Effects of 17β-estradiol on the Sex Differentiation of Zebrafish(Danio rerio)

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.032

Abstract

Abstract: Previous studies have demonstrated that 17β-estradiol(E2)could increase the female proportion in zebrafish population. In order to study mechanisms of feminization caused by E2, quantitative real-time PCR(qRT-PCR)was used to analyze expression levels of genes involved in sexual differentiation(brca2, sox9a, sox9b, dmrt1 and cyp19a1a)under different conditions. The results indicate that E2 can up-regulate the expression levels of the female-predominant genes(brac2 and sox9b), partially down-regulate the expression levels of male-predominant gene(sox9a)and has different effects on sex hormone conversion pathway(cyp19a1a)in different developmental stages of zebrafish.

Key words: endocrine disrupting chemicals, 17β-estradiol, zebrafish, sexual differentiation

Li Guochao, Yu Kaimin, Feng Weimin, Liu Lili, Zhang Jiayu, Yan Yanchun. Effects of 17β-estradiol on the Sex Differentiation of Zebrafish(Danio rerio)[J]. Biotechnology Bulletin, 2015, 31(6): 200-208.

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