The Application of the PiggyBac Transposon System in mCherry-transgenic Danio rerio

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.027

Abstract

Abstract: The red fluorescent protein gene(mCherry)was inserted into the modified PiggyBac transposon donor plasmid 5'-PTK-3', and the vector plasmid for gene trap, PTK-mCherry was constructed.PTK-mCherry and PiggyBac transposase mRNA were co-injected into fertilized eggs by microinjection, and the zebrafishes expressing mCherry in different temporally and spatially patterns were acquired.The statistics revealed that the expression rate of mCherry in F0 was 8.2%, indicating that PiggyBac transposon efficiently translocated and captured the different genes in zebrafish, and the efficient screening of mutants was achieved.

Key words: PiggyBac transposon, zebrafish, gene trap

CAO Shou-ying, BAI Chang-cun. The Application of the PiggyBac Transposon System in mCherry-transgenic Danio rerio[J]. Biotechnology Bulletin, 2016, 32(2): 185-191.

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