Process Optimization of Extracting Polysaccharides from Onchidium struma,and Evaluation of Its Antioxidant Activity in Vitro

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.027

Abstract

Abstract: Taking Onchidium struma as the raw material, its polysaccharides was extracted by the traditional method of hot water. Process of extracting polysaccharides from O. struma was optimized by response surface methodology. Through the single factor experiment, the effects of extraction temperature, extraction time, and liquor-to-material ratio to the extraction rate of polysaccharide were investigated. Furthermore, the experiments were done by Box-Behnken, and the data was analyzed by Design-Expert 8.05 software. Antioxidant activity of O. struma polysaccharides was determined by the capacity of scavenging free hydroxyl radical and DPPH radical. On the basis of the analysis of regression equation, determination of factors affecting the extraction of polysaccharide was conducted by the extraction rate of polysaccharide served as response value. Extraction temperature at 92℃, extraction time of 30 h, and liquor-to-material ratio of 1∶29 g/mL, leaching rate of O. struma polysaccharide reached 9.206%. O. struma polysaccharide had ability to clear off free hydroxyl radical and DPPH radical. Conclusively, the extraction technology is reasonable and reliable for the extraction of polysaccharides O. struma, which provides the theoretical support for the future industrial production, and its polysaccharides have significant antioxidation in vitro.

Key words: Onchidium struma, polysaccharide, extraction, response surface method, antioxidation effect

Cheng Zhiqing, Shen Heding, Yao Lixiang, Diao Ya, Liu Chen. Process Optimization of Extracting Polysaccharides from Onchidium struma,and Evaluation of Its Antioxidant Activity in Vitro[J]. Biotechnology Bulletin, 2015, 31(8): 186-192.

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