Application Prospect of Genetic Engmeering and Metabolic Engineering in Improving Its Production of Steviol Glycoside

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.002

Abstract

Abstract: Stevia sugar is a unique glycoside produced from stevia(Stevia rebaudiana)of a perennial herb originally growing in South America. Sweet tea tree(Rubus suavissimus), mainly growing in the Guangxi area of China, also produces similar glycoside. In recent years, stevia sugar as a natural high-intensity sweetener has increasingly been used in USA, European countries, Japan, and China. Stevia is extensively cultivated in China. Here we review the current status of stevia production and usage of stevia sugar, as well as progresses on understanding its biosynthetic pathway. Recent advances on identifying the enzymes involved in steviol biosynthesis, isolating relevant genes as well establishing stevia transformation system provide the fundamentals toward improving the production of steviol glycoside and altering the constitute of stevia glycoside through genetic and metabolic engineering, also inspire the novel measures of producing stevia sugar using microorganisms and high biomass crops.

Key words: Stevia rebaudiana, stevia sugar, sweetener, metabolic engineering, genetic engineering

Yu Boying, Wang Ninglin, Li Guojing, Xia Yiji. Application Prospect of Genetic Engmeering and Metabolic Engineering in Improving Its Production of Steviol Glycoside[J]. Biotechnology Bulletin, 2015, 31(9): 8-14.

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