Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae

doi:10.13560/j.cnki.biotech.bull.1985.2016-1209

Abstract

Abstract: Rice blast disease,caused by ascomycete Magnaporthe oryzae,is one of the most devastating diseases threatening rice production. Understanding its pathogenic mechanism not only provides strategies for disease control,but also generates new knowledge of fungal pathogenesis. Mechanosensitive channels,the window of material exchange,can be the biocontrol targets for disease control. These channels can sense changes of membrane tension and transduce this signal into intracellular environment. In this study,we identified three mechanosensitive channels（MoMsc1,MoMid1,and MoYvc1）in M. oryzae by bioinformatics method. The results showed that the three genes were evolutionarily conserved in fungi. Furthermore,we discovered that silence of MoMsc1,encoding the putative gene of mechanosensitive channel of small conductance in M. oryzae,did not affect its vegetative growth,asexual development,stress response,cell wall integrity and virulence.

Key words: Magnaporthe oryzae, mechanosensitive channel, mechanosensitive channel of small conductance

TAO Shang-kun, WANG Guo-liang, LIU Wen-de. Identification and Functional Analysis of Mechanosensitive Channels in Magnaporthe oryzae[J]. Biotechnology Bulletin, 2017, 33(6): 97-103.

References

[1] Ebbole DJ. Magnaporthe as a model for understanding host-pathogen interactions[J]. Annual Review of Phytopathology, 2007, 45（1）：437-456.
[2] Wilson RA, Talbot NJ. Under pressure：investigating the biology of plant infection by Magnaporthe oryzae[J]. Nature Reviews Microbiology, 2009, 7（3）：185-195.
[3] Sackin H. Mechanosensitive channels[J]. Annual Review of Physiology, 1995, 57（1）：333-353.
[4] Martinac B. Mechanosensitive ion channels：molecules of mechanotransduction[J]. J Cell Sci, 2004, 117（12）：2449-2460.
[5] Bass RB, Strop P, Barclay M, et al. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel[J]. Science, 2002, 298（5598）：1582-1587.
[6] Booth IR, Paul B. The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves[J]. Journal of Bacteriology, 2012, 194（18）：4802-4809.
[7] Haswell ES. MscS-like proteins in plants[J]. Current Topics in Membranes, 2007, 58（6）：329-359.
[8] Lee CP, Maksaev G, Jensen GS, et al. MSL1 is a mechanosensitive ion channel that dissipates mitochondrial membrane potential and maintains redox homeostasis in mitochondria during abiotic stress[J]. Plant Journal, 2016, 88（5）：809-825.
[9] Haswell ES, Meyerowitz EM. MscS-like proteins control plastid size and ahape in Arabidopsis thaliana[J]. Current Biology, 2006, 16（1）：1-11.
[10] Hamilton ES, Jensen GS, Maksaev G, et al. Mechanosensitive channel MSL8 regulates osmotic forces during pollen hydration and germination[J]. Science, 2015, 350（6259）：438-441.
[11] Haswell ES, Peyronnet R, Barbier-Brygoo H, et al. Two MscS homologs provide mechanosensitive channel activities in the Arabidopsis root[J]. Current Biology, 2008, 18（10）：730-734.
[12] Veley KM, Grigory M, Frick EM, et al. Arabidopsis MSL10 has a regulated cell death signaling activity that is separable from its mechanosensitive ion channel activity[J]. Plant Cell, 2014, 26 （7）：3115-3131.
[13] Yoshitaka N, Kenta F, Masahiro S, et al. Molecular and electrophysiological characterization of a mechanosensitive channel expressed in the chloroplasts of Chlamydomonas[J]. Proc Natl Acad Sci USA, 2007, 104（14）：5883-5888.
[14] Nakayama Y, Yoshimura K, Iida H. Organellar mechanosensitive channels in fission yeast regulate the hypoosmotic shock response[J]. Nature Communications, 2012, 3（8）：2543-2544.
[15] Peiter E, Fischer MK, Roberts SK, et al. The Saccharomyces cerevisiae Ca 2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity[J]. Febs Letters, 2005, 579（25）：5697-5703.
[16] Paidhungat M, Garrett S. A homolog of mammalian, voltage-gated calcium channels mediates yeast pheromone-stimulated Ca 2+ uptake and exacerbates the cdc1（Ts）growth defect[J]. Molecular & Cellular Biology, 1997, 17（11）：6339-6347.
[17] Lew RR, Abbas Z, Anderca MI, et al. Phenotype of a mechanosensitive channel mutant, mid-1, in a filamentous fungus, Neurospora crassa[J]. Eukaryotic Cell, 2008, 7（4）：647-655.
[18] Cavinder B, Hamam A, Lew RR, et al. Mid1, a mechanosensitive calcium ion channel, affects growth, development, and ascospore discharge in the filamentous fungus Gibberella zeae[J]. Eukaryotic Cell, 2011, 10（6）：832-841.
[19] Bormann J, Tudzynski P. Deletion of Mid1, a putative stretch-activated calcium channel in Claviceps purpurea, affects vegetative growth, cell wall synthesis and virulence[J]. Microbiology, 2009, 155（Pt 12）：3922-3933.
[20] de Castro PA, Chiaratto J, Winkelströter LK, et al. The involvement of the Mid1/Cch1/Yvc1 calcium channels in Aspergillus fumigatus virulence[J]. PLoS One, 2014, 9（9）：e103957.
[21] Palmer CP, Zhou XL, Lin J, et al. A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca（2+）-permeable channel in the yeast vacuolar membrane[J]. Proceedings of the National Academy of Sciences, 2001, 98（14）：7801-7805.
[22] Yu Q, Fan W, Qiang Z, et al. A novel role of the vacuolar calcium channel Yvc1 in stress response, morphogenesis and pathogenicity of Candida albicans[J]. International Journal of Medical Microbiology, 2013, 304（3）：339-350.
[23] Lange M, Weihmann F, Schliebner I, et al. The transient receptor potential（TRP）channel family in Colletotrichum graminicola：a molecular and physiological analysis[J]. PLoS One, 2016, 11 （6）：e0158561.
[24] Watts HJ, Véry AA, Perera TH, et al. Thigmotropism and stretch-activated channels in the pathogenic fungus Candida albicans[J]. Microbiology, 1998, 144（Pt 3）（3）：689-695.
[25] Yuko N, Takeshi K, Kazuo S, et al. Arabidopsis plasma membrane protein crucial for Ca 2+ influx and touch sensing in roots[J]. Proc Natl Acad Sci USA, 2007, 104（104）：3639-3644.
[26] Valent B, Chumley FG. Molecular genetic analysis of the rice blast fungus, Magnaporthe grisea[J]. Annual Review of Phytopathology, 1991, 29（29）：443-467.
[27] Nakayashiki H, Hanada S, Nguyen BQ, et al. RNA silencing as a tool for exploring gene function in ascomycete fungi[J]. Fungal Genetics & Biology, 2005, 42（4）：275-283.
[28] Liedtke W, Friedman JM. Abnormal osmotic regulation in trpv4-/- mice[J]. Proceedings of the National Academy of Sciences, 2003, 100（23）：13698-13703.
[29] Sung EK, Bertrand C, Abhishek C, et al. The role of Drosophila Piezo in mechanical nociception[J]. Nature, 2012, 483（7388）：209-212.
[30] Booth IR, Miller S, Müller A, et al. The evolution of bacterial mechanosensitive channels[J]. Cell Calcium, 2014, 57（3）：140-150.
[31] Jacob S, Foster AJ, Yemelin A, et al. High osmolarity glycerol（HOG）signalling in Magnaporthe oryzae：Identification of MoYPD1, and its role in osmoregulation, fungicide action, and pathogenicity[J]. Fungal Biology, 2015, 119（7）：580-594.
[32] Dezwaan TM, Carroll AM, Valent B, et al. Magnaporthe grisea pth11p is a novel plasma membrane protein that mediates appressorium differentiation in response to inductive substrate cues[J]. Plant Cell, 1999, 11（10）：2013-2030.
[33] Liu H, Suresh A, Willard FS, et al. Rgs1 regulates multiple Galpha subunits in Magnaporthe pathogenesis, asexual growth and thigmotropism[J]. Embo Journal, 2007, 26（3）：690-700.