Clone and Expression Analysis of The High-affinity K+ Transporter Gene SeHKT1 from the Halophyte Salicornia europaea

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.021

Abstract

Abstract: A putative high-affinity potassium transporter gene named SeHKT1 was cloned from halophyte Salicornia europaea using RACE techniques with GenBank number of KP739261. The characteristics of sequence was analyzed by bioinformatics and the expression patterns were verified by Real-time PCR in different tissues and under different salt stresses. The results showed that the SeHKT1 was highly closely related to SbHKT1 of Sorghum bicolor and with a 1 761 bp open reading frame(ORF)and encoding a polypeptide of 550 amino acids. Real-time PCR results showed that the SeHKT1 was expressed both in shoot and root by NaCl stimulus, but the transcription level were higher in root than shoot. The expression level reached highest level at 6 h and decreased after this time point in root under 200 mmol/L NaCl solution treatment. Under potassium starvation condition, the SeHKT1 was also induced in both of roots and shoots with high level. All of these results demonstrate SeHKT1 not only response NaCl stresses, but also regulate potassium absorption under potassium starvation environment.

Key words: Salicornia europaea, high-affinity potassium transporter, RACE, gene expression, salt-tolerance

Ma Jinbiao, Zhang Dayong, Zhang Meiru, Xiao Xinlong , Zhang Xuan, Li Li. Clone and Expression Analysis of The High-affinity K⁺ Transporter Gene SeHKT1 from the Halophyte Salicornia europaea[J]. Biotechnology Bulletin, 2015, 31(11): 159-165.

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Clone and Expression Analysis of The High-affinity K⁺ Transporter Gene SeHKT1 from the Halophyte Salicornia europaea

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