The Comparsion of Bacillus Species Classification Based on Fatty Acid and 16S rRNA Gene

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.021

Abstract

Abstract: In order to explore the application of fatty acid composition in taxonomy of Bacillus species, the fatty acid constitutions of 25 strains were detected by Microbial Identification System(MIDI). The clusters of fatty acid profiles and 16S rRNA gene sequences were analyzed by SPPS16.0 and Mega4, respectively. The results showed that phylogeny analysis based on the fatty acid biomarkers could not only fully reflect the relationships among the Bacillus species, but also group the Bacillus species according to the biological characteristics. However, 16S rRNA phylogeny only perfectly showed the relationships among the species. For example, four species growing well under the alkaline conditions and three species round spore-forming were clustered together, respectively. Result showed that Bacillus species can be clustered together not only according to the related ship, but also classified by the biological characteristics.

Key words: Bacillus, fatty acid profile, 16S rRNA, phylogeny

Liu Guohong, Liu Bo, Lin Yingzhi, Tang Jianyang. The Comparsion of Bacillus Species Classification Based on Fatty Acid and 16S rRNA Gene[J]. Biotechnology Bulletin, 2015, 31(3): 146-153.

References

[1] 刘波, 朱昌雄. 微生物发酵床零污染养猪技术的研究与应用[M]. 北京：中国农业科学技术出版社, 2009.
[2] 车建美, 郑雪芳, 刘波, 等. 短短芽胞杆菌FJAT-0809-GLX 菌剂的制备及其对枇杷保鲜效果的研究[J]. 保鲜与加工, 2011, 11(5)：6-9.
[3] 刘波,刘国红,林乃铨. 基于脂肪酸生物标记的芽胞杆菌属种类的系统发育研究[J]. 微生物学报, 2014, 54(2)：139-158.
[4] 陶天申, 杨瑞馥, 东秀珠. 原核生物系统学[M]. 北京：化学工业出版社, 2007.
[5] Abel K, Deschmertzing H, Peterson JI. Classification of microorgan-isms by analysis of chemical composition. I. Feasibility of utilizing gas chromatography[J]. J Bacteriol, 1963, 85(5)：1039-1044.
[6] Kaneda T. Iso- and anteiso-fatty acids in bacteria：biosynthesis, function, and taxonomic significance[J]. Microbiological Reviews, 1991, 55(2)：288-302.
[7] K?mpfer P. Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species[J]. Syst Appl Microbiol, 1994, 17：86-98.
[8] 张晓霞, 王直强, 李世贵, 等. 脂肪酸组分分析在不动杆菌鉴定中的应用[J]. 生物技术通报, 2009(6)：150-153.
[9] 林营志, 刘波, 张秋芳, 等. 土壤微生物群落磷脂脂肪酸生物标记分析程序PLFAEco[J]. 中国农学通报, 2009, 25(14)：286-290.
[10] Kim OS, Cho YJ, Lee K, et al. Introducing EzTaxon-e：a prokary-otic 16S rRNA gene sequence database with phylotypes that represent uncultured species[J]. Int J Syst Evol Microbiol, 2012, 62：716-721.
[11] Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL_X windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Res, 1997, 25：4876-4882.
[12] Jukes TH, Cantor CR. Evolution of protein molecules[M]// Munro HN. Mammalian Protein Metabolism. New York：Academic Press, 1969：21-132.
[13] Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method[J]. Proceedings of the National Academy of Sciences(USA), 2004, 101：11030-11035.
[14] Saitou N, Nei M. The neighbor-joining method：A new method for reconstructing phylogenetic trees[J]. Mol Biol Evol, 1987, 4：406-425.
[15] Felsenstein J. Confidence limits on phylogenies：an approach using the bootstrap[J]. Evolution, 1985, 40：783-791.
[16] Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids[J]. USFCC News, 1990, 20：16.
[17] Whittaker P, Day JB, Curtis SK, et al. Evaluating the use of fatty acid profiles to identify Francisella tularensis[J]. J Microbiol Meth, 2007, 90(2)：465-469.
[18] 吴愉萍, 徐建明, 汪海珍, 等. Sherlock MIS系统应用于土壤细菌鉴定的研究[J]. 土壤学报, 2006, 43(4)：642-648.
[19] 刘波. 微生物脂肪酸生态学[M]. 北京：中国农业科学出版社, 2011：1-798.
[20] 黄朱梁, 裘迪红. MIDI Sherlock微生物自动鉴定系统鉴定方法的建立[J]. 宁波大学学报：理工版, 2011, 24(2)：8-13.