多组学分析不同光质下血叶兰类胡萝卜素的差异积累

doi:10.13560/j.cnki.biotech.bull.1985.2025-0379

摘要/Abstract

摘要：

目的探究不同光质对血叶兰叶片类胡萝卜素类化合物积累的影响及其分子机制，为血叶兰规范化栽培提供理论参考。方法以血叶兰‘闽热圆帅’的叶片为实验材料，分析其在白光（W）、蓝光（B）、黄光（Y）处理下的代谢与转录调控机制。利用液相色谱‒质谱联用技术（LC-MS/MS）和高通量转录组测序（RNA-seq）技术，分别获取代谢组和转录组数据。以白光组为对照，分析蓝光和黄光对类胡萝卜素含量、相关代谢物及基因表达的影响。通过RT-qPCR验证8个类胡萝卜素合成关键基因的表达模式。结果蓝光处理显著提升血叶兰叶片中总类胡萝卜素含量，而黄光处理未引起显著变化。代谢组学分析鉴定出23个与类胡萝卜素合成相关的差异代谢物，包括黄原酸、脱落酸醇、独脚金内酯ABC-环和链孢霉黄素等。转录组学分析发现9个差异表达的代谢酶基因（如CrtZ、Z-ISO、PSY等）及6个关键转录因子（ERF002、ERF059、ERF066等），这些转录因子可能通过响应赤霉素、茉莉酸甲酯、生长素、水杨酸和脱落酸信号调控类胡萝卜素合成。RT-qPCR验证证实8个关键基因在类胡萝卜素代谢调控中发挥潜在作用。结论蓝光处理下，血叶兰叶片中ERF和bZIP家族转录因子通过与赤霉素、茉莉酸甲酯、生长素、水杨酸和脱落酸相关的顺式作用元件结合，调控下游酶基因的表达，从而促进类胡萝卜素相关代谢物的显著积累。

关键词: 血叶兰, 光质, 类胡萝卜素, 转录组, 代谢组, 转录因子, 植物激素元件

Abstract:

Objective This study is aimed to investigate the effects of different light qualities on the accumulation of carotenoid compounds in the leaves of Ludisia discolor and their molecular mechanisms, which may provide a theoretical reference for the standardized cultivation of this medicinal plant. Method This study used Ludisia discolor ‘Minre Yuanshuai’ to analyze the metabolic and transcriptional regulatory mechanisms under white light (W), blue light (B), and yellow light (Y) treatments. Liquid chromatography-mass spectrometry (LC-MS/MS) and high-throughput transcriptome sequencing (RNA-seq) technologies were utilized to obtain metabolomic and transcriptomic data, respectively. The white light group was used as a control to analyze the effects of blue and yellow light on carotenoid content, related metabolites and gene expression. RT-qPCR was adapted to verify the expression patterns of eight key genes closely related to carotenoid synthesis. Result The blue light treatment significantly increased total carotenoid levels in L. discolor leaves, whereas yellow light treatment caused no substantial changes. The metabolomic results implied that there were 23 differential metabolites related to carotenoid synthesis, including xanthoxic acid, abscisic alcohol, strigolactone ABC-rings, and neurosporaxanthin. Transcriptome analysis revealed nine differentially expressed metabolic enzyme genes (such as CrtZ, Z-ISO, PSY) and six key transcription factors (ERF002, ERF059, ERF066), which may regulate carotenoid synthesis in response to gibberellin, methyl jasmonate, auxin, salicylic acid, and abscisic acid signals. RT-qPCR confirmed that the eight key genes play a potential role in the regulation of carotenoid metabolism. Conclusion Under blue light treatment, ERF and bZIP transcription factors in L. discolor leaves regulate the expressions of downstream enzyme genes by binding to cis-acting elements related to gibberellins, methyl jasmonate, growth hormone, salicylic acid, and abscisic acid, leading to significant accumulation of carotenoid-related metabolites.

Key words: Ludisia discolor, light quality, carotenoids, transcriptome, metabolome, transcription factors, plant hormone elements

张雨轩, 张诗怡, 陈会芳, 蔡坤秀, 李晨烨, 杨俊杰, 郑涛, 仇明月, 杨有思媛, 陈莹. 多组学分析不同光质下血叶兰类胡萝卜素的差异积累[J]. 生物技术通报, 2025, 41(10): 98-109.

ZHANG Yu-xuan, ZHANG Shi-yi, CHEN Hui-fang, CAI Kun-xiu, LI Chen-ye, YANG Jun-jie, ZHENG Tao, QIU Ming-yue, YANG You-si-yuan, CHEN Ying. Differential Accumulation of Carotenoids in Ludisia discolor under Different Light Qualities Based on Multiomics[J]. Biotechnology Bulletin, 2025, 41(10): 98-109.

图/表 12

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基因名称 Gene name	基因ID Gene ID	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）
CAC	Reference gene	TCCGACAAATTGGAGGTTGC	TGCTGCTGACAACAATCACG
PSY-1	TRINITY_DN3430_c0_g1	CGAAGATGCCACAGGGAAGT	TTCTGTCAGCTGCTGTGAGG
NCED	TRINITY_DN3795_c2_g1	ATCCATTGGAAGGGTTGCCC	ACAGGCCTGTTTTAGAGGACC
ZEP	TRINITY_DN761_c0_g1	AGGCAAAGCTTGAAATGCGG	AAGCTGCGGCACTGTATCTT
CrtZ	TRINITY_DN11602_c0_g1	CTGGCAATCCGGATCCAACT	TCCGACGTCAACGATCGTTT
Z-ISO	TRINITY_DN19139_c0_g1	TTGGGGCTAGAAGTTTGGGG	GCATGGTGCGAACTATAAGGAC
CYP707A-1	TRINITY_DN5732_c0_g1	TCTCCATGATCCGACTGCAC	GACCGAAAGTCGAGAGTCTCA
LUT1	TRINITY_DN5391_c0_g1	TCAATCTCCCCTGACCGTCT	CTTGAACCAGACAGCCCCTT
VDE	TRINITY_DN10121_c0_g1	AGCAAACGCAAACTCCCCTA	ACTAACCTGCATGCCCCAAA

基因名称 Gene name	基因ID Gene ID	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）
CAC	Reference gene	TCCGACAAATTGGAGGTTGC	TGCTGCTGACAACAATCACG
PSY-1	TRINITY_DN3430_c0_g1	CGAAGATGCCACAGGGAAGT	TTCTGTCAGCTGCTGTGAGG
NCED	TRINITY_DN3795_c2_g1	ATCCATTGGAAGGGTTGCCC	ACAGGCCTGTTTTAGAGGACC
ZEP	TRINITY_DN761_c0_g1	AGGCAAAGCTTGAAATGCGG	AAGCTGCGGCACTGTATCTT
CrtZ	TRINITY_DN11602_c0_g1	CTGGCAATCCGGATCCAACT	TCCGACGTCAACGATCGTTT
Z-ISO	TRINITY_DN19139_c0_g1	TTGGGGCTAGAAGTTTGGGG	GCATGGTGCGAACTATAAGGAC
CYP707A-1	TRINITY_DN5732_c0_g1	TCTCCATGATCCGACTGCAC	GACCGAAAGTCGAGAGTCTCA
LUT1	TRINITY_DN5391_c0_g1	TCAATCTCCCCTGACCGTCT	CTTGAACCAGACAGCCCCTT
VDE	TRINITY_DN10121_c0_g1	AGCAAACGCAAACTCCCCTA	ACTAACCTGCATGCCCCAAA

序号No.	名称 Name	分子式 Formula	KEGG注释 KEGG_annotation
1	黄原酸 Xanthoxic acid	C₁₅H₂₂O₄	C13454
2	反式法呢基二磷酸酯 Trans，trans-Farnesyl diphosphate	C₁₅H₂₈O₇P₂	C00448
3	脱落酸醇 Abscisic alcohol	C₁₅H₂₂O₃	C13456
4	独脚金内酯ABC-环 Strigolactone ABC-rings	C₁₄H₁₈O₃	C18036
5	黄酮糖苷 Rhodopinal glucoside	C₄₆H₆₆O₇	C16271
6	螺旋黄质 Spheroidenone	C₄₁H₅₈O₂	C15903
7	3'，4'-二氢紫菌红醇 3'，4'-Dihydrorhodovibrin	C₄₁H₆₂O₂	C15887
8	前八氢番茄红素二磷酸酯 Prephytoene diphosphate	C₄₀H₆₈O₇P₂	C03427
9	羟基螺环菌素 Hydroxyspirilloxanthin	C₄₁H₅₈O₂	C15879
10	链孢霉黄素 Neurosporaxanthin	C₃₅H₄₆O₂	C08607
11	氯黄质 Chloroxanthin	C₄₀H₆₀O	C15892
12	黄质醛 Xanthoxin	C₁₅H₂₂O₃	C13453
13	脱落酸葡萄糖酯 Abscisic acid glucose ester	C₂₁H₃₀O₉	C15970
14	脱落酸 Abscisate	C₁₅H₂₀O₄	C06082
15	独角金醇 Strigol	C₁₉H₂₂O₆	C09190
16	8'-羟基脱落物 8'-Hydroxyabscisate	C₁₅H₂₀O₅	C15514
17	螺旋黄质 Spirilloxanthin	C₄₂H₆₀O₂	C15881
18	玉米黄质二葡萄糖苷 Zeaxanthin diglucoside	C₅₂H₇₆O₁₂	C15969
19	（2'S）-脱氧麦芽醇2'-（2，4-二-O-甲基-α-L-葡萄糖苷）（2'S）-Deoxymyxol 2'-（2，4-di-O-methyl-alpha-L-fucoside）	C₄₈H₇₀O₆	C15935
20	脱落醛 Abscisic aldehyde	C₁₅H₂₀O₃	C13455
21	叶黄素 Lutein	C₄₀H₅₆O₂	C08601
22	二氢相酸 Dihydrophaseic acid	C₁₅H₂₂O₅	C15971
23	视紫红醛 Rhodopinal	C₄₀H₅₆O₂	C16270

序号No.	名称 Name	分子式 Formula	KEGG注释 KEGG_annotation
1	黄原酸 Xanthoxic acid	C₁₅H₂₂O₄	C13454
2	反式法呢基二磷酸酯 Trans，trans-Farnesyl diphosphate	C₁₅H₂₈O₇P₂	C00448
3	脱落酸醇 Abscisic alcohol	C₁₅H₂₂O₃	C13456
4	独脚金内酯ABC-环 Strigolactone ABC-rings	C₁₄H₁₈O₃	C18036
5	黄酮糖苷 Rhodopinal glucoside	C₄₆H₆₆O₇	C16271
6	螺旋黄质 Spheroidenone	C₄₁H₅₈O₂	C15903
7	3'，4'-二氢紫菌红醇 3'，4'-Dihydrorhodovibrin	C₄₁H₆₂O₂	C15887
8	前八氢番茄红素二磷酸酯 Prephytoene diphosphate	C₄₀H₆₈O₇P₂	C03427
9	羟基螺环菌素 Hydroxyspirilloxanthin	C₄₁H₅₈O₂	C15879
10	链孢霉黄素 Neurosporaxanthin	C₃₅H₄₆O₂	C08607
11	氯黄质 Chloroxanthin	C₄₀H₆₀O	C15892
12	黄质醛 Xanthoxin	C₁₅H₂₂O₃	C13453
13	脱落酸葡萄糖酯 Abscisic acid glucose ester	C₂₁H₃₀O₉	C15970
14	脱落酸 Abscisate	C₁₅H₂₀O₄	C06082
15	独角金醇 Strigol	C₁₉H₂₂O₆	C09190
16	8'-羟基脱落物 8'-Hydroxyabscisate	C₁₅H₂₀O₅	C15514
17	螺旋黄质 Spirilloxanthin	C₄₂H₆₀O₂	C15881
18	玉米黄质二葡萄糖苷 Zeaxanthin diglucoside	C₅₂H₇₆O₁₂	C15969
19	（2'S）-脱氧麦芽醇2'-（2，4-二-O-甲基-α-L-葡萄糖苷）（2'S）-Deoxymyxol 2'-（2，4-di-O-methyl-alpha-L-fucoside）	C₄₈H₇₀O₆	C15935
20	脱落醛 Abscisic aldehyde	C₁₅H₂₀O₃	C13455
21	叶黄素 Lutein	C₄₀H₅₆O₂	C08601
22	二氢相酸 Dihydrophaseic acid	C₁₅H₂₂O₅	C15971
23	视紫红醛 Rhodopinal	C₄₀H₅₆O₂	C16270

基因名称 Gene name	基因ID Gene ID	酶编号 Corresponding enzyme	KO号 Enzyme KO
CrtZ	TRINITY_DN11602_c0_g1	［EC：1.14.15.24］	K15746
Z-ISO	TRINITY_DN19139_c0_g1	［EC：5.2.1.12］	K15744
PSY-1	TRINITY_DN3430_c0_g1	［EC：2.5.1.32］	K02291
PSY-2	TRINITY_DN3430_c0_g2	［EC：2.5.1.32］	K02291
CYP707A-1	TRINITY_DN5732_c0_g1	［EC：1.14.14.137］	K09843
CYP707A-2	TRINITY_DN10055_c0_g1	［EC：1.14.14.137］	K09843
LUT1	TRINITY_DN5391_c0_g1	［EC：1.14.14.158］	K09837
VDE	TRINITY_DN10121_c0_g1	［EC：1.23.5.1］	K09839
NCED	TRINITY_DN3795_c2_g1	［EC：1.13.11.51］	K09840
ZDS	TRINITY_DN25937_c0_g1	［EC：1.3.5.6］	K00514
ZEP	TRINITY_DN761_c0_g1	［EC：1.14.15.21］	K09838