Isolation and Identification of Bacillus amyloliquefaciens HR-2 and Biological Control of Rice Blast

doi:10.13560/j.cnki.biotech.bull.1985.2020-0970

Abstract

Abstract:

Magnaporthe oryzae is a rice fungal disease that seriously threatens global food security. A strain HR-2 with high antagonistic activity against M. oryzae was screened from Yueyang district,Hunan province by plate confrontation method in this study. The strain HR-2 was identified as Bacillus amyloliquefaciens by morphological verification,16S rRNA,gyrA and tuf gene sequence analysis. The results of broad-spectrum inhibition showed that the strain HR-2 had a fine inhibitory effect on M. oryzae,Sclerotinia sclerotiorum and Ralstonia solanacearum,and the inhibition rates were 82.34%,72.32% and 72.64%,respectively. The results of pot experiment demonstrated that the control effects of HR-2 10-times diluted solution and 100-times diluted solution on M. oryzae were 63.81% and 40.77% respectively,among which the control effects of HR-2 10-times diluted solution were similar to that of 50 mg/L azoxystrobin. The above results indicated that B. amyloliquefaciens HR-2 had a wide antibacterial spectrum and an obvious control effect on M. oryzae. Our research lays a foundation for the development of biological control agent against M. oryzae .

Key words: Magnaporthe oryzae, Bacillus amyloliquefaciens, isolated and identification, biological control

LI Jin, PENG Ke-wei, PAN Qiu-yi, ZHU Zhe-yuan, PENG Di. Isolation and Identification of Bacillus amyloliquefaciens HR-2 and Biological Control of Rice Blast[J]. Biotechnology Bulletin, 2021, 37(3): 27-34.

Figures/Tables 11

References 26

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引物名称	引物序列（5'-3'）
27 F	AGAGTTTGATCCTGGCTCAG
1492 R	GGTTACCTTGTTACGACTT
gyrA F	CAGTCAGGAAATGCGTACGTCCTT
gyrA R	CAAGGTAATGCTCCAGGCATTGCT
tuf GPF	ACGTTGACTGCCCAGGACAC
tuf GPR	GATACCAGTTACGTCAGTTGTACGGA

引物名称	引物序列（5'-3'）
27 F	AGAGTTTGATCCTGGCTCAG
1492 R	GGTTACCTTGTTACGACTT
gyrA F	CAGTCAGGAAATGCGTACGTCCTT
gyrA R	CAAGGTAATGCTCCAGGCATTGCT
tuf GPF	ACGTTGACTGCCCAGGACAC
tuf GPR	GATACCAGTTACGTCAGTTGTACGGA

供试病原菌	HR-2	G-1	G-2	G-3	G-4	G-10	HR-8
水稻稻瘟病菌	82.34±0.08a	82.54±0.04a	82.74±0.06a	83.93±0.06a	82.14±0.18a	81.55±0.16a	82.44±0.04a
油菜菌核病菌	72.32±0.04b	68.25±0.68a	68.06±0.68a	68.65±0.69a	/	/	/
番茄青枯病菌	72.62±0.02a	76.19±0.02a	73.81±0.01a	73.81±0.01a	/	/	/
小麦赤霉病菌	21.05±0.2a	/	13.16±0.05a	31.58±0.1a	/	/	/
西瓜枯萎病菌	26.32±0.16a	/	/	/	/	/	/

供试病原菌	HR-2	G-1	G-2	G-3	G-4	G-10	HR-8
水稻稻瘟病菌	82.34±0.08a	82.54±0.04a	82.74±0.06a	83.93±0.06a	82.14±0.18a	81.55±0.16a	82.44±0.04a
油菜菌核病菌	72.32±0.04b	68.25±0.68a	68.06±0.68a	68.65±0.69a	/	/	/
番茄青枯病菌	72.62±0.02a	76.19±0.02a	73.81±0.01a	73.81±0.01a	/	/	/
小麦赤霉病菌	21.05±0.2a	/	13.16±0.05a	31.58±0.1a	/	/	/
西瓜枯萎病菌	26.32±0.16a	/	/	/	/	/	/

处理	病情指数	防效/%
CK	46.75±0.35a	∕
100 times dilution	27.74±1.07b	40.77±1.91b
10 times dilution	16.96±0.92c	63.81±1.93a
50 mg/L Azoxystrobin	16.18±0.87c	65.48±1.84a