氟喹诺酮类抗生素及耐药基因污染控制的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2022-0497

摘要/Abstract

摘要：

氟喹诺酮类抗生素属于喹诺酮类抗生素,是一类人畜通用的抗生素。近年来,被广泛应用于人类和畜牧、水产等养殖业领域,然而其大量使用,造成在环境中的不断残留和累积,给自然环境和人类健康造成了较大威胁。现有研究表明,微生物降解是有效去除氟喹诺酮类抗生素残留污染的有效方法之一。本文总结和介绍了近年来氟喹诺酮类抗生素微生物降解单株菌和混合菌群、微生物降解酶、降解途径以及微生物降解氟喹诺酮类抗生素的实际应用,并对目前氟喹诺酮类抗生素微生物降解研究中存在的问题进行了分析,以及对未来氟喹诺酮类抗生素微生物降解研究的重点进行了探讨,以期为后续的研究提供参考。

关键词: 氟喹诺酮类抗生素, 降解菌, 降解酶, 降解途径

Abstract:

Fluoroquinolone antibiotics belong to quinolone antibiotics,is a kind of common antibiotics for human and livestock. In recent years,it has been widely used in human and animal husbandry,aquaculture and other aquaculture fields. However,its massive use has caused continuous residue and accumulation in the environment,posing a great threat to the natural environment and human health. Existing studies show that microbial degradation is one of the effective methods to remove the residual contamination of fluoroquinolone antibiotics. This paper summarized and introduced the practical application of fluoroquinolone antibiotics in microbial degradation of single strain and mixed bacteria,microbial degradation enzymes,degradation pathways and fluoroquinolone antibiotics in recent years,and analyzed the existing issues in the study of fluoroquinolone antibiotics microbial degradation. In addition,the paper also discussed the research focus of fluoroquinolone antibiotics microbial degradation in the future,aiming to provide reference for the follow-up research.

Key words: fluoroquinolone antibiotics, degradation bacteria, degradation enzyme, degradation pathway

李柳, 穆迎春, 刘璐, 张洪玉, 徐锦华, 杨臻, 乔璐, 宋金龙. 氟喹诺酮类抗生素及耐药基因污染控制的研究进展[J]. 生物技术通报, 2022, 38(9): 84-95.

LI Liu, MU Ying-chun, LIU Lu, ZHANG Hong-yu, XU Jin-hua, YANG Zhen, QIAO Lu, SONG Jin-long. Research Progress on Contamination Control of Fluoroquinolone Antibiotics and Drug Resistance Genes[J]. Biotechnology Bulletin, 2022, 38(9): 84-95.

图/表 3

参考文献 61

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氟喹诺酮类抗生素 Fluoroquinolone antibiotics	菌株 Strains	初始浓度 Initial concentration/（mg·L^-1）	降解率 Degradation rate /%	参考文献Reference
环丙沙星Ciprofloxacin	细菌Bacteria
环丙沙星Ciprofloxacin	Labrys portucalensis F11	1.15	85（28 d）	[9]
	Ochrobactrum sp. JOB	10	34（14 d）	[10]
	Thermus thermophiles C419	5	57（5 d）	[11]
	Bradyrhizobium sp. GLC_01	0.05	70（8 d）	[12]
	Bacillus sp. KM504129	5	74（14 d）	[13]
	Bacillus subtilius	5	70（14 d）	[13]
	Enterobacter sp. KM504128	5	94（14 d）	[13]
	Lactobacillus gesseri KM4055978	5	100（14 d）	[13]
	Micrococcus luteus	5	56（14 d）	[13]
	Tepidiphilus sp. M4	30	49（10 d）	[14]
	Lactobacillus reuteri WQ-Y1	4	65（1 d）	[15]
	Paraclostridium sp.	5	74（3 d）	[16]
	真菌Fungi
	Pestalotiopsis guepini P-8	100	67.7（18 d）	[17]
	Trametes versicolor ATCC42530	2	90（7 d）	[18]
	Trametes versicolor 617/93	10	100（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Panus tigrinus 577.79	10	60（14 d）	[19]
	Pleurotus ostreatus	1.50	95.07（14 d）	[20]
	Pycnoporus sanguineus CGMCC 5.815	10	98.5（2 d）	[21]
	Phanerochaete chrysosporium CGMCC 5.776	10	64.5（8 d）	[21]
	Trichoderma asperellum	0.20	81（13 d）	[22]
	Trichoderma harzianum	0.20	21（13 d）	[22]
诺氟沙星Norfloxacin	细菌Bacteria
	Labrys portucalensis F11	1.11	85（28 d）	[9]
	Mycobacterium sp.	100	75-98.4（7 d）	[23]
	Staphylococcus capraeNOR-36	5	92.6（10 d）	[24]
	Thermus thermophiles C419	5	63（72 h）	[10]
	Tepidiphilus sp. M4	30	51（10 d）	[14]
	Paraclostridium sp.	5	60（3 d）	[16]
	真菌Fungi
	Pestalotiopsis guepini P-8	100	68.9（18 d）	[17]
	Trametes versicolor ATCC42530	2	90（7 d）	[18]
	Trametes versicolor 617/93	10	85（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Pycnoporus sanguineus CGMCC 5.815	10	96.4（2 d）	[21]
	Phanerochaete chrysosporium CGMCC 5.776	10	73.2（8 d）	[21]
	Fusarium verticillioides MH045733	50	37.54（7 d）	[25]
	Fusarium solani MH045734	50	33.59（7 d）	[25]
	Aspergillus sydowii MH045735	50	15.67（7 d）	[25]
	Penicillium janthinellum MH045736	50	46.31（7 d）	[25]
氧氟沙星Ofloxacin	细菌Bacteria
	Thermus thermophiles C419	5	70（72 h）	[11]
	Tepidiphilus sp. M4	30	47（10 d）	[14]
	Paraclostridium sp.	5	68（3 d）	[16]
	Pseudomonas sp. F2	5	100	[26]
	Labrys portucalensis F11	0.45	34.6（28 d）	[27]
	Rhodococcus sp. FP1	0.45	39.3（28 d）	[27]
	真菌Fungi
	Trametes versicolor 617/93	10	100（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Trichoderma asperellum	0.20	44（13 d）	[22]
	Trichoderma harzianum	0.20	32（13 d）	[22]
恩诺沙星Enrofloxacin	细菌Bacteria
	Thermus thermophiles C419	5	74（72 h）	[11]
	Paraclostridium sp.	5	68（3 d）	[16]
	Enterococcus faecalis BSFL-1	100	63.2（96 h）	[28]
	Proteus mirabilis BSFL-2	100	57.2（96 h）	[28]
	Enterococcus faecalis BSFL-3	100	65.9（96 h）	[28]
	真菌Fungi
	Agrocybe praecox	——	——	[29]
	Coprinus macrocephalus	——	——	[29]
	Cyathus stercoreus	——	——	[29]

氟喹诺酮类抗生素 Fluoroquinolone antibiotics	菌株 Strains	初始浓度 Initial concentration/（mg·L^-1）	降解率 Degradation rate /%	参考文献Reference
环丙沙星Ciprofloxacin	细菌Bacteria
环丙沙星Ciprofloxacin	Labrys portucalensis F11	1.15	85（28 d）	[9]
	Ochrobactrum sp. JOB	10	34（14 d）	[10]
	Thermus thermophiles C419	5	57（5 d）	[11]
	Bradyrhizobium sp. GLC_01	0.05	70（8 d）	[12]
	Bacillus sp. KM504129	5	74（14 d）	[13]
	Bacillus subtilius	5	70（14 d）	[13]
	Enterobacter sp. KM504128	5	94（14 d）	[13]
	Lactobacillus gesseri KM4055978	5	100（14 d）	[13]
	Micrococcus luteus	5	56（14 d）	[13]
	Tepidiphilus sp. M4	30	49（10 d）	[14]
	Lactobacillus reuteri WQ-Y1	4	65（1 d）	[15]
	Paraclostridium sp.	5	74（3 d）	[16]
	真菌Fungi
	Pestalotiopsis guepini P-8	100	67.7（18 d）	[17]
	Trametes versicolor ATCC42530	2	90（7 d）	[18]
	Trametes versicolor 617/93	10	100（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Panus tigrinus 577.79	10	60（14 d）	[19]
	Pleurotus ostreatus	1.50	95.07（14 d）	[20]
	Pycnoporus sanguineus CGMCC 5.815	10	98.5（2 d）	[21]
	Phanerochaete chrysosporium CGMCC 5.776	10	64.5（8 d）	[21]
	Trichoderma asperellum	0.20	81（13 d）	[22]
	Trichoderma harzianum	0.20	21（13 d）	[22]
诺氟沙星Norfloxacin	细菌Bacteria
	Labrys portucalensis F11	1.11	85（28 d）	[9]
	Mycobacterium sp.	100	75-98.4（7 d）	[23]
	Staphylococcus capraeNOR-36	5	92.6（10 d）	[24]
	Thermus thermophiles C419	5	63（72 h）	[10]
	Tepidiphilus sp. M4	30	51（10 d）	[14]
	Paraclostridium sp.	5	60（3 d）	[16]
	真菌Fungi
	Pestalotiopsis guepini P-8	100	68.9（18 d）	[17]
	Trametes versicolor ATCC42530	2	90（7 d）	[18]
	Trametes versicolor 617/93	10	85（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Pycnoporus sanguineus CGMCC 5.815	10	96.4（2 d）	[21]
	Phanerochaete chrysosporium CGMCC 5.776	10	73.2（8 d）	[21]
	Fusarium verticillioides MH045733	50	37.54（7 d）	[25]
	Fusarium solani MH045734	50	33.59（7 d）	[25]
	Aspergillus sydowii MH045735	50	15.67（7 d）	[25]
	Penicillium janthinellum MH045736	50	46.31（7 d）	[25]
氧氟沙星Ofloxacin	细菌Bacteria
	Thermus thermophiles C419	5	70（72 h）	[11]
	Tepidiphilus sp. M4	30	47（10 d）	[14]
	Paraclostridium sp.	5	68（3 d）	[16]
	Pseudomonas sp. F2	5	100	[26]
	Labrys portucalensis F11	0.45	34.6（28 d）	[27]
	Rhodococcus sp. FP1	0.45	39.3（28 d）	[27]
	真菌Fungi
	Trametes versicolor 617/93	10	100（14 d）	[19]
	Irpex lacteus 617/93	10	100（10 d）	[19]
	Trichoderma asperellum	0.20	44（13 d）	[22]
	Trichoderma harzianum	0.20	32（13 d）	[22]
恩诺沙星Enrofloxacin	细菌Bacteria
	Thermus thermophiles C419	5	74（72 h）	[11]
	Paraclostridium sp.	5	68（3 d）	[16]
	Enterococcus faecalis BSFL-1	100	63.2（96 h）	[28]
	Proteus mirabilis BSFL-2	100	57.2（96 h）	[28]
	Enterococcus faecalis BSFL-3	100	65.9（96 h）	[28]
	真菌Fungi
	Agrocybe praecox	——	——	[29]
	Coprinus macrocephalus	——	——	[29]
	Cyathus stercoreus	——	——	[29]