Screening and Identification of Antagonistic Endophytes Against Drug-resistant Bacteria from Medicinal Plants

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.022

Abstract

Abstract: Methicillin-resistant Staphylococcus aureus(MRSA), Cephalosporin-resistant Escherichia coli and Imipenem-resistant Pseudomonas aeruginosa have caused severe infection in clinical therapy and greatly endanger human health. The aim of this study is to isolate and screen highly active antagonistic endophytes against drug-resistant bacteria from medicinal plant. After two-step screening, 18 strains have antagonistic effect on MRSA were obtained from 197 strains isolated from 22 medicinal plants, none of the strains has antagonistic effect on Cephalosporin-resistant Escherichia coli and Imipenem-resistant Pseudomonas aeruginosa；16S rRNA sequence analysis showed that eight strains belong to Streptomyces, six strains belong to Bacillus, and four strains belong to Pseudomonas. Five strains which have strong anti bacterial activity were chosen for fermentation, and the fermented liquid of QN1 have strong inhibition effect on MRSA.

Key words: endophytes, antibacterial activity, drug-resistant bacteria, Methicillin-resistant Staphylococcus aureus

Liu Xiaoyu, Ma Yuchao. Screening and Identification of Antagonistic Endophytes Against Drug-resistant Bacteria from Medicinal Plants[J]. Biotechnology Bulletin, 2015, 31(3): 154-160.

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