cDNA-AFLP Analysis of Dormancy-related Genes in Seed Hypocotyl of Paeonia lactiflora

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.018

Abstract

Abstract: To explore the dormancy mechanism of seed hypocotyl of Paeonia lactiflora and isolate dormancy-related genes from P. lactiflora, a cDNA-AFLP technology was used to identify differentially expressed genes between the stages of seeds with stratification for 0 d and germination. A total of 3 600 differentially expressed transcript-derived fragments(TDFs)were screened by selective PCR amplification using 256 primer combinations, and 1 200 TDFs were recovered successfully. Then selected 500 TDFs were cloned and sequenced, and 42 effective sequences were obtained. Bioinformatics analysis with BLAST showed that 30 TDFs were homologous to the known functional genes, which involved in gene expression regulation, signal transduction, stress responding, material and energy metabolism etc. during the dormancy of seed hypocotyl of P. lactiflora. Nine TDFs were similar to genes of unknown functions and hypothetical proteins, and another 3 TDFs had no sequence homology to GenBank entries and might be new unknown genes, which could help us to clarify the mechanism of seed hypocotyl dormancy of P. lactiflora.

Key words: Paeonia lactiflora, seed, hypocotyl, dormancy, cDNA-AFLP, differentially expressed gene

Sun Xiaomei, Han Ningning, Yang Hongguang, Yang Panpan. cDNA-AFLP Analysis of Dormancy-related Genes in Seed Hypocotyl of Paeonia lactiflora[J]. Biotechnology Bulletin, 2015, 31(6): 116-121.

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