香蕉泛素结合酶基因MaUCE2在非生物胁迫下的表达分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 77-80.

香蕉泛素结合酶基因MaUCE2在非生物胁迫下的表达分析

王安邦^{1, 3}, 金志强², 刘菊华³, 贾彩红³, 张建斌³, , 苗红霞³, 徐碧玉^{2, 3}

(1. 海南大学农学院，海口 571101；2. 中国热带农业科学院海口实验站香蕉研究所，海口 571101；3. 中国热带农业科学院热带生物技术研究所，海口 571101)

收稿日期:2012-12-17 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:王安邦，男，硕士研究生，研究方向：种质资源；E-mail：wanganbang011@126.com
基金资助:
国家现代香蕉产业技术体系(CARS-32), “十二五”农村领域国家科技计划课题(2011AA10020605)

Expression Analysis of a Banana Ubiquitin-conjugating Enzyme Gene MaUCE2 Under Abiotic Stress

Wang Anbang^{1, 3}, Jin Zhiqiang², Liu Juhua³, Jia Caihong³, Zhang Jianbin³, Miao Hongxia³, Xu Biyu^{2, 3}

(1. Department of Agriculture，Hainan University，Haikou 571101 ；2. Haikou Experimental Station，Chinese Academy of Tropical Agricultural Science，Haikou 571101；3. Institute of Tropical Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Science，Haikou 571101)

Received:2012-12-17 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24
About author:徐碧玉，女，研究员，博士生导师，研究方向：香蕉生物技术；E-mail：biyuxu@126.com

摘要/Abstract

摘要： 为了研究泛素结合酶(ubiquitin-conjugating enzyme)E2和植物抗逆性的关系，对香蕉苗进行了非生物胁迫(干旱、低温、盐胁迫)处理，利用荧光定量PCR技术分析了香蕉泛素结合酶基因MaUCE2在不同胁迫条件下的表达特性。结果表明，MaUCE2表达量随着干旱程度的加重而逐步上升，在高度干旱胁迫下MaUCE2的表达量最高；低温胁迫可以诱导MaUCE2的表达，其表达量随着温度的降低而逐步升高，当温度降低到5℃时，MaUCE2的表达量最高；而在盐胁迫下，MaUCE2的表达量与对照相比略有上升，上述结果表明MaUCE2基因表达受非生物胁迫的诱导。

关键词: 香蕉, 泛素结合酶, 非生物胁迫, 干旱, 盐胁迫, 低温

Abstract: In order to study the relationship between ubiquitin-conjugating enzyme E2 and stress resistance of plants, the banana seedings were treated with abiotic stress(drought, low temperature and salt stress). The expression levels of MaUCE2 in banana root under different stresses were analyzed by real-time qPCR. The results showed that MaUCE2 expression increased with the drought degree. In addition, the expression levels of MaUCE2 under severe drought is the highest. MaUCE2 expression is induced by low temperature stress, which increased with the decrease of the temperature. When the temperature down to 5℃, the MaUCE2 expression reached to the highest level. The expression levels of MaUCE2 under salt stress increased slightly compared with the control group. These results indicate that MaUCE2 was induced by abiotic stresses and might provide a foundation for the further research and application of biological function.

Key words: Banana, Ubiquitin conjugating enzyme, Abiotic stress Drought, Salt stress, Low temperature

王安邦, 金志强, 刘菊华, 贾彩红, 张建斌, , 苗红霞, 徐碧玉. 香蕉泛素结合酶基因MaUCE2在非生物胁迫下的表达分析[J]. 生物技术通报, 2013, 0(5): 77-80.

Wang Anbang, Jin Zhiqiang, Liu Juhua, Jia Caihong, Zhang Jianbin, Miao Hongxia, Xu Biyu^{2, 3}. Expression Analysis of a Banana Ubiquitin-conjugating Enzyme Gene MaUCE2 Under Abiotic Stress[J]. Biotechnology Bulletin, 2013, 0(5): 77-80.

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(责任编辑李楠)