The Regulation of Mecp2 on Notch Signaling Pathway During Early Neural Development of Zebrafish Embryos

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.031

Abstract

Abstract: This study aims to explore the regulation role and mechanism of Mecp2 during early neural development of zebrafish embryos. By the techniques of confocal laser scanning microscopy and in situ hybridization，and using Notch transgenic fish，it was found that Mecp2 widely expressed in the early embryonic development，then gradually expressed in the brain. In the embryos with Mcep2 knockdown，the expression of Notch signal decreased，and both the injection of Mcep2-RNA and intracellular INCD-RNA restored the phenotype. The expression of neural factors of Ngn1，Pax2，Pax3，Eno2，and Map labelling for neural maturation as well as HUC binding with RNA were downregulated. After Mecp2 knocked down，the expression of transcriptional repressor Hey2 downregulated because the knockdown hindered the differentiation and maturation of neural cells. Our study showed that Mecp2 regulated the differentiation of nerve cells of zebrafish through Notch-Hey2 signaling pathways.

Key words: Mecp2, zebrafish, neurodevelopmental, Notch signaling pathway, neural cell differentiation

HE Li-fan ,GAO Hai. The Regulation of Mecp2 on Notch Signaling Pathway During Early Neural Development of Zebrafish Embryos[J]. Biotechnology Bulletin, 2016, 32(4): 228-233.

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