Advances and Applications on Methodology of 16S rRNA Sequencing in Gut Microbiota Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.010

Abstract

Abstract: 16S rRNA sequencing is one of the high-throughput-sequencing-based methods used in gut microbiota analysis. Almost all the bacterial species in gut microbiota can be quantified through 16S rRNA sequencing, which has made this method into the mainstream. Two issues are very important in the application of 16S rRNA sequencing：sequencing strategy and bioinformatic analysis. In this review, three aspects of the sequencing strategy, including sequencing platform, sequencing region, and data size were discussed. While on bioinformatic analysis, the advance in sequences cluster and annotation, microbiota structure analysis, key taxa screening and functional analysis were reviewed here.

Key words: intestinal microbiota, 16S rRNA, high-throughput sequencing

Li Dongping, Guo Mingzhang, Xu Wentao. Advances and Applications on Methodology of 16S rRNA Sequencing in Gut Microbiota Analysis[J]. Biotechnology Bulletin, 2015, 31(2): 71-77.

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