Cloning and Expression Analysis of Tobacco Peroxidase Gene NtPOD2

doi:10.13560/j.cnki.biotech.bull.1985.2019-0395

Abstract

Abstract: Peroxidase（POD）is widely present in various organs and different developmental stages of plants,and plays an important role in plant growth,development and coping with stress. In order to explore the function of POD gene family in tobacco,A peroxidase gene was cloned from the common tobacco K326 by gene cloning. The gene was 99% homologous of Nicotiana tomentosiformis POD2,thus it was named NtPOD2 gene. Bioinformatics analysis was used to predict the structure of the NtPOD2 gene,and qRT-PCR technique to determine the expression level of NtPOD2 in different tissues and abiotic stress. Results showed that the sequence contained an open reading frame（ORF）of 897 bp and encoded 298 amino acid residues,and NtPOD2 protein had a type Ⅲ peroxidase typical conserved domain. NtPOD2 gene was expressed in root,stem,leave and flower,but the highest expression in root,and rapidly responded to abiotic stress induction. This indicates that NtPOD2 may be involved in plant growth,development and abiotic stress.

Key words: tobacco, NtPOD2, cloning, expression

WANG Jing, LIU Lun, YANG Yong, HU Sheng, LI Li-qin. Cloning and Expression Analysis of Tobacco Peroxidase Gene NtPOD2[J]. Biotechnology Bulletin, 2019, 35(12): 24-30.

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