玉米螟 Ostrinia furnacalis | Glutathione-S-Transferase | [78] |
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