Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (1): 86-91.doi: 10.13560/j.cnki.biotech.bull.1985.2015.01.013

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Physiological and Biochemical Analysis of Young Shoot Purple-related and Gene Expression of Key Enzymes in Tea Plant(Camellia sinensis)

Zhou Qiongqiong, Sun Weijiang1,2   

  1. (1. College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002;2. Anxi Tea College,Fujian Agriculture and Forestry University,Fuzhou 350002)
  • Received:2014-06-15 Online:2015-01-09 Published:2015-01-10

Abstract: The young purple shoots of tea plant(Camellia sinensis)were compared to mature green leaves based on the biochemical and molecular level. Results showed that the content of tea polyphenols,catechins,caffeine and anthocyanins in the tender purple leaves were significantly higher than those in mature green leaves,while the content of chlorophyll and chlorophyll a,b,carotenoids were significantly lower than those mature green leaves. Q-PCR analysis indicated that the key genes of PAL、C4H、CHS、CHI、F3H、F3'H、F3'5'H、DFR and ANS in the anthocyanin biosynthesis were up-regulated in the tender purple leaves,which may contribute to the synthesis of anthocyanins and the color of the young shoots.

Key words: tea plant, purple leaves, physical and chemical analysis, gene expression