生物技术通报 ›› 2022, Vol. 38 ›› Issue (3): 41-49.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0716

• 研究报告 • 上一篇    下一篇

茉莉花萜类合成酶基因的转录组鉴定及响应外源激素的表达研究

洪雅萍(), 陈雪津, 王鹏杰, 谷梦雅, 高婷, 叶乃兴()   

  1. 福建农林大学园艺学院/茶学福建省高校重点实验室,福州 350002
  • 收稿日期:2021-06-04 出版日期:2022-03-26 发布日期:2022-04-06
  • 作者简介:洪雅萍,女,硕士研究生,研究方向:茶树栽培和资源利用;E-mail: 1025953179@qq.com
  • 基金资助:
    福州市科技计划项目(2019-N-15);福州茉莉花茶科技与全球重要农业文化遗产联合研究中心专项;福建张天福茶叶发展基金会科技创新基金(FJZTF01)

Transcriptome Identification of Terpenoid Synthase Genes in Jasminum sambac and Their Expressions Responding to Exogenous Hormones

HONG Ya-ping(), CHEN Xue-jin, WANG Peng-jie, GU Meng-ya, GAO Ting, YE Nai-xing()   

  1. College of Horticulture,Fujian Agriculture and Forestry University,Key Laboratory of Tea Science at Universities in Fujian,Fuzhou 350002
  • Received:2021-06-04 Published:2022-03-26 Online:2022-04-06

摘要:

探究萜类合成酶(Terpenoid synthase,TPS)在茉莉花萜类化合物合成中的作用及外源激素处理下的表达模式,为深入研究茉莉花TPS基因家族的功能及在外源激素胁迫中的作用奠定理论依据。以茉莉花转录组数据为基础,利用生物信息学方法对茉莉花TPS家族进行鉴定及分析,采用实时荧光定量分析茉莉花TPS家族在茉莉花不同组织中及不同激素处理下的表达模式。结果显示,茉莉花TPS家族含有8个同时具有Terpene_synthase和Terpene_synthase_C结构域的家族成员,分别命名为JsTPS1-JsTPS8。茉莉花TPS成员的蛋白长度为163-844个氨基酸;分子量为19 103.6-96 887.4 kD,主要定位于细胞质和叶绿体中。系统进化树显示茉莉花TPS基因家族可以分为4个亚家族(a、b、e/f、g);qRT-PCR结果显示,JsTPS1JsTPS2JsTPS3JsTPS5JsTPS7基因在花瓣中高表达;经IAA、GA、SA和MeJA处理后JsTPS1JsTPS2JsTPS3JsTPS5JsTPS6JsTPS7的表达量受到不同程度的诱导表达。MeJA处理可显著诱导6个茉莉花TPS基因的上调表达,相对表达量最大值出现在4-9 h;4种激素的诱导作用由大到小分别为:MeJA>IAA>GA>SA。茉莉花含有8个TPS家族成员,茉莉花TPS基因主要在花瓣中高表达且与茉莉花香气形成关系密切,能够被IAA和MeJA诱导上调表达,GA和SA可能会抑制部分茉莉花TPS基因的表达。

关键词: 茉莉花, 萜类合成酶, 生物信息学, 表达分析

Abstract:

The objective of this work is to explore the role of terpenoid synthase(TPS)in the synthesis of terpenoid in jasmine and its expression under exogenous hormones treatments,which will lay a theoretical basis for further study of the function of J. sambac TPSJsTPS)family and its role in exogenous hormones stress. Based on the transcriptome data of jasmine in this study,bioinformatics was applied to identify and analyze the JsTPS family. Real-time fluorescence quantitative detection was to analyze the expressions of JsTPS family in different tissues and exogenous hormones treatments. The result showed that there were 8 TPS genes of having both Terpene_synthase and Terpene_synthase_C conserved domain in JsTPS family,named JsTPS1 - JsTPS8 respectively. The protein lengths of JsTPS genes ranged from 163 to 844 amino acids. The molecular weights of JsTPS genes ranged from 19 103.6 to 96 887.4 kD. And they were mainly located in cytoplasm and chloroplast. The JsTPS genes were divided into 4 subfamilies(a,b,e/f,and g)based on phylogenetic relationships. The analysis of qRT-PCR showed that the expressions of gene JsTPS1,JsTPS2,JsTPS3,JsTPS5,JsTPS6,and JsTPS7 were high in petals;also the expressions of the JsTPS1,JsTPS2,JsTPS3,JsTPS5,JsTPS6 and JsTPS7 were induced by IAA,GA,SA and MeJA. The MeJA significantly induced the expressions of 6 JsTPS genes,and the relative expression reached the maximum at 4-9 h. The induction from high to low was MeJA > IAA > GA > SA. J. sambac contained 8 TPS family members which were mainly expressed in petals and closely related to the formation of aroma in J. sambac. They were up-regulated under IAA and MeJA inductions. GA and SA treatments may inhibit the expressions of partial JsTPS genes.

Key words: Jasminum sambac, terpenoid synthase, bioinformatics, expression analysis