WOX家族基因调控植物生长发育和非生物胁迫响应的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2022-1098

摘要/Abstract

摘要：

WOX（WUSCHEL-related homeobox）家族是植物特有的一类转录因子家族，其含有由65-66个氨基酸残基组成的同源异型结构域（Homeodomain，HD）。植物WOX家族成员通过在转录水平上调控靶基因表达，从而参与植物的生长发育和对非生物胁迫的响应等重要生物过程。综述了植物WOX家族成员的分类、结构特征，重点介绍了其在植物生长发育（根、茎、叶、花、果实、种子、胚胎）的调控及植物响应非生物（干旱、盐、冷）胁迫方面的功能研究进展，并对研究WOX转录因子的意义及有待解决的问题进行了展望，旨在为进一步研究WOX家族基因的功能提供参考。

关键词: 转录因子, WOX, 生长发育, 非生物胁迫

Abstract:

The WOX（WUSCHEL-related homeobox）proteins comprise a plant-specific transcription factor family, which contain a DNA-binding homeodomain（HD）consisting of 65-66 amino acid residues. WOX family members are involved in important biological processes such as plant growth and development and responses to abiotic stress by regulating the expressions of target genes at the transcriptional level. This article reviews the classification and structural characteristics of plant WOX family members, and focusing on the functional research progress in the regulation of plant growth and development（such as root, stem, leaf, flower, fruit, seed, and embryo）and plant responses to abiotic（including drought, salt, and cold）stresses. The significance of studying of WOX transcription factors and the issues to be solved are also prospected, aiming to provide a reference for further research on the function of WOX family genes.

Key words: transcription factors, WOX, growth and development, abiotic stress

冯珊珊, 王璐, 周益, 王幼平, 方玉洁. WOX家族基因调控植物生长发育和非生物胁迫响应的研究进展[J]. 生物技术通报, 2023, 39(5): 1-13.

FENG Shan-shan, WANG Lu, ZHOU Yi, WANG You-ping, FANG Yu-jie. Research Progresses on WOX Family Genes in Regulating Plant Development and Abiotic Stress Response[J]. Biotechnology Bulletin, 2023, 39(5): 1-13.

图/表 5

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基因 Genes	物种 Species	功能 Function	参考文献 Reference
WUS	杨树，小麦 P. tomentosa, and T. aestivum	初生根和侧根发育，植物株型 Primary root and lateral root development，and plant type	[18,23,33]
WOX1	拟南芥，小麦，番茄 A. thaliana, T. aestivum, and L. esculentum	叶片的侧向生长和形状结构 Lateral growth and shape structure of leaves	[37,43,48⇓⇓-51]
WOX2	拟南芥，挪威云杉，海岸松 A. thaliana, P. abies, and P. pinaster	胚胎发育 Embryonic development	[61-62]
WOX3	柳枝稷，拟南芥，水稻，玉米 P. virgatum, A. thaliana, O. sativa, and Z. mays	叶片发育和形态建成 Leaf development and morphogenesis	[44⇓⇓-47]
WOX4	拟南芥，水稻 A. thaliana and O. sativa	促进形成层，初生根的分化 Promote differentiation of cambium and primary root	[17,20]
WOX5	杨树P. tomentosa	初生根发育Primary root development	[22]
WOX6	拟南芥A. thaliana	调控胚珠和种子发育Regulation of ovule and seed development	[55-56]
WOX7	拟南芥A. thaliana	侧根发育Lateral root development	[16]
WOX8	拟南芥，水稻 A. thaliana and O. sativa	胚胎发育，促进主茎生长 Embryonic development, promoting the growth of main stem	[58⇓-60]
WOX9	拟南芥，烟草，矮牵牛，番茄 A. thaliana, N. tabacum, P. hybrid, and L. esculentum	胚胎发育，根系发育，SAM，花分生组织发育 Embryonic development, root development, SAM, and floral meristem development	[24,43,63]
WOX10	水稻O. sativa	促进初生根的分化Promote differentiation of primary roots	[21]
WOX11	水稻，人参，杨树 O. sativa, P. ginseng, and P. tomentosa	促进根，茎，芽发育 Promote the development of roots, stems and buds	[19,24,32]
WOX12	拟南芥，杨树A. thaliana and P. tomentosa	根系发育Root development	[66]
WOX13	拟南芥A. thaliana	促进器官重生，花器官形成 Promote organ regeneration and flower organ formation	[15-16,30,53]
WOX14	拟南芥A. thaliana	根和花的发育Development of roots and flowers	[15,28]

基因 Genes	物种 Species	功能 Function	参考文献 Reference
WUS	杨树，小麦 P. tomentosa, and T. aestivum	初生根和侧根发育，植物株型 Primary root and lateral root development，and plant type	[18,23,33]
WOX1	拟南芥，小麦，番茄 A. thaliana, T. aestivum, and L. esculentum	叶片的侧向生长和形状结构 Lateral growth and shape structure of leaves	[37,43,48⇓⇓-51]
WOX2	拟南芥，挪威云杉，海岸松 A. thaliana, P. abies, and P. pinaster	胚胎发育 Embryonic development	[61-62]
WOX3	柳枝稷，拟南芥，水稻，玉米 P. virgatum, A. thaliana, O. sativa, and Z. mays	叶片发育和形态建成 Leaf development and morphogenesis	[44⇓⇓-47]
WOX4	拟南芥，水稻 A. thaliana and O. sativa	促进形成层，初生根的分化 Promote differentiation of cambium and primary root	[17,20]
WOX5	杨树P. tomentosa	初生根发育Primary root development	[22]
WOX6	拟南芥A. thaliana	调控胚珠和种子发育Regulation of ovule and seed development	[55-56]
WOX7	拟南芥A. thaliana	侧根发育Lateral root development	[16]
WOX8	拟南芥，水稻 A. thaliana and O. sativa	胚胎发育，促进主茎生长 Embryonic development, promoting the growth of main stem	[58⇓-60]
WOX9	拟南芥，烟草，矮牵牛，番茄 A. thaliana, N. tabacum, P. hybrid, and L. esculentum	胚胎发育，根系发育，SAM，花分生组织发育 Embryonic development, root development, SAM, and floral meristem development	[24,43,63]
WOX10	水稻O. sativa	促进初生根的分化Promote differentiation of primary roots	[21]
WOX11	水稻，人参，杨树 O. sativa, P. ginseng, and P. tomentosa	促进根，茎，芽发育 Promote the development of roots, stems and buds	[19,24,32]
WOX12	拟南芥，杨树A. thaliana and P. tomentosa	根系发育Root development	[66]
WOX13	拟南芥A. thaliana	促进器官重生，花器官形成 Promote organ regeneration and flower organ formation	[15-16,30,53]
WOX14	拟南芥A. thaliana	根和花的发育Development of roots and flowers	[15,28]

基因 Genes	物种 Species	研究方法 Methods	胁迫类型 Stress type	生物学功能 Biological function	参考文献 Ref.
OsWOX13	水稻 O. sativa	过表达 Overexpression	干旱胁迫 Drought	利用rab21启动子驱动OsWOX13过表达导致水稻耐旱性增强 Overexpression of OsWOX13 driven by rab21 promoter leads to enhanced drought tolerance in rice	[53]
JcWOX5	麻风树基因导入水稻 O. sativa and Jatro-pha curcas	过表达 Overexpression	干旱胁迫 Drought	JcWOX5负调控拟南芥的干旱反应 JcWOX5 negatively regulates drought response	[64]
OsWOX10，QHB/OsWOX5	水稻 O. sativa	CRISPR CRISPR-qhb	干旱胁迫 Drought	QHB和OsWOX10控制土壤条件下轻度干旱诱导LR发育 LR development induced by mild drought in soil controlled by QHB and OsWOX10	[21]
GhWOX4	棉花，拟南芥中异位表达 G. Hirsutum and A. thaliana	过表达，病毒诱导基因沉默（VIGS）技术 Overexpression，Virus induced gene silencing technology	干旱胁迫 Drought	GhWOX4正调控棉花耐旱性 GhWOX4 positively regulated drought tolerance	[65]
PagWOX11/12a	杂交白杨84K Pop-ulus alba×Populus glandulosa	过表达 Overexpression	干旱胁迫 Drought	PagWOX11/12a通过促进根系伸长，生物量增长，ROS清除相关基因的表达来调节ROS水平，从而提高植物耐旱性 PagWOX11/12a regulates ROS level by promoting root elongation, biomass growth and ROS clearance related gene expression, thus improving plant drought tolerance	[66-67]
WOX6	拟南芥 A. thaliana	hos9-1突变体 hos9-1 mutant	冷胁迫 Freezing	HOS9正调控拟南芥对冷胁迫的耐受性 HOS9 positively regulated freezing tolerance	[68]
WOX11	水稻 O. sativa	过表达 Overexpression	营养胁迫 Nutritional	WOX11基因能够控制根系发育并提高水稻对缺钾的耐受性 WOX11 can control root development and improve tolerance of rice to potassium deficiency	[69]
PagWOX11/12a	杨树 P. tomentosa	过表达 Overexpression	盐胁迫 Salt	PagWOX11/12a通过激活PagCYP736A12基因的表达从而正调控杨树的耐盐性 PagWOX11/12a regulates salt tolerance of poplar by activating the expression of PagCYP736A12 gene	[70]

基因 Genes	物种 Species	研究方法 Methods	胁迫类型 Stress type	生物学功能 Biological function	参考文献 Ref.
OsWOX13	水稻 O. sativa	过表达 Overexpression	干旱胁迫 Drought	利用rab21启动子驱动OsWOX13过表达导致水稻耐旱性增强 Overexpression of OsWOX13 driven by rab21 promoter leads to enhanced drought tolerance in rice	[53]
JcWOX5	麻风树基因导入水稻 O. sativa and Jatro-pha curcas	过表达 Overexpression	干旱胁迫 Drought	JcWOX5负调控拟南芥的干旱反应 JcWOX5 negatively regulates drought response	[64]
OsWOX10，QHB/OsWOX5	水稻 O. sativa	CRISPR CRISPR-qhb	干旱胁迫 Drought	QHB和OsWOX10控制土壤条件下轻度干旱诱导LR发育 LR development induced by mild drought in soil controlled by QHB and OsWOX10	[21]
GhWOX4	棉花，拟南芥中异位表达 G. Hirsutum and A. thaliana	过表达，病毒诱导基因沉默（VIGS）技术 Overexpression，Virus induced gene silencing technology	干旱胁迫 Drought	GhWOX4正调控棉花耐旱性 GhWOX4 positively regulated drought tolerance	[65]
PagWOX11/12a	杂交白杨84K Pop-ulus alba×Populus glandulosa	过表达 Overexpression	干旱胁迫 Drought	PagWOX11/12a通过促进根系伸长，生物量增长，ROS清除相关基因的表达来调节ROS水平，从而提高植物耐旱性 PagWOX11/12a regulates ROS level by promoting root elongation, biomass growth and ROS clearance related gene expression, thus improving plant drought tolerance	[66-67]
WOX6	拟南芥 A. thaliana	hos9-1突变体 hos9-1 mutant	冷胁迫 Freezing	HOS9正调控拟南芥对冷胁迫的耐受性 HOS9 positively regulated freezing tolerance	[68]
WOX11	水稻 O. sativa	过表达 Overexpression	营养胁迫 Nutritional	WOX11基因能够控制根系发育并提高水稻对缺钾的耐受性 WOX11 can control root development and improve tolerance of rice to potassium deficiency	[69]
PagWOX11/12a	杨树 P. tomentosa	过表达 Overexpression	盐胁迫 Salt	PagWOX11/12a通过激活PagCYP736A12基因的表达从而正调控杨树的耐盐性 PagWOX11/12a regulates salt tolerance of poplar by activating the expression of PagCYP736A12 gene	[70]